【摘要】：目的：建立鏈脲佐菌素（Streptozotocin）誘導(dǎo)的2型糖尿�。―iabetes Melitus）大鼠模型，觀察姜黃素(Curcumin)對(duì)2型糖尿病大鼠肺組織損傷的保護(hù)作用和P38MAPK蛋白表達(dá)的影響，探討其可能的作用機(jī)制，為其臨床價(jià)值提供理論依據(jù)。 方法:雄性wistar大鼠隨機(jī)分為三組：正常對(duì)照組（Control）、糖尿病組（DM)、糖尿病+姜黃素治療組(DM+CUR)。糖尿病組與藥物干預(yù)組大鼠腹腔注射STZ建立糖尿病模型。成膜后開(kāi)始給予姜黃素，于15周后測(cè)定大鼠血糖和體重，大鼠麻醉處理后，取右肺中葉低溫冷凍速凍，同時(shí)取右肺下葉置于甲醛溶液中固定。采用蘇木素-伊紅（HE）染色觀察大鼠糖尿病肺組織形態(tài)學(xué)改變情況。免疫組織化學(xué)法（s-p法）和免疫印跡法檢測(cè)肺組織中p38MAPK和TGF-β1蛋白的表達(dá)水平。 結(jié)果：1、糖尿病組大鼠與對(duì)照組相比血糖顯著升高，體重下降。姜黃素組比糖尿病大鼠血糖降低、體重下降緩慢。 2、光鏡下正常組肺組織結(jié)構(gòu)清晰，管壁以及粘膜上皮完整。糖尿病組大鼠肺組織結(jié)構(gòu)紊亂，肺泡上皮細(xì)胞變性、肺泡腔縮小，肺泡壁結(jié)構(gòu)不完整，間隔毛細(xì)血管基底膜增厚、管腔狹窄；纖維結(jié)締組織增生，少許炎細(xì)胞浸潤(rùn)。姜黃素治療組大鼠肺組織損傷較糖尿病組明顯減輕。 3、免疫組化(s-p染色)結(jié)果顯示糖尿病組棕黃色陽(yáng)性顆粒比對(duì)照組多，姜黃素組次之。免疫熒光染色顯示p-p38MAPK表達(dá)與s-p染色結(jié)果相似。免疫蛋白印跡結(jié)果糖尿病組與對(duì)照組相比肺組織中的p38MAPK及蛋TGF-β1白表達(dá)增強(qiáng)（P0.05）。姜黃素組大鼠肺組織中的p38MAPK及TGF-β1蛋白表達(dá)較糖尿病組顯著下降（P0.05）。 結(jié)論：姜黃素可降低糖尿病大鼠血糖，改善糖尿病大鼠體重，減輕糖尿病大鼠肺組織的病理?yè)p傷，，其機(jī)制可能部分通過(guò)影響p38MAPK和TGF-β1蛋白表達(dá)有關(guān)。
[Abstract]:Aim: to establish a rat model of type 2 diabetic (Diabetes Melitus) induced by streptozotocin (Streptozotocin), to observe the protective effect of curcumin (Curcumin) on lung tissue injury and the expression of P38MAPK protein in type 2 diabetic rats, and to explore its possible mechanism. To provide the theoretical basis for its clinical value. Methods: male wistar rats were randomly divided into three groups: normal control group, (Control), diabetes group, (DM), diabetic curcumin treatment group, (DM CUR). Diabetic model was established by intraperitoneal injection of STZ in diabetic group and drug intervention group. After membrane formation curcumin was given to rats. After 15 weeks blood glucose and body weight were measured. After anesthesia the middle lobe of the right lung was frozen and the lower lobe of the right lung was fixed in formaldehyde solution. The changes of lung histomorphology in diabetic rats were observed by hematoxylin-eosin (HE) staining. The expression of p38MAPK and TGF- 尾 1 protein was detected by immunohistochemistry and Western blotting. Results: 1. Compared with the control group, the diabetic rats significantly increased their blood glucose and lost their body weight. Curcumin group had lower blood glucose and slower weight loss than diabetic rats. 2. The lung tissue structure was clear and the wall and mucosal epithelium were intact in the normal group under light microscope. In the diabetic group, the pulmonary tissue structure was disorder, alveolar epithelial cell degeneration, alveolar cavity shrinking, alveolar wall structure incomplete, capillary basement membrane thickening, lumen stenosis, fibrous connective tissue hyperplasia, a little inflammatory cell infiltration. The lung tissue injury in curcumin treatment group was significantly less than that in diabetic group. 3. The results of immunohistochemistry (s-p staining) showed that there were more brown positive granules in diabetic group than in control group, followed by curcumin group. Immunofluorescence staining showed that the expression of p-p38MAPK was similar to that of s-p staining. Compared with the control group, the expression of p38MAPK and egg TGF- 尾 1 in the diabetic group was higher than that in the control group (P0.05). The expression of p38MAPK and TGF- 尾 1 protein in curcumin group was significantly lower than that in diabetic group (P0.05). Conclusion: curcumin can decrease the blood sugar, improve the body weight and reduce the pathological damage of lung tissue in diabetic rats. The mechanism may be related to the expression of p38MAPK and TGF- 尾 1 protein.
【學(xué)位授予單位】：湖北科技學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：R285.5

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 付秀美;王曉杰;鐘美蓉;馬洪偉;陳志宏;薛景鳳;;中藥治療糖尿病機(jī)制研究進(jìn)展[J];承德醫(yī)學(xué)院學(xué)報(bào);2009年04期

2 田華;胡靈衛(wèi);張玉霞;蘆琨;;姜黃素對(duì)糖尿病腎病大鼠腎臟的抗氧化應(yīng)激作用及保護(hù)機(jī)制[J];中國(guó)臨床藥理學(xué)雜志;2013年06期

3 夏城東,丁學(xué)屏,葉偉成;糖尿病腎病與多元醇通路[J];中國(guó)中西醫(yī)結(jié)合腎病雜志;2001年11期

4 修麗梅;王振平;嚴(yán)雪梅;張麗娜;魏軍平;施萍;王亞娜;;糖尿病足微血管病變及其藥物治療的研究進(jìn)展[J];世界中西醫(yī)結(jié)合雜志;2012年06期

5 殷松樓;周冬梅;奚玨;殷寒秋;;2型糖尿病患者氧化應(yīng)激指標(biāo)的變化[J];徐州醫(yī)學(xué)院學(xué)報(bào);2010年01期

6 李玉賢;張艷麗;左靜靜;尚遂存;;黃連素治療糖尿病的臨床應(yīng)用[J];中國(guó)醫(yī)藥指南;2012年08期

7 狄建彬;顧振綸;趙笑東;錢培剛;蔣小崗;郭次儀;;姜黃素的抗氧化和抗炎作用研究進(jìn)展[J];中草藥;2010年05期

8 劉紅艷;王海燕;葉松;郭靜明;;姜黃素藥理作用及其機(jī)制研究進(jìn)展[J];中國(guó)現(xiàn)代醫(yī)學(xué)雜志;2012年06期

9 鄧曉威;謝寧;;黃連素治療2型糖尿病研究進(jìn)展[J];中國(guó)中藥雜志;2014年08期

10 李莎;白育庭;;糖尿病肺損傷的研究進(jìn)展[J];湖北科技學(xué)院學(xué)報(bào)(醫(yī)學(xué)版);2014年03期

本文編號(hào)：2332922