【摘要】：目的:通過建立潰瘍性結(jié)腸炎(Ulcerative colitis,UC)大鼠模型,觀察魚腥草聯(lián)合龍血竭對UC大鼠血清白細(xì)胞介素10(Inerleukin-10,IL-10)、腫瘤壞死因子(Tumor Necrosis Factor-α,TNF-α)、血小板活化因子(Platelet-Activating Factor,PAF)的影響,驗證魚腥草及龍血竭對UC的治療作用,探討二者聯(lián)合應(yīng)用是否對UC的治療有協(xié)同作用及其作用機(jī)理,從而為UC規(guī)范化治療及用藥提供依據(jù)。方法:將96只雌雄各半的SD大鼠適應(yīng)性喂養(yǎng)1周后,隨機(jī)抽取12只為空白組,84只為造模組。造模前,大鼠全部禁食不禁飲24h,自由飲水。予三硝基苯磺酸(TNBS)/乙醇溶液灌腸造模成功后,將造模組的大鼠隨機(jī)分為7組,即模型組、柳氮磺吡啶(SASP)組、魚腥草合龍血竭低劑量組、魚腥草合龍血竭中劑量組、魚腥草合龍血竭高劑量組、魚腥草組、龍血竭組。每組分別給予相應(yīng)的藥物灌胃,3ml/次,1次/日,共14天。給藥期間觀察各組大鼠的一般情況、體重變化及糞便隱血實驗,最后一次給藥結(jié)束后禁食不禁飲24h,腹腔麻醉下心臟采血,檢測大鼠血清IL-10、PAF、TNF-α水平,采血結(jié)束后頸椎脫位法處死大鼠,取結(jié)腸組織觀察各組結(jié)腸粘膜變化情況,并做病理組織學(xué)檢查。結(jié)果:魚腥草合龍血竭高劑量組較魚腥草合龍血竭中劑量組、SASP組、龍血竭組、魚腥草組、魚腥草合龍血竭低劑量組、模型組依次一般狀態(tài)恢復(fù)快,膿血便消失早,中劑量組與SASP組療效相近。聯(lián)合用藥組、SASP組、龍血竭組、魚腥草組均表現(xiàn)為腸粘膜組織修復(fù)明顯,充血水腫,糜爛和潰瘍基本愈合。UC大鼠血清中IL-10含量較空白組明顯降低,TNF-α、PAF明顯升高(P0.01);藥物灌胃后,魚腥草聯(lián)合龍血竭高劑量組中IL-10含量明顯升高,TNF-α、PAF明顯降低,與模型組、SASP組、魚腥草聯(lián)合龍血竭低、中劑量組、魚腥草組及龍血竭組相比,差異具有顯著性(P0.01);魚腥草聯(lián)合龍血竭中、低劑量組、魚腥草組、龍血竭組、SASP組中IL-10含量亦有升高,TNF-α、PAF也有所降低,與模型組相比有顯著性差異(P0.01);SASP組血清IL-10、TNF-α、PAF與魚腥草聯(lián)合龍血竭中劑量組、魚腥草組、龍血竭組比較,無顯著性差異(P0.05),但與魚腥草聯(lián)合龍血竭低劑量組相比,差異具有統(tǒng)計學(xué)意義(P0.05);魚腥草聯(lián)合龍血竭低劑量組血清IL-10、TNF-α、PAF與中、高劑量組相比,差異具有統(tǒng)計學(xué)意義(P0.05),但與魚腥草組、龍血竭組相比,差異無統(tǒng)計學(xué)意義(P0.05)。結(jié)論:1.魚腥草、龍血竭對UC大鼠模型有治療作用,二藥聯(lián)合用藥對UC的治療具有協(xié)同作用,高劑量組尤甚且療效優(yōu)于SASP。2.魚腥草聯(lián)合龍血竭治療UC的可能機(jī)制:(1)上調(diào)抗炎因子IL-10,從而抑制促炎因子,調(diào)節(jié)腸道細(xì)胞平衡,維持正常腸道粘膜免疫調(diào)節(jié)。(2)下調(diào)PAF,改善血小板激活狀態(tài),促進(jìn)組織修復(fù)。下調(diào)促炎因子TNF-α,減輕結(jié)腸組織的急性炎癥反應(yīng),阻斷UC病變中肉芽腫的形成和纖維組織增生,促進(jìn)潰瘍愈合。
[Abstract]:Objective: to observe the effects of Houttuynia cordata combined with Dragon blood on serum interleukin-10 (IL-10), tumor necrosis factor (Tumor Necrosis Factor- 偽 (TNF- 偽), and platelet activating factor (Platelet-Activating) in UC rats by establishing a rat model of ulcerative colitis (UC). To verify the therapeutic effect of Houttuynia cordata and Dragon Blood Draconis on UC, and to explore whether the combined use of Houttuynia cordata and Draconis has synergistic effect on UC and its mechanism, so as to provide the basis for the standardized treatment and medication of UC. Methods: 96 male and female Sprague-Dawley rats were fed adaptively for one week, and 12 SD rats were randomly selected as blank group (n = 84) and model group (n = 84). Before the model was made, all rats fasted and drank freely for 24 hours. After successful enema with (TNBS) / ethanol solution of trinitrobenzenesulfonic acid, the rats in the model group were randomly divided into 7 groups: model group, (SASP) group, low dose group of houttuynia houttuyniae, middle dose group of houttuynia houttuyniae, and middle dose group of houttuynia houttuyniae. Houttuynia cordata Houttuyniae Houttuynia houttuyniae high dose group, houttuynia group, dragon blood group. Each group was given 3 ml / d intragastric administration of corresponding drugs for 14 days. During the course of administration, the changes of body weight and fecal occult blood test were observed in each group. After the last administration, the rats could not stop drinking for 24 hours. The blood