【摘要】：為緩解國際魚油資源短缺,本研究從血脂代謝角度去評價(jià)豆油替代魚油在中華條頸龜中的適宜比例。選取體質(zhì)量(1557±307)g的4齡雌性成體中華條頸龜,根據(jù)日糧中豆油與魚油的添加比例不同隨機(jī)分成四組,0S∶3F組(豆油∶魚油0∶3),1S∶2F組(豆油∶魚油1∶2),2S∶1F組(豆油∶魚油2∶1)和3S∶0F組(豆油∶魚油3∶0);外源油的添加量占日糧1%。每周投喂2次,連續(xù)投喂8個(gè)月。從每組中隨機(jī)取6只中華條頸龜,冷凍麻醉處死,解剖。檢測血液中甘油三酯、總膽固醇、高密度脂蛋白膽固醇和低密度脂蛋白膽固醇含量,肝臟組織中脂蛋白脂酶、肝脂酶和激素敏感性脂肪酶活性以及腸道胰脂肪酶活性,結(jié)果如下:(1)隨著豆油替代比例的升高,只有1S∶2F組和3S∶0F組總膽固醇顯著高于0S∶3F組,并且3S∶0F組低密度脂蛋白含量顯著高于0S∶3F(P0.05)。(2)總脂酶活力在2S∶1F組達(dá)到最高,顯著高于0S∶3F組(P0.05);2S∶1F組的激素敏感性脂肪酶活力與其余組相比,更接近于0S∶3F;胰脂肪酶的活力在2S∶1F與0S∶3F之間差異不顯著,但均顯著低于其余兩組。綜合上述兩方面指標(biāo)來看,豆油替代魚油在中華條頸龜中總體可行,較適宜的比例組為2S∶1F。
[Abstract]:In order to alleviate the shortage of international fish oil resources, this study evaluated the suitable proportion of soybean oil instead of fish oil in Chinese tortoise from the point of lipid metabolism. A 4-year-old female adult female necked turtle (1557 鹵307g) was randomly divided into four groups: 0S:3F group (soybean oil: fish oil 0:3) and 1S:2F group (soybean oil: fish oil 1:2). 2S:1F group (soybean oil: fish oil 2:1) and 3S:0F group (soybean oil: fish oil 3:0); The addition of exogenous oil accounted for 1% of the diet. Feed twice a week for 8 months. Six Chinese tortoises were randomly selected from each group and killed and dissected by freezing anesthesia. The contents of triglyceride, total cholesterol, high density lipoprotein cholesterol and low density lipoprotein cholesterol in blood, lipoprotein lipase, liver lipase and hormone sensitive lipase activity in liver tissue and pancreatic lipase activity in intestine were measured. The results were as follows: (1) with the increase of the substitution ratio of soybean oil, only the total cholesterol in 1S:2F and 3S:0F groups was significantly higher than that in 0S:3F group. The content of low density lipoprotein in 3S:0F group was significantly higher than that in 0S:3F group (P0.05). (2), and the activity of total lipase in 2S:1F group was the highest, significantly higher than that in 0S:3F group (P0.05). The activity of glucocorticoid sensitive lipase in 2S:1F group was closer to 0: 3F than that in other groups, and the activity of pancreatic lipase in 2S:1F and 0S:3F was not significantly different from that in other two groups, but was significantly lower than that in the other two groups. According to the above two indexes, the substitution of soybean oil for fish oil is generally feasible in Chinese tortoise, and the more suitable proportion is 2S: 1F.
【作者單位】： 海南師范大學(xué)生命科學(xué)學(xué)院;中國科學(xué)院成都生物研究所;
【基金】：國家自然科學(xué)基金資助項(xiàng)目(31360642,31372228)
【分類號(hào)】：S966.5

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本文編號(hào)：2390308