不同蓋髓材料對(duì)大鼠磨牙牙髓組織病理學(xué)改變的實(shí)驗(yàn)觀察
發(fā)布時(shí)間：2018-07-26 07:56
【摘要】：目的：通過(guò)比較Urbical LC光固化氫氧化鈣、3M Adper Easy One自酸蝕粘結(jié)劑、Gluma脫敏劑及3M Vitrebond光固化玻璃離子在大鼠深齲模型中對(duì)牙髓組織的病理學(xué)變化，為臨床深齲的樹(shù)脂窩洞充填中如何選擇蓋髓材料起參考作用。 方法：選擇河北醫(yī)科大學(xué)實(shí)驗(yàn)中心提供Wistar雄性大鼠72只，體重250~300g,鼠齡5~6個(gè)月,口腔衛(wèi)生狀況良好,無(wú)明顯齲壞、隱裂等牙體硬組織疾病。 根據(jù)不同蓋髓方式隨機(jī)分為A、B、C、D四組，每組18只。A組為對(duì)照組，B、C、D組為實(shí)驗(yàn)組 A組：Urbical LC光固化氫氧化鈣 B組：3M Adper Easy One自酸蝕粘結(jié)劑 C組：Gluma脫敏劑 D組：3M Vitrebond光固化玻璃離子 A組：將大鼠100g/L水合氯醛(3mL/kg)腹腔注射麻醉后，仰臥固定，用30ml/L的飽和H2O2棉球清潔口腔，750mg/l乙醇隨機(jī)消毒一側(cè)磨牙區(qū)，選取該側(cè)上頜第一磨牙為實(shí)驗(yàn)牙，用小球鉆在實(shí)驗(yàn)牙合面預(yù)備近髓窩洞，以窩洞底部透紅為標(biāo)準(zhǔn)。隔濕，干燥后，涂一薄層Urbical LC光固化氫氧化鈣，光照20s，涂布3MAdper Easy One自酸蝕粘接劑20s，輕吹5s，光照20s，流動(dòng)樹(shù)脂充填。B組直接涂布粘接劑，流動(dòng)樹(shù)脂充填。C組涂布粘接劑前使用Gluma脫敏劑，D組涂布粘接劑前玻璃離子蓋髓。B、C、D其余步驟同A。 分別于1d、7d、14d，，將大鼠于全麻下用4%多聚甲醛心臟灌流固定1h后，取實(shí)驗(yàn)牙,距根尖1mm處截?cái)嘌栏�，暴露牙�?4%多聚甲醛固定48h,10%EDTA脫鈣4～6周,梯度乙醇脫水、石蠟包埋,將標(biāo)本按平行牙長(zhǎng)軸方向做頰舌向連續(xù)切片，制備5μm厚切片，HE染色和Gram細(xì)菌染色。 采用Costa和Hebling的評(píng)分標(biāo)準(zhǔn)，評(píng)估包括炎癥細(xì)胞反應(yīng)、組織破壞程度、反應(yīng)性牙本質(zhì)形成和細(xì)菌染色四種組織學(xué)特征并計(jì)分，使用電子測(cè)量尺測(cè)量各組剩余牙本質(zhì)厚度（RDT）。 結(jié)果： 1、術(shù)后1d，炎癥反應(yīng)中，各組之間差異具有統(tǒng)計(jì)學(xué)意義，P＜0.05，C組炎癥反應(yīng)明顯較輕，與A、B、D三組比較有統(tǒng)計(jì)學(xué)意義，A、B、D三組之間差距無(wú)統(tǒng)計(jì)學(xué)意義。各組在組織紊亂、修復(fù)性牙本質(zhì)形成及細(xì)菌滲透中差異無(wú)統(tǒng)計(jì)學(xué)意義。 2、術(shù)后7d，各組炎性反應(yīng)明顯減輕，各組間炎性反應(yīng)、組織紊亂及修復(fù)性牙本質(zhì)及細(xì)菌滲透差異無(wú)統(tǒng)計(jì)學(xué)意義。 3、術(shù)后14d，各組牙髓組織基本恢復(fù)正常，各組間炎性反應(yīng)、組織紊亂、修復(fù)性牙本質(zhì)形成及細(xì)菌滲透差異無(wú)統(tǒng)計(jì)學(xué)意義。 4、由于觀察時(shí)間較短，各組未見(jiàn)明顯修復(fù)性牙本質(zhì)形成及細(xì)菌滲透。 5、炎性反應(yīng)程度及組織紊亂程度與剩余牙本質(zhì)厚度呈負(fù)相關(guān)。 結(jié)論： 1、Gluma脫敏劑可作為一種較好的蓋髓材料，對(duì)牙髓組織刺激性較小。 2、Urbical LC光固化氫氧化鈣組、3M Adper Easy One自酸蝕粘結(jié)劑、3M Vitrebond光固化玻璃離子之間對(duì)牙髓刺激無(wú)統(tǒng)計(jì)學(xué)差異，可認(rèn)為其在對(duì)牙髓刺激方面相似。 3、隨著時(shí)間的延長(zhǎng)，各組炎癥反應(yīng)減輕，最后均能恢復(fù)正常。 4、牙髓炎癥反應(yīng)及組織紊亂程度與剩余牙本質(zhì)厚度呈負(fù)相關(guān)。
[Abstract]:Objective: to compare the pathological changes of Urbical LC photocurable calcium hydroxide 3M Adper Easy One Adper Easy One desensitizer and 3M Vitrebond photocurable glass ion on pulp tissue in rats with deep caries. It plays a reference role in the selection of pulp capping material in resin cavity filling of deep caries. Methods: 72 male Wistar rats, weighing 250 ~ 300g, were selected from the experimental center of Hebei Medical University. The rats were 5 ~ 6 months old. The oral hygiene condition was good and there were no dental hard tissue diseases such as dental caries and cracked teeth. According to different ways of medullary capping, they were randomly divided into four groups. Each group of 18 rats. Group A is the control group. Group D is the experimental group A: Urbical LC light-cured calcium hydroxide group B: 3M Adper Easy One self-etching binder C group C group: Gluma desensitizer group D Group A: 100g/L hydrate (3mL/kg) was anesthetized by intraperitoneal injection. Supine fixation, 30ml/L saturated H2O2 cotton ball was used to clean oral cavity 750mg / l ethanol