【摘要】：目的通過(guò)觀察麝香烏龍丸對(duì)大鼠佐劑性關(guān)節(jié)炎（Adjuvant Arthritis, AA）模型垂體組織形態(tài)及促腎上腺皮質(zhì)激素（adrenocorticotropic hormone, ACTH）、β-內(nèi)啡肽（β-endorphin, β-EP）表達(dá)的影響，探討麝香烏龍丸治療類風(fēng)濕關(guān)節(jié)炎（Rheumatoid arthritis, RA）的鎮(zhèn)痛作用機(jī)制，為其臨床應(yīng)用提供實(shí)驗(yàn)與理論依據(jù)。 方法將60只雄性Wistar大鼠適應(yīng)性喂養(yǎng)7d后，隨機(jī)抽取12只，作為正常組。除正常組外，其余48只大鼠均使用乙醚麻醉，于右后足跖注射完全弗氏佐劑（Freund’s complete adjuvant, FCA），建立AA大鼠模型，造模第15天按關(guān)節(jié)炎指數(shù)（arthritis index, AI）隨機(jī)分成模型組、麝香烏龍丸低劑量組、麝香烏龍丸中劑量組、麝香烏龍丸高劑量組，每組12只。分別給予麝香烏龍丸低、中、高劑量組麝香烏龍丸0.3g·kg-1，0.6g·kg-1，1.2g·kg-1（生理鹽水混懸，分別相當(dāng)于成人用藥劑量的3，6，12倍），正常組與模型組給予灌胃等容量生理鹽水，每日1次，連續(xù)給藥2周。第15天將所有大鼠處死后打開腦腔，取出垂體，垂體組織形態(tài)觀察采用蘇木素—伊紅（HE）染色方法，在光鏡下觀察各組垂體組織形態(tài)的變化，，ACTH、β-EP的表達(dá)變化采用免疫組化法進(jìn)行觀察，統(tǒng)計(jì)陽(yáng)性細(xì)胞表達(dá)率。 結(jié)果1對(duì)大鼠一般情況的影響：正常組大鼠飲食正常，皮毛光亮，精神狀態(tài)佳。其余大鼠注射FCA后出現(xiàn)注射足腫脹并出現(xiàn)左后足及前足紅腫，活動(dòng)減少，飲食飲水減少，毛發(fā)稀疏、無(wú)光澤，行走困難。給藥期間，麝香烏龍丸治療組大鼠較模型組一般情況較好，反應(yīng)靈敏，活動(dòng)增多，足踝和足掌腫脹逐漸減輕，皮毛有光澤。2對(duì)大鼠AI的影響：與正常組相比造模各組大鼠AI顯著升高（P＜0.05）。治療后與模型組比較，麝香烏龍丸低、中、高各組AI均明顯下降（P＜0.05），尤以麝香烏龍丸高劑量組下降明顯。3對(duì)大鼠垂體組織形態(tài)的影響：光鏡下觀察各組HE染色后切片發(fā)現(xiàn)，與正常組相比，其余各組垂體組織形態(tài)均無(wú)明顯改變。4對(duì)垂體ACTH表達(dá)的影響：采用免疫組化實(shí)驗(yàn)方法觀察各組大鼠垂體組織內(nèi)ACTH表達(dá)變化，結(jié)果各組ACTH細(xì)胞陽(yáng)性率分別為正常組40.23±3.43，模型組56.82±3.97，麝香烏龍丸低劑量組53.14±4.16，麝香烏龍丸中劑量組50.14±3.39，麝香烏龍丸高劑量組46.87±3.31。與正常組相比，其余4組ACTH細(xì)胞陽(yáng)性表達(dá)均明顯升高，且差異具有統(tǒng)計(jì)學(xué)意義（P＜0.01）。麝香烏龍丸低劑量組與模型組ACTH陽(yáng)性表達(dá)無(wú)顯著性差異（P＞0.05）。麝香烏龍丸中劑量組與麝香烏龍丸高劑量組ACTH細(xì)胞陽(yáng)性表達(dá)明顯低于模型組，差異具有統(tǒng)計(jì)學(xué)意義（P＜0.05或P＜0.01），麝香烏龍丸低、中、高三組對(duì)垂體ACTH陽(yáng)性細(xì)胞表達(dá)的影響有劑量相關(guān)性，其中以麝香烏龍丸高劑量組下調(diào)ACTH表達(dá)最為明顯。5對(duì)垂體β-EP表達(dá)的影響：采用免疫組化實(shí)驗(yàn)方法觀察各組大鼠垂體組織內(nèi)β-EP表達(dá)變化，發(fā)現(xiàn)各組β-EP細(xì)胞陽(yáng)性率分別為正常組30.84±2.19，模型組39.72±2.66，麝香烏龍丸低劑量組43.55±3.59，麝香烏龍丸中劑量組46.69±3.62，麝香烏龍丸高劑量組49.47±3.17。相較于正常組，模型組與麝香烏龍丸低、中、高各組β-EP細(xì)胞陽(yáng)性表達(dá)均明顯升高，且差異具有統(tǒng)計(jì)學(xué)意義（P＜0.01）。麝香烏龍丸低、中、高劑量組β-EP陽(yáng)性表達(dá)均明顯高于模型組，且差異具有統(tǒng)計(jì)學(xué)意義（P＜0.01），麝香烏龍丸低、中、高三組對(duì)垂體β-EP陽(yáng)性細(xì)胞表達(dá)的影響有劑量相關(guān)性，其中以麝香烏龍丸高劑量組升高β-EP表達(dá)最為明顯。結(jié)論麝香烏龍丸可緩解AA大鼠疼痛，其作用機(jī)制與調(diào)節(jié)AA大鼠垂體ACTH、β-EP的表達(dá)有關(guān)。
[Abstract]:Objective to investigate the effect of musk Oolong pills on the morphology of pituitary tissue and the expression of adrenocorticotropic hormone (ACTH) and beta endorphin (beta -endorphin, beta -EP) in the model of Adjuvant Arthritis (AA) model in rats, and to explore the town of musk Oolong pills in the treatment of rheumatoid arthritis (Rheumatoid arthritis, RA). The mechanism of pain action provides experimental and theoretical basis for its clinical application.
Methods after feeding 60 male Wistar rats to 7d, 12 rats were randomly selected as the normal group. Except for the normal group, the other 48 rats were anesthetized with ether, and the complete Freund adjuvant (Freund 's complete adjuvant, FCA) was injected into the right posterior foot. The model of AA rats was established, and the model was randomly selected for fifteenth days according to the arthritis index (arthritis index, AI). Divided into model group, musk Oolong pill low dose group, musk Oolong pill medium dose group, musk Oolong pill high dose group, 12 in each group, respectively give musk Oolong pill low, middle, high dose group of musk Oolong pill 0.3g. Kg-1,0.6g. Kg-1,1.2g. Kg-1 (physiological saline suspension is equivalent to 3,6,12 times of adult drug dosage, respectively), normal group and model group The normal saline was given 1 times a day for 2 weeks. After fifteenth days, all rats were killed and the pituitary was opened. The pituitary tissue morphology was observed by hematoxylin Yi Hong (HE) staining, and the changes of pituitary tissue morphology were observed under the light microscope. The changes of ACTH and beta -EP expression were observed by immunohistochemical method. Positive cell expression rate.
