【摘要】：目的：運用ERPs技術(shù)，觀察電針手少陰心經(jīng)原穴“神門”與電針手太陰肺經(jīng)原穴“太淵”對急性心肌缺血家兔事件相關(guān)電位P50和MMN幅值與潛伏期的影響，比較“神門”和“太淵”穴對大腦感覺門控能力（P50）、信息自動加工（MMN）影響的特異性，為明確針刺效應(yīng)與腦聯(lián)系的機制提供新的實驗依據(jù)。 方法：選用成年健康青紫蘭家兔50只，雌雄不拘，分籠飼養(yǎng)，體質(zhì)量(2.5±0.5)kg，，隨機選10只作正常對照組，其余家兔在麻醉狀態(tài)下（8%水合氯醛，1.8ml/kg），采用左冠狀動脈前降支結(jié)扎法復(fù)制急性心肌缺血家兔模型，并將模型復(fù)制成功的家兔隨機分為模型組、電針“神門”組和電針“太淵”組，每組10只。各電針組刺激電流為1mA,頻率為2Hz,每次刺激15min,進行電針治療。電針結(jié)束后，利用美國NEUROSCANERPs工作站，其中E-prime2.0作為刺激器，采用Scan4.3記錄并對記錄的數(shù)據(jù)進行處理分析。采用Spass17.0forWindows進行數(shù)據(jù)統(tǒng)計分析，比較各組家兔大腦皮層聽感覺門控電位P50、失匹配負波MMN各指標(biāo)的變化情況。 結(jié)果： （1）Powerlab心電圖：與正常組比較，造模后家兔心率顯著性下降，ST段顯著性抬高,T波倒置。 （2）感覺門控電位P50：各組S1-P50潛伏期和幅值、S2-P50潛伏期比較均無差異（P0.05）。與正常組比較，模型組S2-P50幅值、S2/S1增大（均P＜0.01），S1-S2、100*(1-S2/S1)減�。ň鵓＜0.01）；與模型組比較，電針“神門”組家兔S2-P50幅值、S2/S1減�。ň鵓＜0.01），S1-S2、100*(1-S2/S1)增大（均P＜0.01），電針“太淵”組無差異（均P0.05）�！吧耖T”組與“太淵”組之間S2-P50幅值、S2/S1、S1-S2、100*(1-S2/S1)差異有統(tǒng)計學(xué)意義（P＜0.01）。 （3）失匹配負波（MismatchnegativityMMN）:與正常組比，模型組潛伏期延長，幅值減小（均P＜0.01）；與模型組比，“神門”組潛伏期縮短、幅值升高（均P＜0.01），“太淵”組比較無統(tǒng)計學(xué)意義（均P0.05）；“神門”組與“太淵”組之間的差異具有統(tǒng)計學(xué)意義（P＜0.01）。 結(jié)論： （1）急性心肌缺血狀態(tài)下的家兔大腦感覺門控（P50）、信息自動加工能力（MMN）存在異常變化。 （2）電針不同經(jīng)穴對急性心肌缺血家兔大腦皮層感覺門控的調(diào)整作用有相對特異性，電針心經(jīng)原穴“神門”的調(diào)整作用優(yōu)于電針肺經(jīng)原穴“太淵”。 （3）電針不同經(jīng)穴對急性心肌缺血家兔大腦信息自動加工能力的影響具有特異性，電針心經(jīng)原穴“神門”的調(diào)整作用優(yōu)于電針肺經(jīng)原穴“太淵”。
[Abstract]:Objective: to observe the effects of electroacupuncture on the event-related potential P50, MMN amplitude and latency of acute myocardial ischemia in rabbits with acute myocardial ischemia by electroacupuncture at Shenmen, the original point of Shaoyin and Xinjing, and Taiyuan, which is the original point of Taiyin lung meridian. The effects of "Shenmen" and "Taiyuan" on brain sensory gating ability (P50) and automatic processing of (MMN) were compared, which provided a new experimental basis for clarifying the mechanism of acupuncture effect and brain connection. Methods: 50 adult healthy blue rabbits, male and female, were raised in cage, and the body weight was (2.5 鹵0.5) kg. 10 rabbits were randomly selected as normal control group. Under anesthesia (8% chloral hydrate 1.8ml / kg), the rabbit model of acute myocardial ischemia was established by ligating the anterior descending branch of left coronary artery. The rabbits were randomly divided into three groups: model group, electroacupuncture "Shenmen" group and electroacupuncture "Taiyuan" group. 10 rats in each group. The stimulation current of each electroacupuncture group was 1 Ma and the frequency was 2 Hz. At the end of electroacupuncture, the American NEUROSCANERPs workstation was used, in which E-prime2.0 was used as stimulator, Scan4.3 was used to record and process the recorded data. To compare the changes of auditory sensory gated potential (P50) and mismatched negative wave (MMN) in the cerebral cortex of rabbits with Spass17.0forWindows. Results: (1) Powerlab electrocardiogram: compared with the normal group, there was no significant difference in the latency of S1-P50 and the amplitude of S2-P50 (P0.05) after the establishment of the model, the significant decrease of heart rate and the elevation of T wave inversion of St segment in the model group. (2) the sensory gated potential P50: there was no difference in the latency of S1-P50 and the amplitude of S2-P50 in each group (P0.05). Compared with the normal group, the amplitude of S2-P50 in the model group increased (P < 0. 01) and the S1-S2100 * (1-S2/S1) decreased (all P < 0. 01), and the amplitude of S2-P50 in the "Shenmen" group decreased (all P < 0. 01), the value of S1-S2100 * (1-S2/S1) increased (all P < 0. 01), but there was no difference in the electroacupuncture "Taiyuan" group (P0.05). There was a significant difference in S2-P50 amplitude between "Shenmen" group and "Taiyuan" group (1-S2/S1) (P < 0. 01). (3). Compared with normal group, the latent period of model group was prolonged and the amplitude decreased (P < 0. 01), while that of model group was higher than that of model group. The latent period of "Shenmen" group was shortened and the amplitude was increased (all P < 0.01), but there was no significant difference between "Taiyuan" group and "Shenmen" group (P0.05), and the difference between "Shenmen" group and "Taiyuan" group was statistically significant (P < 0.01). Conclusion: (1) there are abnormal changes in sensory gating (P50) and information automatic processing ability (MMN) in the brain of rabbits with acute myocardial ischemia. (2) electroacupuncture at different acupoints can affect the sensory function of cerebral cortex in rabbits with acute myocardial ischemia. The regulation of gating has relative specificity. The regulating effect of electroacupuncture on the original acupoint "Shenmen" of the heart meridian was better than that on the "Taiyuan" of the original point of the electroacupuncture of the lung meridian. (3) the effect of different acupoints of electroacupuncture on the ability of automatic processing of cerebral information in rabbits with acute myocardial ischemia was specific. The regulating effect of the original acupoint Shenmen of electroacupuncture is superior to that of the original acupoint Taiyuan of the lung meridian of electroacupuncture.
【學(xué)位授予單位】：安徽中醫(yī)藥大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R245