randomly. The maxillary first molars were selected as experimental teeth. The cavity near pulp fossa was prepared with small ball drill on the experimental occlusal surface, and the red penetration at the bottom of the cavity was taken as the standard. After wetting and drying, apply a thin layer of Urbical LC photocurable calcium hydroxide, illumination 20s, 3MAdper Easy One self-etching adhesive 20s, light blow 5s, illumination 20s, flow resin filling. B group direct coating adhesive. Before using Gluma desensitizer and group D, the other steps of vitreous ion pulp capping were the same as A. The rats were perfused with 4% paraformaldehyde for 1 hour under general anesthesia for 14 days. The experimental teeth were cut off from the root tip 1mm, and the pulp was fixed with 4% polyformaldehyde for 48 hours. The rats were decalcified for 46-week, gradient ethanol was dehydrated, paraffin was embedded. The buccal and tongue sections were made in parallel to the long axis of teeth, and 5 渭 m thick sections were prepared by HE staining and Gram bacterial staining. Costa and Hebling were used to evaluate the four histological features, including inflammatory cell reaction, tissue damage, reactive dentin formation and bacterial staining. (RDT). Was measured using an electronic ruler to measure the thickness of the remaining dentin in each group. Results: 1. In the inflammatory reaction at 1 day after operation, there was a significant difference between the three groups (P < 0.05). The inflammatory response in group C was significantly less than that in group A (P < 0.05), but there was no significant difference between group D and group A (P < 0.05). There was no significant difference in tissue disorder, formation of repaired dentin and bacterial permeation in each group. 2. On the 7th day after operation, the inflammatory reaction of each group was obviously alleviated, and the inflammatory reaction between groups was obviously alleviated. There was no significant difference in tissue disorder, repair dentin and bacterial osmosis. (3) on the 14th day after operation, the pulp tissue of each group returned to normal, inflammatory reaction and disordered tissue were observed in each group. There was no significant difference in the formation of prosthetic dentin and bacterial osmosis. 4. There was no obvious repair dentin formation and bacterial osmosis. 5. The degree of inflammatory reaction and the degree of tissue disorder were negatively correlated with the thickness of residual dentin. Conclusion: 1 Gluma desensitizer can be used as a better pulp capping material. 2Urbical LC photocurable calcium hydroxide group, 3M Adper Easy One self-etching binder and 3M Vitrebond photocurable glass ion had no significant difference on pulp stimulation. It can be considered that the pulp stimulation is similar. 3. With the prolongation of time, the inflammatory reaction of each group is reduced, and finally can return to normal. 4. The degree of inflammatory reaction and tissue disorder of dental pulp is negatively correlated with the thickness of residual dentin.
