【摘要】：目的 :探討麻醉類藥物氯胺酮和嗎啡對(duì)人肺癌A549細(xì)胞生長的影響及相關(guān)作用機(jī)制。方法:體外培養(yǎng)肺癌細(xì)胞系A(chǔ)549,細(xì)胞培養(yǎng)24 h后,隨機(jī)分為3組:對(duì)照組(NC組,加0.9%Na Cl處理)、氯胺酮組(K1、K2、K3組,分別加氯胺酮100、200、400μg/m L處理)和嗎啡組(M1、M2、M3組,分別加嗎啡25、50、100μg/m L處理)。持續(xù)作用24 h后,Transwell小室培養(yǎng)體系測定腫瘤細(xì)胞的遷移率;雙熒光素酶報(bào)告基因法測定NF-κB轉(zhuǎn)錄活性;48 h后MTT法測定細(xì)胞增殖抑制率;Western blot技術(shù)測定NF-κB P65的蛋白表達(dá);QPCR技術(shù)檢測細(xì)胞內(nèi)IL-1β和COX-2的m RNA表達(dá)。結(jié)果:1與NC組比較,M1、M2組表現(xiàn)為促進(jìn)A549細(xì)胞遷移(P0.05),M3組作用不明顯;K1、K2、K3組表現(xiàn)為抑制A549細(xì)胞遷移(P0.05);2與NC組比較,M1、M2組表現(xiàn)為促進(jìn)A549細(xì)胞增殖(P0.05),M3組作用不明顯;K2、K3組表現(xiàn)為抑制A549細(xì)胞增殖(P0.05),K1組不明顯;3與NC組比較,M1、M2、M3組表現(xiàn)為促進(jìn)IL-1β、COX-2的m RNA表達(dá)(P0.05);K2、K3組表現(xiàn)為抑制IL-1β、COX-2的m RNA表達(dá)(P0.05),K1組不明顯;4與NC組比較,M1、M2、M3組表現(xiàn)為促進(jìn)P65的轉(zhuǎn)錄和蛋白表達(dá)(P0.05),K1、K2、K3組表現(xiàn)為抑制P65的轉(zhuǎn)錄和蛋白表達(dá)(P0.05)。結(jié)論:與阿片類藥物不同,氯胺酮通過NF-κB信號(hào)通路及其下游因子IL-1β和COX-2抑制人肺腺癌細(xì)胞A549的遷移和增殖,可能更適用于肺癌患者的術(shù)后鎮(zhèn)痛。
[Abstract]:Aim: to investigate the effect of ketamine and morphine on the growth of human lung cancer cell line A549. Methods: lung cancer cell line A549 was cultured in vitro for 24 hours. After 24 h culture, the cells were randomly divided into three groups: control group (NC group, treated with 0.9%Na Cl), ketamine group (K1K2K2K3 group, treated with ketamine 100200400 渭 g / mL) and morphine group (M1m2M3 group, treated with morphine 2550100 渭 g / mL, respectively). The migration rate of tumor cells was measured in transwell chamber culture system after 24 hours of continuous treatment. Double luciferase reporter gene assay for NF- 魏 B transcriptional activity 48 h later, the inhibitory rate of cell proliferation was determined by MTT assay. Protein expression of NF- 魏 B p65 was detected by Western blot. The expression of IL-1 尾 and m RNA in cells was detected by QPCR. Results compared with NC group, the effect of M1M 2 group was to promote the migration of A549 cells (P0.05) the effect of M3 group was to inhibit the migration of A549 cells (P0.05). Compared with the NC group, the M1M 2 group was able to promote the proliferation of A549 cells (P0.05), the effect of M3 group was not obvious, and the K2K3 group showed no obvious inhibitory effect on the proliferation of A549 cells. Proliferation of A549 cells (P0.05) the expression of m RNA of IL-1 尾 -COX-2 in M1M2M3 group was significantly higher than that in NC group (P0.05). (P0.05) the expression of m RNA of IL-1 尾 -COX-2 was inhibited in K2K3 group (P0.05). 4 the expression of p65 transcription and protein in M1M2M3 group was not obvious compared with that in NC group (P0.05). (P 0.05) the expression of p65 transcription and protein was inhibited in K1 K2K3 group (P0.05). Conclusion: ketamine inhibits the migration and proliferation of human lung adenocarcinoma cell line A549 through NF- 魏 B signaling pathway and its downstream factors IL-1 尾 and COX-2, which may be more suitable for postoperative analgesia in patients with lung cancer.
【作者單位】： 南通大學(xué)附屬腫瘤醫(yī)院麻醉科;南京醫(yī)科大學(xué)公共衛(wèi)生學(xué)院;
【基金】：南通市市級(jí)科技計(jì)劃(指導(dǎo)性,HS13928)
【分類號(hào)】：R734.2

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