【參考文獻】

相關(guān)期刊論文 前10條

1 周美啟,周逸平;“心主神明”探要[J];安徽中醫(yī)學(xué)院學(xué)報;2004年06期

2 周美啟;周逸平;汪克明;胡玲;沈曉明;王月蘭;陳業(yè)農(nóng);;針刺心經(jīng)、小腸經(jīng)干預(yù)急性心肌缺血下丘腦基因表達[J];安徽中醫(yī)學(xué)院學(xué)報;2007年02期

3 周逸平;周美啟;汪克明;胡玲;吳子建;王月蘭;陳業(yè)農(nóng);;經(jīng)脈臟腑與腦相關(guān)研究是中西醫(yī)理論結(jié)合的突破口[J];安徽中醫(yī)學(xué)院學(xué)報;2008年01期

4 梁宏;崔俊峰;王宇航;;針?biāo)幗Y(jié)合治療冠心病心絞痛臨床觀察[J];長春中醫(yī)藥大學(xué)學(xué)報;2011年05期

5 林芳;徐珊寧;;溫和灸加淺針治療心肌缺血60例[J];福建中醫(yī)學(xué)院學(xué)報;2008年01期

6 孫忠人;王一茗;張小軍;;針刺預(yù)處理對心肌缺血大鼠HSP70的影響[J];甘肅中醫(yī);2008年08期

7 閆海龍;劉元峰;宋盼;;穴位注射治療穩(wěn)定型心絞痛64例[J];廣西中醫(yī)藥;2011年05期

8 張慈祿,王國珍,邱彩花,蔡維國,應(yīng)淵;心臟病人的心理改變研究[J];國醫(yī)論壇;1996年03期

9 林婉娟;陳東風(fēng);張少君;;針灸治療冠心病心絞痛30例臨床觀察[J];中醫(yī)藥導(dǎo)報;2008年06期

10 吳澤銘;邢潔;張大創(chuàng);鐘玉霞;張桂燕;周晃如;;通心貼外敷心俞穴對冠心病心絞痛NO、NOS、SOD、MDA的影響[J];河南中醫(yī)學(xué)院學(xué)報;2007年04期

本文編號：2186086