was taken from the heart under abdominal anesthesia, and the level of serum IL-10 and PAFNF-TNF- 偽 was detected. The colonic tissue was taken to observe the changes of colonic mucosa and histopathological examination. Results: the high dose group of Houttuynia cordata Houttuynia houttuyniae was faster than that of the middle dose group of Houttuynia houttuyniae in SASP group, the Dragon blood group and the low dose group of Houttuynia houttuyniae. The general state of the model group recovered faster, and the abscess disappeared earlier than that in the model group. The effect of middle dose group was similar to that of SASP group. The results showed that the intestinal mucosal tissue repair was obvious, hyperemia and edema, and the content of IL-10 in the serum of UC rats were significantly lower than those of the blank group (P0.01), and the contents of IL-10 in the serum of the rats were significantly lower than those of the blank group (P0.01), and the contents of IL-10 in the serum of rats with hyperemia and ulcer healing were significantly lower than those of the control group (P0.01). The content of IL-10 in Houttuynia cordata combined with high dose group was significantly higher than that in model group, Houttuynia houttuyniae combined with dragon blood group, middle dose group, Houttuynia cordata group and dragon blood group, compared with model group, Houttuynia cordata combined with dragon blood group. The difference was significant (P0.01), the content of IL-10 in the low dose group, the houttuynia group, and the dragon blood group also increased, and the content of TNF- 偽 was also decreased. There was no significant difference in serum IL-10 TNF- 偽 PAF between SASP group and Houttuynia cordata group, middle dose group, houttuynia group and dragon blood group (P0.05), but there was no significant difference compared with the low dose group of Houttuynia cordata combined with dragon blood exhaustion (P 0.05). The difference was statistically significant (P0.05); the serum IL-10 TNF- 偽 PAF in low dose group was significantly higher than that in high dose group (P0.05), but there was no significant difference compared with Houttuynia group and Dragon blood group (P0.05). Conclusion 1. Houttuynia cordata and Dragon Blood Draconis have therapeutic effect on UC rat model. The combination of two drugs has synergistic effect on UC, especially in the high dose group and the curative effect is better than that of SASP.2. The possible mechanisms of Houttuynia cordata combined with Dragon Blood in the treatment of UC were as follows: (1) upregulation of anti-inflammatory factor IL-10, thus inhibition of pro-inflammatory factor, regulation of intestinal cell balance, maintenance of normal intestinal mucosal immune regulation, (2) down-regulation of PAF, improvement of platelet activation, and promotion of tissue repair. The inflammatory factor TNF- 偽 was down-regulated, the acute inflammatory reaction of colonic tissue was alleviated, granuloma formation and fibrous tissue proliferation in UC were blocked, and ulcer healing was promoted.
【學(xué)位授予單位】：川北醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R574.62

