草蓯蓉提取物對(duì)油酸型大鼠急性呼吸窘迫綜合征的保護(hù)作用
發(fā)布時(shí)間：2018-10-05 17:10
【摘要】：目的： 1.觀察不同劑量的草蓯蓉提取物對(duì)ARDS大鼠血清中TNF-α、IL-10水平的影響； 2.研究草蓯蓉提取物對(duì)ARDS大鼠肺組織中磷酸化的p38MAPK (p-p38MAPK)蛋白的表達(dá)； 3.探討草蓯蓉提取物對(duì)ARDS的保護(hù)作用。 方法： 將50只平均體重為(200±20)g的SD雄性大鼠適應(yīng)性飼養(yǎng)一周后隨機(jī)分為5組,每組10只,分別為空白組、模型組、草蓯蓉低劑量組、草蓯蓉中劑量組、草蓯蓉高劑量組。實(shí)驗(yàn)開始第1-6天每天按1ml/100g分別給空白組和模型組大鼠生理鹽水灌胃,同時(shí)給草蓯蓉低、中、高劑量3個(gè)組分別按125mg/kg、250mg/kg、500mg/kg劑量連續(xù)灌胃草蓯蓉提取物。在實(shí)驗(yàn)第6天將所有動(dòng)物行烏拉坦(1.0g/kg)腹腔麻醉后,空白組尾靜脈注射生理鹽水5ml/kg,模型組、草蓯蓉低劑量組、草蓯蓉中劑量組、草蓯蓉高劑量組尾靜脈注射油酸0.1ml/kg。3小時(shí)后,觀察大鼠一般狀態(tài),處死大鼠。左肺計(jì)算肺組織濕/干重(W/D)比值；用HE染色法觀察右肺組織病理變化；用ELISA法檢測(cè)血清中TNF-α和IL-10的濃度；用免疫印跡法檢測(cè)右肺組織中p-p38MAPK蛋白的表達(dá)。 結(jié)果： 1.與空白組比較,模型組大鼠左肺的W/D明顯升高(P0.05)；與模型組比較,草蓯蓉中、高劑量組大鼠左肺的W/D明顯降低(P0.05)。 2.組織病理學(xué)HE染色結(jié)果顯示：光鏡下,空白組大鼠肺組織結(jié)構(gòu)清晰,細(xì)支氣管粘膜上皮完整,假復(fù)層柱狀上皮細(xì)胞排列有序,肺泡腔內(nèi)清晰,間隔完整無水腫,無明顯炎性滲出；模型組大鼠肺組織結(jié)構(gòu)不完整,且肺泡萎陷,肺泡腔內(nèi)見以中性粒細(xì)胞為主的炎性細(xì)胞浸潤,以及大量滲出性蛋白水腫液,肺間質(zhì)毛細(xì)血管擴(kuò)張、充血,肺泡間隔明顯增厚、水腫；草蓯蓉各劑量組大鼠肺組織結(jié)構(gòu)基本完整,肺泡腔內(nèi)水腫、中性粒細(xì)胞浸潤以及肺泡間隔增厚較模型組減輕。草蓯蓉高劑量組炎性病變較低、中劑量組輕。 3.與空白組比較,模型組大鼠血清中的TNF-α、IL-10濃度明顯升高(P0.05)；與模型組比較,草蓯蓉中、高劑量組大鼠的TNF-α、IL-10濃度明顯降低(P0.05)。 4.空白組大鼠肺組織中p-p38MAPK蛋白的表達(dá)很弱,模型組中p-p38MAPK蛋白的表達(dá)明顯增加,草蓯蓉各劑量組中p-p38MAPK的表達(dá)較模型組明顯降低。 結(jié)論： 草蓯蓉提取物在ARDS發(fā)生時(shí)對(duì)正常肺組織有明顯的保護(hù)作用。 1.草蓯蓉提取物抑制血清中TNF-α、IL-10的表達(dá)； 2.草蓯蓉提取物抑制肺組織中p-p38MAPK的表達(dá),其原因可能與草蓯蓉對(duì)MAPK通路的調(diào)節(jié)有關(guān)； 3.草蓯蓉提取物為125-500mg/kg劑量之間時(shí),在ARDS發(fā)生時(shí)對(duì)正常肺組織的保護(hù)作用與劑量關(guān)系成正比。
[Abstract]:Objective: 1. To observe the effect of different doses of Cistanche deserticola extract on the level of TNF- 偽 -IL-10 in serum of ARDS rats. To study the effect of Cistanche deserticola extract on the expression of phosphorylated p38MAPK (p-p38MAPK) protein in lung tissue of ARDS rats. To investigate the protective effect of Cistanche deserticola extract on ARDS. Methods: fifty male SD rats with average body weight of (200 鹵20) g were randomly divided into 5 groups, 10 rats in each group, which were blank group, model group, Cistanche deserticola low dose group and middle dose group. High dose group of Cistanche deserticola. At the beginning of 1-6 days, the rats in the control group and the model group were given 1ml/100g daily with normal saline, while Cistanche deserticola was administered with low, medium and high doses of 125 mg / kg 250 mg / kg ~ 500 mg / kg Cistanche deserticola extract respectively by intragastric administration of Cistanche deserticola extract at the dose of 125 mg / kg ~ 250 mg 路kg ~ (-1) 路kg ~ (-1) ~ (-1). On the 6th day of the experiment, all the animals were anesthetized intraperitoneally with 1.0g/kg, the rats in the blank group were injected with saline 5 ml / kg via tail vein, the model group, the low dose group of Cistanche deserticola, the middle dose group of Cistanche deserticola, and the high dose group of Cistanche