發(fā)布時(shí)間：2018-07-04 10:34

  本文選題：全反式維甲酸 + 神經(jīng)干細(xì)胞��； 參考：《山東醫(yī)藥》2014年22期

【摘要】：目的體外應(yīng)用全反式維甲酸(ATRA)進(jìn)行干預(yù)培養(yǎng)的鼠胚神經(jīng)干細(xì)胞(NSCs)聯(lián)合膠質(zhì)細(xì)胞源性神經(jīng)營(yíng)養(yǎng)因子(GDNF)移植至大鼠損傷脊髓,探討其對(duì)脊髓損傷再生修復(fù)的效果。方法體外應(yīng)用ATRA 1.0μmol/L濃度下誘導(dǎo)NSCs,將60只雌性SD大鼠隨機(jī)分為正常組、假手術(shù)組、損傷對(duì)照組、ATRA干預(yù)組、GDNF組、ATRA+GDNF組,每組10只。損傷對(duì)照組、ATRA干預(yù)組、ATRA+GDNF組、GDNF組大鼠行胸段脊髓全橫斷損傷效果。術(shù)后于移植前1 d、移植后1、2、5、8周,評(píng)估大鼠雙后肢運(yùn)動(dòng)功能,測(cè)定運(yùn)動(dòng)誘發(fā)電位(MEP)評(píng)估神經(jīng)傳導(dǎo)功能,采用免疫熒光染色觀察移植后NSCs存活情況,5-溴脫氧尿嘧啶核苷/膠質(zhì)纖維酸性蛋白(BrdU/GFAP)免疫熒光雙標(biāo)染色觀察星形膠質(zhì)細(xì)胞分化情況,5-溴脫氧尿嘧啶核苷/微管相關(guān)蛋白-2(BrdU/MAP-2)免疫熒光雙標(biāo)染色觀察神經(jīng)元分化情況。評(píng)估軸突生長(zhǎng)情況。結(jié)果 8周后ATRA+GDNF組大鼠行為學(xué)評(píng)分高于其他各組(P0.01)。與ATRA干預(yù)組及GDNF組相比,8周后ATRA+GDNF組脊髓內(nèi)GFAP、MAP-2陽(yáng)性細(xì)胞表達(dá)面積增大(P0.05);移植的NSCs在受損脊髓內(nèi)存活并向周圍遷移,后肢運(yùn)動(dòng)功能較對(duì)照組及ATRA干預(yù)組、GDNF組明顯改善。結(jié)論體外ATRA干預(yù)的NSCs聯(lián)合GDNF移植可有效補(bǔ)充缺失的神經(jīng)細(xì)胞,改善脊髓內(nèi)微環(huán)境,對(duì)大鼠后肢功能的改善優(yōu)于單一應(yīng)用細(xì)胞或神經(jīng)營(yíng)養(yǎng)因子移植。
[Abstract]:Objective to investigate the effect of rat embryonic neural stem cells (NSCs) combined with glial cell derived neurotrophic factor (GDNF) on the regeneration and repair of spinal cord injury (sci) after intervention with all-trans retinoic acid (ATRA) in vitro. Methods NSCs were induced by ATRA 1.0 渭 mol / L in vitro. Sixty female SD rats were randomly divided into normal group, sham-operated group and ATRA GDNF group with 10 rats in each group. The rats in ATRA GDNF group received thoracic spinal cord transection. Motor function of the hind limbs and motor evoked potential (MEP) were evaluated 1 day before transplantation and 8 weeks after transplantation, and the nerve conduction function was evaluated by motor evoked potential (MEP). Observation of survival of NSCs after transplantation by immunofluorescence staining using 5-bromodeoxyuridine / glial fibrillary acidic protein (BrdU / GFAP) immunofluorescence double labeling staining to observe the differentiation of astrocytes with 5-bromodeoxyuridine / microtubule phase BrdU / MAP-2 (BrdU / MAP-2) immunofluorescence double labeling staining was used to observe the differentiation of neurons. Evaluate axon growth. Results after 8 weeks, the behavioral score of ATRA GDNF group was higher than that of other groups (P 0.01). Compared with ATRA intervention group and GDNF group, the area of GFAP-MAP-2 positive cells in the spinal cord of ATRA GDNF group increased after 8 weeks (P0.05), the transplanted NSCs survived and migrated around the injured spinal cord, and the motor function of hind limbs was significantly improved compared with the control group and the ATRA intervention group. Conclusion in vitro ATRA combined with GDNF transplantation can effectively supplement the missing nerve cells and improve the microenvironment of spinal cord. The improvement of hind limb function of rats is better than that of single cell or neurotrophic factor transplantation.
【作者單位】： 瀘州醫(yī)學(xué)院研究生院;瀘州醫(yī)學(xué)院附屬醫(yī)院;
【基金】：四川省衛(wèi)生廳科研資助課題(2008-527-080178) 瀘州市重點(diǎn)科技計(jì)劃資助項(xiàng)目(2008-23-5)
【分類號(hào)】：R651.2