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 何山;劉凱;黃永坤;;美沙拉秦、酪酸梭菌、蒙脫石散對UC大鼠血中PG-E2、LT-B4、PAF、VEGF的影響[J];中國免疫學(xué)雜志;2016年08期

2 郭易娟;梅浙川;;炎癥性腸病血液高凝狀態(tài)的研究進(jìn)展[J];重慶醫(yī)學(xué);2016年05期

3 趙平;董蕾;羅金燕;管海濤;宋亞華;龔均;;DSS與TNBS誘導(dǎo)大鼠實驗性結(jié)腸炎模型的對比研究[J];胃腸病學(xué);2015年11期

4 竇紅宇;孫濱濱;丁珂;楊雪春;李曉光;李建平;;美沙拉嗪口服聯(lián)合龍血竭散灌腸治療潰瘍性結(jié)腸炎療效及對患者生活質(zhì)量的影響[J];中國現(xiàn)代醫(yī)學(xué)雜志;2014年34期

5 宋莎莎;楊樂;俞令凱;林陳石;胡婭;;合成魚腥草素對潰瘍性結(jié)腸炎小鼠的保護(hù)作用[J];長江大學(xué)學(xué)報(自科版);2014年18期

6 Fernando Magro;Jo?o-Bruno Soares;Dália Fernandes;;Venous thrombosis and prothrombotic factors in inflammatory bowel disease[J];World Journal of Gastroenterology;2014年17期

7 張學(xué)蘭;;潰瘍性結(jié)腸炎患者血清促炎因子與抗炎因子的表達(dá)及其關(guān)系[J];山東醫(yī)藥;2014年05期

8 劉蕊潔;葉永安;;潰瘍性結(jié)腸炎血栓前狀態(tài)的中西醫(yī)研究現(xiàn)狀[J];中國中西醫(yī)結(jié)合消化雜志;2013年05期

9 趙海靜;;王長洪教授用活血化瘀法治療潰瘍性結(jié)腸炎的經(jīng)驗探討[J];求醫(yī)問藥(下半月);2013年03期

10 王興木;;血漿AT-Ⅲ、D-D和CRP檢測在潰瘍性結(jié)腸炎中的臨床價值分析[J];放射免疫學(xué)雜志;2013年01期

相關(guān)博士學(xué)位論文 前1條

1 李云海;加味白頭翁湯對潰瘍性結(jié)腸炎大鼠結(jié)腸粘膜損傷修復(fù)作用的機(jī)理研究[D];湖北中醫(yī)藥大學(xué);2012年

相關(guān)碩士學(xué)位論文 前4條

1 徐霞;三皮湯對實驗性小鼠潰瘍性結(jié)腸炎的作用及IL-4、IL-6表達(dá)的變化[D];中南大學(xué);2014年

2 邱家權(quán);痛瀉二草方對肝郁脾虛型潰瘍性結(jié)腸炎模型大鼠NF-κB通路相關(guān)分子表達(dá)影響的研究[D];甘肅中醫(yī)學(xué)院;2014年

3 雒福東;血竭治療活動期潰瘍性結(jié)腸炎的實驗及臨床研究[D];成都中醫(yī)藥大學(xué);2011年

4 閆成秋;潰瘍性結(jié)腸炎瘀血狀態(tài)（血小板活化）的實驗研究[D];長春中醫(yī)藥大學(xué);2009年

，

本文編號：2170244