deserticola were injected with oleic acid 0.1ml/kg.3 hours later. The general state of the rats was observed and the rats were killed. The wet / dry weight (W / D) ratio of the left lung was calculated, the pathological changes of the right lung were observed by HE staining, the serum TNF- 偽 and IL-10 levels were detected by ELISA method, and the expression of p-p38MAPK protein in the right lung was detected by Western blotting. Results: 1. Compared with the blank group, the W / D of the left lung in the model group was significantly higher than that in the model group (P0.05); compared with the model group, the W / D of the left lung in the high dose group was significantly lower than that in the model group (P0.05). 2. The results of histopathological HE staining showed that the lung tissue structure was clear, the bronchiolar mucosal epithelium was intact, the pseudostratified columnar epithelial cells were arranged orderly, the alveolar cavity was clear and the septum was intact without edema. There was no obvious inflammatory exudation in the model group, the pulmonary tissue structure was incomplete and alveolar collapse was found in the model group, the infiltration of inflammatory cells with neutrophil in the alveolar cavity, as well as a large amount of exudative protein edema fluid, pulmonary interstitial capillary dilatation and hyperemia were observed in the alveolar cavity. The alveolar septum was thickened and edema, the lung tissue structure of Cistanche deserticola group was basically intact, the alveolar cavity edema, neutrophil infiltration and alveolar septal thickening were less than those in the model group. The inflammatory lesion of Cistanche deserticola in high dose group was lower than that in middle dose group. 3. Compared with the blank group, the serum TNF- 偽 -IL-10 concentration in the model group was significantly higher (P0.05); compared with the model group, the concentration of TNF- 偽 -IL-10 in the Cistanche deserticola group was significantly lower than that in the high dose group (P0.05). 4. The expression of p-p38MAPK protein in lung tissue of blank group was very weak, the expression of p-p38MAPK protein in model group was obviously increased, and the expression of p-p38MAPK in each dose group of Cistanche deserticola was significantly lower than that in model group. Conclusion: Cistanche deserticola extract has obvious protective effect on normal lung tissue during ARDS. 1. The extract of Cistanche deserticola inhibited the expression of TNF- 偽 -IL-10 in serum. The extract of Cistanche deserticola inhibited the expression of p-p38MAPK in lung tissue, which may be related to the regulation of MAPK pathway by Cistanche deserticola. 3. The protective effect of Cistanche deserticola extract on normal lung tissue was proportional to the dose when the dose of Cistanche deserticola was between the doses of 125-500mg/kg.
