發(fā)布時(shí)間：2018-07-13 16:06

【摘要】：目的:研究地格達(dá)-4味湯對(duì)"幽門結(jié)扎"誘導(dǎo)的肝損傷的作用。方法:取SD大鼠50只,分成5組,即正常、模型、地格達(dá)-4味湯高、中、低(166.4、83.2、41.6 g·kg-1;生藥量分別為557、278.6、139.3 g·kg-1)劑量組,共給藥15 d,末次給藥后禁食不禁水24 h,除正常以外腹腔注射10%水合氯醛麻醉,開腹幽門結(jié)扎,關(guān)腹后單籠飼養(yǎng)18 h,腹主動(dòng)脈取血,取肝臟,測(cè)定相關(guān)指標(biāo)。結(jié)果:模型組與正常組比較血清AST、ALT量明顯增加(P0.01或P0.05),肝組織GSH-PX明顯降低(P0.01);TBIL、DBIL、ALP明顯增高(P0.01);形態(tài)學(xué)觀察肝細(xì)胞有明顯的脂肪變、水腫、邊膜外肝細(xì)胞大量壞死;肝細(xì)胞膜蛋白MRP3表達(dá)上調(diào)至3.5倍、MRP4表達(dá)下調(diào)至0.05倍、核受體Car下調(diào)至2.8倍、核受體Pxr下調(diào)至0.0019倍。各給藥組與模型組比較地格達(dá)-4味湯中、高劑量組AST、ALT明顯降低,GSH-PX明顯增高(P0.01);對(duì)TBIL、DBIL、ALP中劑量組明顯降低(P0.05);肝組織形態(tài)有一定量的好轉(zhuǎn);中劑量組膜蛋白MRP3下調(diào)至0.3倍、MRP4上調(diào)至2.7倍、核受體Car上調(diào)至3066倍、Pxr上調(diào)至506倍。結(jié)論:模型建立成功,地格達(dá)-4味湯具有明顯的保肝作用,且它的作用機(jī)制為對(duì)核受體Car、Pxr的作用,而調(diào)節(jié)MRP3、MRP4表達(dá)發(fā)生改變。據(jù)結(jié)果分析中劑量組效果最佳。
[Abstract]:Objective: to study the effect of Digda-4 decoction on hepatic injury induced by pyloric ligation. Methods: fifty Sprague-Dawley rats were divided into 5 groups: normal, model, high, middle and low (166.4N 83.2N 41.6 g kg-1) group, the dosage of raw drug was 557278.6139.3 g / kg-1, the dosage of digerda-4 was high, medium and low (166.4N 83.2N 41.6 g / kg -1), and the dosage was 557278.6139.3 g / kg-1. After 15 days of administration, fasting for 24 hours, 10% chloral hydrate was injected intraperitoneally to anesthetize, ligation of abdominal pylorus, feeding for 18 hours after abdominal closure. Blood was taken from abdominal aorta, liver was taken and related indexes were measured. Results: compared with the normal group, the serum alt level in the model group was significantly higher than that in the normal group (P0.01 or P0.05), while GSH-PX in the liver tissue was significantly decreased (P0.01) and the ALP in the liver tissue was significantly increased (P0.01). The expression of MRP3 was up-regulated to 3.5-fold. MRP4 was down-regulated to 0.05-fold. The expression of nuclear receptor car was down-regulated to 2.8-fold. The expression of nuclear receptor PXR was down-regulated to 0.0019 times. Compared with the model group, the ASTH-PX of the high dose group decreased significantly (P0.01), the middle dose group of TBILD DBILILALP decreased significantly (P0.05), the liver tissue morphology improved, the MRP3 decreased to 0.3 times and the MRP4 increased to 2.7 times. The nuclear receptor car was up-regulated to 3066 times and PXR was increased to 506-fold. Conclusion: the model was established successfully, and Digerda-4 decoction has obvious hepatoprotective effect, and its mechanism is the effect on nuclear receptor Carbonucleus Pxr, while the expression of MRP3 + MRP4 changes. According to the results, the medium dose group was the best.
【作者單位】： 內(nèi)蒙古民族大學(xué);
【基金】：國家自然科學(xué)基金項(xiàng)目(81441105,81360673)
【分類號(hào)】：R29

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本文編號(hào)：2119985