Results the effect of 1 on the general condition of rats: normal diet, bright fur and good mental state. After injection of FCA, the other rats were injected with foot swelling and left hind feet and front foot red and swollen, activities decreased, drinking water decreased, hair sparsely, luster, and difficult walking. The rats in the treatment group of musk Oolong pill were compared with model group during the administration. The general situation was better, the reaction was sensitive, the activity increased, the swelling of the ankle and the palmar palmar gradually lightened, and the lustrous.2 had a significant effect on the AI in rats: the AI of the rats in the model group was significantly higher than that in the normal group (P < 0.05). After the treatment, the musk Oolong pills were lower in the middle and higher groups (P < 0.05), especially in the high dose of musk Oolong pills. The effect of.3 on the morphology of pituitary tissue in rats was observed. Under the light microscope, HE staining was observed in each group. Compared with the normal group, the other groups of pituitary tissue did not significantly change the effect of.4 on the ACTH expression of pituitary gland: immunohistochemical method was used to observe the changes of ACTH expression in the pituitary tissue of each group, and the results of each group were ACTH. The positive rate of the cells in the normal group was 40.23 + 3.43, the model group was 56.82 + 3.97, the low dose group of musk Oolong pill was 53.14 + 4.16, the middle dose group of musk Oolong pill was 50.14 + 3.39, the high dose group of musk Oolong pill was 46.87 + 3.31., and the positive expression of the other 4 groups of ACTH cells increased obviously, and the difference was statistically significant (P < 0.01). Musk The positive expression of ACTH in the low dose group and the model group had no significant difference (P > 0.05). The positive expression of ACTH cells in the middle dose group of musk Oolong pill and the high dose group of musk Oolong pill was significantly lower than that of the model group. The difference was statistically significant (P < 0.05 or P < 0.01). The expression of the pituitary ACTH positive cells in the middle and high three groups of musk Oolong pills was low. In the high dose group of musk Oolong pill, the expression of ACTH expression was the most obvious effect of.5 on the expression of pituitary beta -EP: the expression of beta -EP in pituitary tissue was observed by immunohistochemistry. The positive rate of beta -EP cells in each group was 30.84 + 2.19 in the normal group, 39.72 in the model group, and the musk Oolong pill in the model group. The low dose group was 43.55 + 3.59, the middle dose group of musk Oolong pill was 46.69 + 3.62, the high dose group of musk Oolong pill was 49.47 + 3.17. compared to the normal group. The positive expression of beta -EP cells in the model group and the musk Oolong pill increased obviously, and the difference was statistically significant (P < 0.01). The positive expression of the musk Oolong pill was low, middle and high dose group. It was significantly higher than the model group, and the difference was statistically significant (P < 0.01). The effect of musk Oolong pill low, middle and high three groups on the expression of pituitary beta -EP positive cells was dose-dependent, and the expression of beta -EP in high dose group of musk Oolong pill was most obvious. Conclusion musk ulong pill can relieve pain in AA rats, its mechanism and regulation of AA are large. The expression of ACTH, beta -EP in the rat pituitary is related.
【學(xué)位授予單位】：河北聯(lián)合大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R285.5