【學(xué)位授予單位】：河北醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類(lèi)號(hào)】：R783.1
本文編號(hào)：2145366
[Abstract]:Objective: to compare the pathological changes of Urbical LC photocurable calcium hydroxide 3M Adper Easy One Adper Easy One desensitizer and 3M Vitrebond photocurable glass ion on pulp tissue in rats with deep caries. It plays a reference role in the selection of pulp capping material in resin cavity filling of deep caries. Methods: 72 male Wistar rats, weighing 250 ~ 300g, were selected from the experimental center of Hebei Medical University. The rats were 5 ~ 6 months old. The oral hygiene condition was good and there were no dental hard tissue diseases such as dental caries and cracked teeth. According to different ways of medullary capping, they were randomly divided into four groups. Each group of 18 rats. Group A is the control group. Group D is the experimental group A: Urbical LC light-cured calcium hydroxide group B: 3M Adper Easy One self-etching binder C group C group: Gluma desensitizer group D Group A: 100g/L hydrate (3mL/kg) was anesthetized by intraperitoneal injection. Supine fixation, 30ml/L saturated H2O2 cotton ball was used to clean oral cavity 750mg / l ethanol randomly. The maxillary first molars were selected as experimental teeth. The cavity near pulp fossa was prepared with small ball drill on the experimental occlusal surface, and the red penetration at the bottom of the cavity was taken as the standard. After wetting and drying, apply a thin layer of Urbical LC photocurable calcium hydroxide, illumination 20s, 3MAdper Easy One self-etching adhesive 20s, light blow 5s, illumination 20s, flow resin filling. B group direct coating adhesive. Before using Gluma desensitizer and group D, the other steps of vitreous ion pulp capping were the same as A. The rats were perfused with 4% paraformaldehyde for 1 hour under general anesthesia for 14 days. The experimental teeth were cut off from the root tip 1mm, and the pulp was fixed with 4% polyformaldehyde for 48 hours. The rats were decalcified for 46-week, gradient ethanol was dehydrated, paraffin was embedded. The buccal and tongue sections were made in parallel to the long axis of teeth, and 5 渭 m thick sections were prepared by HE staining and Gram bacterial staining. Costa and Hebling were used to evaluate the four histological features, including inflammatory cell reaction, tissue damage, reactive dentin formation and bacterial staining. (RDT). Was measured using an electronic ruler to measure the thickness of the remaining dentin in each group. Results: 1. In the inflammatory reaction at 1 day after operation, there was a significant difference between the three groups (P < 0.05). The inflammatory response in group C was significantly less than that in group A (P < 0.05), but there was no significant difference between group D and group A (P < 0.05). There was no significant difference in tissue disorder, formation of repaired dentin and bacterial permeation in each group. 2. On the 7th day after operation, the inflammatory reaction of each group was obviously alleviated, and the inflammatory reaction between groups was obviously alleviated. There was no significant difference in tissue disorder, repair dentin and bacterial osmosis. (3) on the 14th day after operation, the pulp tissue of each group returned to normal, inflammatory reaction and disordered tissue were observed in each group. There was no significant difference in the formation of prosthetic dentin and bacterial osmosis. 4. There was no obvious repair dentin formation and bacterial osmosis. 5. The degree of inflammatory reaction and the degree of tissue disorder were negatively correlated with the thickness of residual dentin. Conclusion: 1 Gluma desensitizer can be used as a better pulp capping material. 2Urbical LC photocurable calcium hydroxide group, 3M Adper Easy One self-etching binder and 3M Vitrebond photocurable glass ion had no significant difference on pulp stimulation. It can be considered that the pulp stimulation is similar. 3. With the prolongation of time, the inflammatory reaction of each group is reduced, and finally can return to normal. 4. The degree of inflammatory reaction and tissue disorder of dental pulp is negatively correlated with the thickness of residual dentin.
【學(xué)位授予單位】：河北醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類(lèi)號(hào)】：R783.1
【引證文獻(xiàn)】
相關(guān)期刊論文 前1條
1 趙千寧;王健平;佟瑋瑋;蘇丹;余彥衡;;SonicFill超聲樹(shù)脂對(duì)大鼠成牙本質(zhì)細(xì)胞和牙髓微血管的影響[J];中國(guó)組織工程研究;2016年16期
本文編號(hào)：2145366
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