【摘要】：背景:有研究表明肝硬化個體在行肝部分切除后肝再生較健康肝臟緩慢的原因可能與其肝再生過程中的肝細(xì)胞生長因子的合成分泌延遲及活性降低有關(guān),但導(dǎo)致這一現(xiàn)象的原因尚不明確,近年來發(fā)現(xiàn)的肝細(xì)胞生長因子激活因子的抑制因子(hepatocyte growth factor activator inhibitor,HAI)能夠間接地抑制肝細(xì)胞生長因子的激活,但是鮮有研究探索HAI的抑制因子在肝硬化肝臟行部分肝切除后肝再生過程中的表達(dá)情況及其與肝再生的關(guān)系。目的:通過建立肝硬化大鼠部分肝切除模型來研究HAI的抑制因子1,2(HAI-1,HAI-2)在硬化和正常肝臟再生中的表達(dá)情況,探討HAI-1,HAI-2在硬化肝臟部分肝切除后的生物學(xué)效應(yīng)及其與肝再生的關(guān)系。方法:采用體積分?jǐn)?shù)40%四氯化碳油溶液背部皮下注射法建立大鼠肝硬化模型,對實驗組進行70%肝臟切除術(shù)建立再生模型,對照組大鼠僅給予普通飼料飲水喂養(yǎng)并行70%肝臟切除。分別于術(shù)前及術(shù)后3,6,12,24,48 h麻醉處死大鼠并留取脾臟標(biāo)本檢測。采用RT-PCR技術(shù)檢測脾源性HAI-1,HAI-2 mR NA的表達(dá)情況。結(jié)果與結(jié)論:兩組大鼠部分肝切除后HAI-1 mR NA的表達(dá)均呈現(xiàn)出先升高后降低的趨勢,而肝硬化大鼠HAI-1mR NA表達(dá)量持續(xù)高于對照組(P0.05),兩組大鼠之間HAI-2 mRNA表達(dá)量差異無顯著性意義(P0.05)。說明肝硬化大鼠部分肝切除后再生過程中HAI-1 mR NA的表達(dá)持續(xù)高于健康大鼠,這可能導(dǎo)致了肝硬化大鼠肝細(xì)胞生長因子激活因子合成分泌不足,從而使得肝細(xì)胞生長因子前體活化不足,最終引起肝再生緩慢。HAI-2并沒有參與肝臟的損傷修復(fù)進程。
[Abstract]:Background: studies have shown that the slow regeneration of liver after partial hepatectomy may be related to the delayed synthesis and secretion of hepatocyte growth factor (HGF) and decreased activity of hepatocyte growth factor (HGF) during liver regeneration in cirrhotic individuals. However, the cause of this phenomenon is not clear. Recently, the inhibitor of hepatocyte growth factor activator (hepatocyte growth factor activator inhibitor,HAI) can indirectly inhibit the activation of hepatocyte growth factor. However, few studies have explored the expression of HAI inhibitor in liver regeneration after partial hepatectomy in cirrhotic liver and its relationship with liver regeneration. Objective: to study the expression of HAI inhibitor 1t2 (HAI-1,HAI-2) in sclerosing and normal liver regeneration in cirrhotic rats by establishing partial hepatectomy model, and to investigate the biological effect of HAI-1,HAI-2 after partial hepatectomy and its relationship with liver regeneration. Methods: the cirrhotic model of rats was established by subcutaneous injection of 40% carbon tetrachloride oil solution. The regenerative model was established by 70% hepatectomy in the experimental group and 70% hepatectomy was performed in the control group. The rats were anesthetized for 48 hours before and after operation. The expression of splenic HAI-1,HAI-2 mR NA was detected by RT-PCR technique. Results and conclusion: after partial hepatectomy, the expression of HAI-1 mR NA in the two groups increased first and then decreased, while the expression of HAI-1mR NA in cirrhotic rats was higher than that in the control group (P0.05). There was no significant difference in the expression of HAI-2 mRNA between the two groups (P0.05). The results suggest that the expression of HAI-1 mR NA in liver cirrhosis rats is higher than that in healthy rats after partial hepatectomy, which may lead to insufficient synthesis and secretion of hepatocyte growth factor activator in cirrhotic rats. As a result, the precursor of hepatocyte growth factor was not activated enough, resulting in slow liver regeneration. HAI-2 did not participate in the process of liver injury and repair.
【作者單位】： 天津市第一中心醫(yī)院肝膽外科;天津醫(yī)科大學(xué)一中心臨床學(xué)院;
【基金】：天津市應(yīng)用基礎(chǔ)與前沿技術(shù)研究計劃(14JCYBJC24800) 國家自然科學(xué)基金(81370576)~~
【分類號】：R657.3