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 廖欣;謝海洲診治痹證的臨床特色[J];中國(guó)醫(yī)藥學(xué)報(bào);1998年02期

2 劉健;楊梅云;范海霞;;新風(fēng)膠囊對(duì)類風(fēng)濕關(guān)節(jié)炎的療效及抑郁情緒、血清皮質(zhì)醇的影響[J];長(zhǎng)春中醫(yī)藥大學(xué)學(xué)報(bào);2007年03期

3 劉青松;朱興春;邢艷;蔣紅;蔡燕;雷佳紅;趙明才;楊明輝;唐中;袁國(guó)華;;丹參誘導(dǎo)類風(fēng)濕關(guān)節(jié)炎患者成纖維樣滑膜細(xì)胞中Fas/FasL的表達(dá)[J];第三軍醫(yī)大學(xué)學(xué)報(bào);2010年22期

4 馬麗,郭萬(wàn)首,陶學(xué)濂,吳東海;三種抗風(fēng)濕藥物對(duì)類風(fēng)濕關(guān)節(jié)炎病人關(guān)節(jié)軟骨細(xì)胞NO合成及iNOS活性抑制作用[J];中華風(fēng)濕病學(xué)雜志;2003年04期

5 黃立芳;;類風(fēng)濕關(guān)節(jié)炎伴有抑郁癥狀的相關(guān)因素研究進(jìn)展[J];廣東醫(yī)學(xué);2010年15期

6 覃仕化;汪悅;;痹痛靈顆粒對(duì)膠原誘導(dǎo)性關(guān)節(jié)炎大鼠血清及滑膜組織勻漿中IL-2的影響[J];西部中醫(yī)藥;2011年11期

7 楊昆蓉;舒然;許東云;張穎;劉晗;;類風(fēng)濕關(guān)節(jié)炎使用糖皮質(zhì)激素治療后的中醫(yī)治療探討[J];風(fēng)濕病與關(guān)節(jié)炎;2013年07期

8 劉密鳳;姜楠;王玉明;劉曉娜;王北;;清熱養(yǎng)陰除濕丸對(duì)類風(fēng)濕關(guān)節(jié)炎的治療作用及其機(jī)制[J];國(guó)際檢驗(yàn)醫(yī)學(xué)雜志;2013年15期

9 史永博;鄭世成;李彥民;;仙龍顆粒對(duì)佐劑性關(guān)節(jié)炎大鼠的治療作用及對(duì)血清白細(xì)胞介素-6含量的影響[J];中醫(yī)學(xué)報(bào);2012年01期

10 熊國(guó)林;黃海瀟;謝玲;余祖胤;王欣茹;劉耀文;邢爽;趙振虎;董波;羅慶良;;類風(fēng)濕性關(guān)節(jié)炎大鼠模型的制備[J];解放軍醫(yī)學(xué)雜志;2007年02期

相關(guān)碩士學(xué)位論文 前1條

1 張秋媛;雷公藤多苷聯(lián)合人參皂苷對(duì)膠原誘導(dǎo)關(guān)節(jié)炎大鼠CD4~+CD25~+Foxp3~+調(diào)節(jié)性T細(xì)胞的影響及骨免疫學(xué)機(jī)制探討[D];河北醫(yī)科大學(xué);2012年

本文編號(hào)：2128628