【摘要】：目的：探討七氟烷預處理是否可以保護大鼠肺缺血再灌注損傷及其作用機制,從而為臨床麻醉實施中麻醉藥物的選擇提供實驗和理論依據(jù)。方法：成年雄性SD大鼠54只,體重為250-320g左右,采用隨機數(shù)字表法,將其隨機分為3組(n=18)假手術組(S組)：僅游離左肺門,但不阻斷；肺缺血再灌注組(I/R組)：采用阻斷左肺門45min后再恢復灌注的方法制備大鼠肺缺血再灌注損傷模型；七氟烷預處理組(SP組)：吸入濃度為2.1%的七氟烷30min后制備肺缺血再灌注模型。各組分別于再灌注30、60和120min時隨機取6只大鼠,處死后取肺組織,測定其濕/干重比值(W/D比),采用比色法測定肺組織髓過氧化物酶(MPO)活性,采用RT-PCR測肺組織ACE mRNA的表達水平,光鏡下觀察肺組織病理變化。結果：與S組比較,I/R組和SP組再灌注各時間點肺組織W/D比、MPO活性和ACE mRNA表達水平升高,差異具有統(tǒng)計學意義(P0.05)；I/R組和SP組隨著再灌注時間的延長肺組織W/D比、MPO活性、ACE mRNA表達水平增高,但在每個再灌注時間點,SP組的肺組織W/D比、MPO活性和ACE mRNA表達水平明顯低于I/R組(P0.05)；SP組的肺組織損傷較I/R組減輕。結論：七氟烷預處理可能通過下調(diào)ACE mRNA的表達和抑制MPO活性從而減輕肺缺血再灌注損傷。
[Abstract]:Aim: to investigate whether sevoflurane pretreatment can protect lung ischemia-reperfusion injury and its mechanism in rats, and provide experimental and theoretical basis for the selection of anesthetic drugs in clinical anesthesia. Methods: 54 adult male SD rats, weighing about 250-320g, were randomly divided into 3 groups (n = 18): sham operation group (S group): only left hilum was free, but not blocked. Lung ischemia-reperfusion group (I / R group): the model of lung ischemia-reperfusion injury was established by blocking left pulmonary hilus 45min after reperfusion, and that of sevoflurane preconditioning group (SP group) was induced by inhalation of 2.1% sevoflurane 30min. In each group, 6 rats were randomly selected during reperfusion (30 ~ 60) and 120min. Lung tissues were killed and their wet / dry weight ratio (W / D) was measured. The activity of myeloperoxidase (MPO) in lung tissue was measured by colorimetric method, and the expression of ACE mRNA in lung tissue was measured by RT-PCR. The pathological changes of lung tissue were observed under light microscope. Results: compared with S group, the lung tissue WR activity and ACE mRNA expression level of I / R group and SP group were significantly higher than those of SP group (P0.05). The expression of W- / D and ACE mRNA in lung tissue of group I / R and SP were significantly lower than that of group I / R (P0.05) with the prolongation of reperfusion time, but the expression of W- / D was significantly lower than that of group I / R at each time point after reperfusion (P < 0.05), but the expression of W- / D was significantly lower than that of group I / R (P < 0.05). Lung tissue injury in SP group was lighter than that in I / R group. Conclusion: sevoflurane preconditioning may reduce lung ischemia-reperfusion injury by down-regulating the expression of ACE mRNA and inhibiting the activity of MPO.
【學位授予單位】：新疆醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R614

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