本文選題：D-檸檬烯 + ALD��； 參考：《青島大學(xué)》2014年碩士論文

【摘要】：目的觀察D-檸檬烯(D-limonene)對酒精性肝損傷大鼠抗氧化活性及對肝組織核因子κB(NF-κB)、環(huán)氧合酶2(Cyclooxygenase-2,COX-2)蛋白表達的影響。方法雄性Wistar大鼠共70只,按體重隨機分為7組。A組為空白對照組;B組為酒精模型組,每日給予50%乙醇灌胃,前2周8 m L/(kg?d),后4周遞增為12m L/(kg?d),繼續(xù)灌胃4周;C、D、E組分別為D-檸檬烯低、中、高劑量組,每日分別給予D-檸檬烯100、200、400 mg/kg灌胃;F組為D-檸檬烯單獨高劑量組,每日給予D-檸檬烯400mg/kg灌胃;G組為甘利欣對照組,每日給予甘利欣200mg/kg。C、D、E、G組酒精劑量同模型組,F組蒸餾水劑量同空白對照組。每組10只,實驗進行6周。末次灌胃禁食12 h后,麻醉,取血,分離血清,留取肝組織。HE染色觀察肝臟組織的病理學(xué)改變;檢測大鼠血漿和肝勻漿中超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽過氧化物酶(GSH-Px)水平;免疫組化法檢測肝組織中NF-κB、COX-2表達情況,計算其陽性表達率。結(jié)果HE染色結(jié)果顯示,酒精模型組大鼠肝組織呈大泡性脂肪變性,并有大量炎性細胞的浸潤;D-檸檬烯各劑量組與酒精模型組相比,脂肪變性呈不同程度減輕,炎性細胞減少。與空白對照組相比,酒精模型組血漿SOD、血漿和肝勻漿GSH-Px活力均降低,血漿和肝勻漿MDA含量明顯升高(P0.05);D-檸檬烯中、高劑量組血漿SOD以及各劑量組血漿和肝勻漿GSH-Px活力均高于酒精模型組,血漿和肝勻漿MDA含量則低于酒精模型組(P0.05)。酒精模型組與空白對照組相比,NF-κB、COX-2表達明顯增多,而D-檸檬烯各劑量組NF-κB、COX-2表達均低于酒精模型組,差異有顯著性(P0.05)。與空白對照組相比,F組大鼠各指標(biāo)無明顯差異(P0.05)。結(jié)論D-檸檬烯能夠增強酒精性肝損傷大鼠體內(nèi)抗氧化酶的活性,減少脂質(zhì)過氧化物的產(chǎn)生,減少肝組織NF-κB、COX-2蛋白表達,從而改善因酒精暴露引起的肝損傷。
[Abstract]:Aim to observe the effects of D-limonene on the antioxidant activity and the expression of nuclear factor 魏 B (NF- 魏 B) -NF- 魏 B (cyclooxygenase-2cyclooxygenase-2COX-2) protein in rats with alcoholic liver injury. Methods A total of 70 male Wistar rats were randomly divided into 7 groups according to their body weight. Group A was a blank control group and group B was treated with alcohol model group. 50% ethanol was administered daily by gavage. In the first 2 weeks, 8 mL 路L / L / kg ~ (-1) d ~ (-1) was added to 12 mL 路L ~ (-1) 路kg ~ (-1) d ~ (-1) at the last 4 weeks. After 4 weeks of continuous intragastric administration, the low, middle and high doses of D- limonene were given to the group C ~ (2 +) and D ~ (100200400) mg/kg daily respectively. The group F was treated with high dose of Dlimonene alone, respectively, after 4 weeks of oral administration of D-limonene. Group G was treated with D-limonene 400mg/kg intragastrically as control group, and the alcohol dosage of group C was 200 mg / kg / d. The dose of distilled water in group F was the same as that in group C (P < 0.05), and the dose of distilled water in group F was the same as that in group C (P < 0.05). The experiment was conducted for 6 weeks with 10 rats in each group. After the last gastric fasting for 12 hours, the liver was anesthetized, blood samples were taken, serum was isolated, liver tissue was stained with HE to observe the pathological changes of liver tissue. The levels of superoxide dismutase (SOD), malondialdehyde (MDAA) and glutathione peroxidase (GSH-Px) in plasma and liver homogenate of rats were detected, and the expression of COX-2 in liver tissue was detected by immunohistochemical method, and the positive rate of COX-2 was calculated. Results HE staining showed that the liver tissue of the alcohol model group showed vesicular steatosis, and there were a large number of inflammatory cells infiltrated in D- limonene groups. Compared with the alcohol model group, the steatosis was reduced in varying degrees and the inflammatory cells were decreased in each dose group. Compared with control group, plasma SOD, GSH-Px activity in plasma and liver homogenate in alcohol model group decreased, MDA content in plasma and liver homogenate increased significantly in D- limonene. The activity of GSH-Px in plasma and liver homogenate in high dose group was higher than that in alcohol model group, and MDA content in plasma and liver homogenate was lower than that in alcohol model group. Compared with the control group, the expression of COX-2 in the alcohol model group was significantly higher than that in the control group, while the expression of COX-2 in the D- limonene group was lower than that in the alcohol model group (P 0.05). Compared with the blank control group, there was no significant difference in each index between the F group and the blank control group (P 0.05). Conclusion D- limonene can enhance the activity of antioxidant enzymes, reduce the production of lipid peroxides and decrease the expression of NF- 魏 B _ (1) COX-2 protein in the liver tissue of rats with alcoholic liver injury, thereby improving the liver injury induced by alcohol exposure.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R575

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