【摘要】：目的建立經(jīng)胃內(nèi)鏡及腹腔鏡腹腔探查的雄兔模型,對比兩者對急性炎性反應的影響。方法雄性日本大耳白兔隨機分為經(jīng)胃內(nèi)鏡(N組,n=8)及腹腔鏡(L組,n=8)腹腔探查組,假手術組(S組)5只,酶聯(lián)免疫吸附試驗(ELISA)檢測術前、術后4、24及72 h血清腫瘤壞死因子-α(TNF-α)、白細胞介素(IL)-6及IL-10的水平。結果動物模型建立成功,N組手術時間較L組長,前者出現(xiàn)胃造口漏1例;TNF-α、IL-6及IL-10水平在術前各組間及S組麻醉前后差異無統(tǒng)計學意義;N、L組TNF-α術后4 h明顯上升,N組上升滯后,但維持時間長,術后4 h L組高于N組,術后24 h相反;N、L組IL-6術后24 h明顯上升,N組術后72 h下降,L組不明顯,術后4 h L組低于N組,術后72 h相反;N組術后IL-10較術前變化不明顯,L組術后24 h明顯下降,持續(xù)至72h,術后24及72 h N組均高于L組。結論經(jīng)胃內(nèi)鏡及腹腔鏡腹腔探查的雄兔模型是可行的,前者耗時較長,胃造口漏是嚴重的并發(fā)癥;兩者均引發(fā)全身促炎反應,但出現(xiàn)及維持時間存在差異,前者抗炎反應變化不明顯,后者術后24及72 h持續(xù)低下。
[Abstract]:Objective to establish a male model of intraperitoneal exploration by endoscopy and laparoscopy, and to compare the effects of the two models on acute inflammatory reaction. Methods male Japanese large ear white rabbits were randomly divided into two groups: intraperitoneal exploration group (group N) and laparoscopy (group L), and sham operation group (group S). (ELISA) was detected by Elisa before operation. Serum levels of tumor necrosis factor- 偽 (TNF- 偽), interleukin (IL) 6 and IL-10 at 24 and 72 hours after operation. Results the operation time of group N was longer than that of group L, the level of TNF- 偽 IL-6 and IL-10 in 1 case with gastric leakage was not significantly different between each group before operation and before and after anesthesia in group S. The increase of TNF- 偽 in group N was significantly higher than that in group L 4 h after operation. However, the maintenance time was longer, 4 h after operation in L group was higher than that in N group, on the contrary, 24 h after operation, the IL-6 in group N increased significantly, and the decrease of IL-6 in group N at 72 h after operation was not obvious, but that in group L was lower than that in group N at 4 h after operation. On the contrary, the IL-10 of group N was significantly lower than that of group L at 24 h after operation, and lasted for 72 h, and that of group N was higher than that of group L at 24 h and 72 h postoperatively. Conclusion it is feasible to explore the male rabbit model by gastroendoscopy and laparoscopy, the former takes a long time and the gastric leakage is a serious complication, both of them cause systemic inflammatory reaction, but there are differences in the time of appearance and maintenance. The former had no obvious change in anti-inflammatory reaction, while the latter had a low level at 24 and 72 hours after operation.
【作者單位】： 南昌大學第二附屬醫(yī)院肝膽外科;
【基金】：江西省衛(wèi)生廳科技計劃項目(編號:20094005)
【分類號】：R57

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