【學(xué)位授予單位】：延邊大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R285.5
本文編號(hào)：2254189
[Abstract]:Objective: 1. To observe the effect of different doses of Cistanche deserticola extract on the level of TNF- 偽 -IL-10 in serum of ARDS rats. To study the effect of Cistanche deserticola extract on the expression of phosphorylated p38MAPK (p-p38MAPK) protein in lung tissue of ARDS rats. To investigate the protective effect of Cistanche deserticola extract on ARDS. Methods: fifty male SD rats with average body weight of (200 鹵20) g were randomly divided into 5 groups, 10 rats in each group, which were blank group, model group, Cistanche deserticola low dose group and middle dose group. High dose group of Cistanche deserticola. At the beginning of 1-6 days, the rats in the control group and the model group were given 1ml/100g daily with normal saline, while Cistanche deserticola was administered with low, medium and high doses of 125 mg / kg 250 mg / kg ~ 500 mg / kg Cistanche deserticola extract respectively by intragastric administration of Cistanche deserticola extract at the dose of 125 mg / kg ~ 250 mg 路kg ~ (-1) 路kg ~ (-1) ~ (-1). On the 6th day of the experiment, all the animals were anesthetized intraperitoneally with 1.0g/kg, the rats in the blank group were injected with saline 5 ml / kg via tail vein, the model group, the low dose group of Cistanche deserticola, the middle dose group of Cistanche deserticola, and the high dose group of Cistanche deserticola were injected with oleic acid 0.1ml/kg.3 hours later. The general state of the rats was observed and the rats were killed. The wet / dry weight (W / D) ratio of the left lung was calculated, the pathological changes of the right lung were observed by HE staining, the serum TNF- 偽 and IL-10 levels were detected by ELISA method, and the expression of p-p38MAPK protein in the right lung was detected by Western blotting. Results: 1. Compared with the blank group, the W / D of the left lung in the model group was significantly higher than that in the model group (P0.05); compared with the model group, the W / D of the left lung in the high dose group was significantly lower than that in the model group (P0.05). 2. The results of histopathological HE staining showed that the lung tissue structure was clear, the bronchiolar mucosal epithelium was intact, the pseudostratified columnar epithelial cells were arranged orderly, the alveolar cavity was clear and the septum was intact without edema. There was no obvious inflammatory exudation in the model group, the pulmonary tissue structure was incomplete and alveolar collapse was found in the model group, the infiltration of inflammatory cells with neutrophil in the alveolar cavity, as well as a large amount of exudative protein edema fluid, pulmonary interstitial capillary dilatation and hyperemia were observed in the alveolar cavity. The alveolar septum was thickened and edema, the lung tissue structure of Cistanche deserticola group was basically intact, the alveolar cavity edema, neutrophil infiltration and alveolar septal thickening were less than those in the model group. The inflammatory lesion of Cistanche deserticola in high dose group was lower than that in middle dose group. 3. Compared with the blank group, the serum TNF- 偽 -IL-10 concentration in the model group was significantly higher (P0.05); compared with the model group, the concentration of TNF- 偽 -IL-10 in the Cistanche deserticola group was significantly lower than that in the high dose group (P0.05). 4. The expression of p-p38MAPK protein in lung tissue of blank group was very weak, the expression of p-p38MAPK protein in model group was obviously increased, and the expression of p-p38MAPK in each dose group of Cistanche deserticola was significantly lower than that in model group. Conclusion: Cistanche deserticola extract has obvious protective effect on normal lung tissue during ARDS. 1. The extract of Cistanche deserticola inhibited the expression of TNF- 偽 -IL-10 in serum. The extract of Cistanche deserticola inhibited the expression of p-p38MAPK in lung tissue, which may be related to the regulation of MAPK pathway by Cistanche deserticola. 3. The protective effect of Cistanche deserticola extract on normal lung tissue was proportional to the dose when the dose of Cistanche deserticola was between the doses of 125-500mg/kg.
【學(xué)位授予單位】：延邊大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R285.5
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