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 孫勇,侍堅(jiān),陸佩華;神經(jīng)營(yíng)養(yǎng)因子與神經(jīng)干細(xì)胞[J];生理科學(xué)進(jìn)展;2002年04期

【共引文獻(xiàn)】

相關(guān)期刊論文 前10條

1 徐恒武;肖健;李校X;;抗精神病藥物對(duì)腦內(nèi)生長(zhǎng)因子調(diào)控作用的研究進(jìn)展[J];國(guó)際精神病學(xué)雜志;2008年01期

2 王波;但齊琴;李玉;;體外培養(yǎng)神經(jīng)干細(xì)胞PDGF、EGF和GDNF的表達(dá)[J];昆明醫(yī)學(xué)院學(xué)報(bào);2010年11期

3 李玉;王波;林娜;王廷華;習(xí)楊彥彬;;體外培養(yǎng)神經(jīng)干細(xì)胞NGF、BDNF和NT-3及其受體trkA、trkB和trkC的表達(dá)[J];昆明醫(yī)學(xué)院學(xué)報(bào);2011年02期

4 趙楠;劉俊;林娜;王廷華;;體外培養(yǎng)神經(jīng)干細(xì)胞CNTF、TGF-β1和IGF-1的表達(dá)[J];昆明醫(yī)學(xué)院學(xué)報(bào);2011年03期

5 趙祥,李懷斌,熊克仁;某些影響神經(jīng)干細(xì)胞增殖分化的某些因素[J];四川解剖學(xué)雜志;2005年03期

6 陳建敏;張曉;;腦源性神經(jīng)營(yíng)養(yǎng)因子在早期人胚神經(jīng)管發(fā)育中的定位表達(dá)[J];四川解剖學(xué)雜志;2006年02期

7 孫勇,侍堅(jiān),富賽里,陸佩華,徐曉明;胚胎神經(jīng)干細(xì)胞移植及膠質(zhì)細(xì)胞源性神經(jīng)營(yíng)養(yǎng)因子對(duì)大鼠脊髓損傷的修復(fù)作用[J];生理學(xué)報(bào);2003年03期

8 廖德豐,陳婉蓉;神經(jīng)干細(xì)胞研究進(jìn)展[J];生物學(xué)教學(xué);2004年02期

9 裴少琨;涂艷陽(yáng);;FGF和BDNF對(duì)神經(jīng)干細(xì)胞誘導(dǎo)分化的影響[J];實(shí)用預(yù)防醫(yī)學(xué);2007年05期

10 趙彤;黃欣;丁學(xué)鋒;吳奎武;吳麗穎;朱玲玲;范明;;不同濃度的添加劑N2和B27對(duì)神經(jīng)干細(xì)胞增殖的影響[J];生物技術(shù)通訊;2012年06期

相關(guān)博士學(xué)位論文 前5條

1 陳虎;電針對(duì)缺血腦損傷大鼠神經(jīng)干細(xì)胞增殖遷移的影響[D];復(fù)旦大學(xué);2004年

2 席愷;神經(jīng)干細(xì)胞耳蝸移植的相關(guān)研究[D];鄭州大學(xué);2005年

3 任秀君;針刺對(duì)高血脂合并腦缺血大鼠神經(jīng)干細(xì)胞和神經(jīng)營(yíng)養(yǎng)因子的影響[D];北京中醫(yī)藥大學(xué);2007年

4 胡琳燕;丹參誘導(dǎo)骨髓間充質(zhì)干細(xì)胞神經(jīng)性分化治療新生鼠缺氧缺血性腦損傷[D];重慶醫(yī)科大學(xué);2008年

5 孫f，

本文編號(hào)：2095787