【共引文獻(xiàn)】

相關(guān)期刊論文 前10條

1 朱正志;王圣應(yīng);吳禮高;承澤農(nóng);;肝細(xì)胞生長因子在甲狀腺乳頭狀癌的表達(dá)及其與淋巴管生成的關(guān)系[J];蚌埠醫(yī)學(xué)院學(xué)報;2009年05期

2 黃峰;花蓮英;張志堅;劉文國;;肝硬化肝切除術(shù)后肝再生及評估指標(biāo)研究[J];佛山科學(xué)技術(shù)學(xué)院學(xué)報(自然科學(xué)版);2011年01期

3 王麗;劉澤兵;鄭時玉;羅金芳;董軍明;;肝細(xì)胞生長因子受體c-met在甲狀腺乳頭狀癌中的表達(dá)[J];中國耳鼻咽喉頭頸外科;2012年09期

4 李珊珊;李璐;張丹丹;馮霞;丁美;陳煜;;內(nèi)毒素誘導(dǎo)正常人單核細(xì)胞活化中CD69、TNF-α、IL-6的表達(dá)變化及意義[J];北京醫(yī)學(xué);2013年12期

5 詹琪;聶玉強;周永健;;核受體Nur77缺失促進肝再生早期肝細(xì)胞增殖[J];廣州醫(yī)藥;2014年04期

6 邵峰;黃強;王成;邱陸軍;胡元國;查淑云;;雙金屬支架對于晚期惡性肝門部梗阻的療效分析[J];肝膽外科雜志;2014年06期

7 Bojan Stanimirov;Karmen Stankov;Momir Mikov;;Bile acid signaling through farnesoid X and TGR5 receptors in hepatobiliary and intestinal diseases[J];Hepatobiliary & Pancreatic Diseases International;2015年01期

8 Hang-Yu Luo;Shan-Fang Ma;Ji-Fu Qu;De-Hu Tian;;Effects of Kupffer cell inactivation on graft survival and liver regeneration after partial liver transplantation in rats[J];Hepatobiliary & Pancreatic Diseases International;2015年01期

9 薛麗芳;孫蓬明;項雙衛(wèi);;Matriptase與子宮內(nèi)膜癌侵襲轉(zhuǎn)移相關(guān)性的研究進展[J];國際婦產(chǎn)科學(xué)雜志;2015年02期

10 閔愛萍;謝桂清;冷開容;袁麗萍;楊蓉;李英勇;;Hepsin、Maspin在宮頸疾病中的表達(dá)及其臨床意義[J];中國計劃生育和婦產(chǎn)科;2013年06期

相關(guān)博士學(xué)位論文 前10條

1 郭金虎;人類新基因識別與蛋白質(zhì)結(jié)構(gòu)域鑒定[D];復(fù)旦大學(xué);2004年

2 蘇燕;低氧誘導(dǎo)因子-1對肝細(xì)胞生長因子基因表達(dá)調(diào)控機制的研究[D];中國協(xié)和醫(yī)科大學(xué);2006年

3 孫東，

本文編號：2262891