【摘要】：皮膚中角質層與其他層面阻抗的巨大差異,使得將皮膚作為一個整體的阻抗特性研究較難開展。研究在體條件下無創(chuàng)或微創(chuàng)地檢測與分析包含角質層的小鼠背部皮膚的阻抗特性。使用Agilent 4294A阻抗分析儀,測量麻醉狀態(tài)下的15只C57BL/6小鼠背部皮膚的電阻抗頻譜,使用表面電極和銀針電極,分別對應皮膚和活性皮膚層阻抗數(shù)據(jù)。在整個實驗頻率范圍(40~10~7Hz)內(nèi),隨頻率升高,皮膚阻抗幅值呈現(xiàn)-20dB/dec下降;活性皮膚層在40~10~3Hz之間幅值呈現(xiàn)-10 dB/dec下降,而在10~3~10~7Hz之間呈現(xiàn)-3dB/dec下降。皮膚相位隨頻率上升呈現(xiàn)V型曲線,而活性皮膚層相位呈現(xiàn)Π型曲線。低頻時,皮膚阻抗完全由角質層阻抗組成,活性皮膚層的貢獻可以忽略不計;當頻率為10~5Hz時,活性皮膚層阻抗占皮膚的10%多,而在10~7Hz以上時,活性皮膚層阻抗占整個皮膚的80%以上。皮膚阻抗與活性皮膚層阻抗在10~3Hz以下具有顯著性差異(P0.05),在10~4Hz以上無顯著性差異。結果表明,表面電極與銀針電極相結合的對比分析方法能夠在微創(chuàng)前提下有效分析角質層在皮膚阻抗中的作用。
[Abstract]:The great difference of impedance between cuticle and other layers makes it difficult to study the impedance characteristics of skin as a whole. To study the noninvasive or minimally invasive detection and analysis of the impedance characteristics of mouse dorsal skin containing cuticle in vivo. Agilent 4294A impedance analyzer was used to measure the electrical impedance spectrum of the back skin of 15 C57BL/6 mice under anesthesia. The surface electrode and silver needle electrode were used to correspond to the impedance data of the skin and the active skin layer, respectively. In the whole experimental frequency range (40~10~7Hz), the skin impedance amplitude decreased by -20 dB / dec, and the active skin layer decreased by -10 dB/dec between 40~10~3Hz and -3 dB / dec between 10~3~10~7Hz. The phase of the skin showed V shape curve with the frequency rising, while the phase of the active skin layer showed a Pi shape curve. At low frequency, the skin impedance is completely composed of the cuticle impedance, and the contribution of the active skin layer is negligible. When the frequency is 10~5Hz, the active skin layer impedance accounts for more than 10% of the skin, but above the 10~7Hz, The impedance of active skin layer accounted for more than 80% of the whole skin. There was significant difference between skin impedance and active skin layer impedance below 10~3Hz (P0.05), but there was no significant difference above 10~4Hz. The results show that the comparative analysis method of surface electrode and silver needle electrode can effectively analyze the role of cuticle in skin impedance on the premise of minimally invasive.
【作者單位】： 中國醫(yī)學科學院北京協(xié)和醫(yī)學院生物醫(yī)學工程研究所;英國卡迪夫大學生物醫(yī)學與生命科學學院口腔醫(yī)院;
【基金】：國家自然科學基金儀器專項(81127003);國家自然科學基金(81171424)
【分類號】：R318

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