【摘要】：目的觀察海馬內(nèi)微清蛋白(PV)表達(dá)變化在老年小鼠術(shù)后認(rèn)知功能障礙(POCD)中的作用,并探討磷酸酰胺腺嘌呤二核苷酸(NADPH)氧化酶是否參與其中。方法采用異氟醚麻醉+剖腹探查術(shù)建立POCD動(dòng)物模型。雄性16月齡C57BL/6小鼠21只,隨機(jī)均分為三組(n=7):對(duì)照+溶劑組(C+V組)、異氟醚麻醉+剖腹探查+溶劑組(POCD+V組)、異氟醚麻醉+剖腹探查+夾竹桃麻素組(POCD+A組)。POCD+A組術(shù)后1h腹腔注射夾竹桃麻素5mg/kg,其后每天注射一次直至術(shù)后第7天;其余兩組注射等容量的溶劑。術(shù)后第6天行曠場(chǎng)實(shí)驗(yàn)和條件性恐懼實(shí)驗(yàn)訓(xùn)練,第7天在給予藥物或溶劑后2h行條件性恐懼測(cè)試。行為學(xué)測(cè)試后2h取小鼠海馬組織,采用Western blot檢測(cè)PV、gp91phox、p22phox及羥基壬烯酸(4-HNE)的表達(dá)變化。結(jié)果三組小鼠總探索路程和中央格停留時(shí)間差異無(wú)統(tǒng)計(jì)學(xué)意義。與C+V組比較,POCD+V組在場(chǎng)景性條件恐懼測(cè)試中僵直時(shí)間明顯縮短,海馬PV表達(dá)明顯下降,而gp91phox、p22phox和4-HNE表達(dá)明顯升高(P0.05);與POCD+V組比較,POCD+A組在場(chǎng)景性條件恐懼測(cè)試中僵直時(shí)間明顯延長(zhǎng),海馬PV表達(dá)明顯升高,gp91phox、p22phox和4-HNE表達(dá)明顯降低(P0.05)。結(jié)論海馬NADPH氧化酶活化介導(dǎo)PV表達(dá)降低可能參與老年小鼠POCD。
[Abstract]:Objective to investigate the role of microalbumin (PV) expression in postoperatively aged mice with cognitive dysfunction (POCD), and to explore whether (NADPH) oxidase of adenine dinucleotide is involved in it. Methods POCD animal model was established by isoflurane anesthesia. Twenty-one male 16-month-old C57BL/6 mice, They were randomly divided into three groups: control group (C V group), isoflurane anesthesia group (POCD V group), isoflurane anesthesia group (POCD A group). After 5 mg / kg, the rats were injected once a day until the 7th day after operation. The other two groups were injected with the same volume of solvent. The open field test and conditioned fear test were performed on the 6th day after operation, and the conditioned fear test was performed on the 7th day at 2 hours after the administration of the drug or solvent. Western blot was used to detect the expression of PVGp91phoxP22phox and hydroxylazenoic acid (4-HNE) in hippocampus of mice 2 h after behavioral test. Results there was no significant difference in total exploration distance and residence time between the three groups. Compared with C V group, POCD V group had significantly shorter stiffness time and lower hippocampal PV expression. Compared with the POCD V group, the expression of gp91phoxtrophox p22phox and 4-HNE was significantly increased (P0.05). Compared with the POCD V group, the rigidity time was significantly prolonged and the expression of p22phox and 4-HNE in hippocampus was significantly increased (P0.05). Conclusion the decrease of PV expression mediated by NADPH oxidase activation in hippocampus may be involved in POCD expression in aged mice.
【作者單位】： 南京大學(xué)醫(yī)學(xué)院臨床學(xué)院南京軍區(qū)南京總醫(yī)院麻醉科;
【基金】：國(guó)家自然科學(xué)基金(81271216,81300946)
【分類(lèi)號(hào)】：R614

【共引文獻(xiàn)】

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