本文選題：番茄紅素 + 梗阻性黃疸　； 參考：《南華大學(xué)》2014年碩士論文

【摘要】：目的： 探討番茄紅素對(duì)梗阻性黃疸大鼠肝臟的保護(hù)作用，，為番茄紅素在臨床應(yīng)用提供理論依據(jù)。 材料與方法： 1.動(dòng)物分組：健康雄性成年SD大鼠30只（由湖南省人民醫(yī)院臨床研究所提供），體重250±20g。飼養(yǎng)環(huán)境：溫度保持在22℃，濕度保持在50%-60%，干凈衛(wèi)生。操作前后均自由進(jìn)食、自由飲水，12小時(shí)晝夜規(guī)律生活。大鼠隨機(jī)分為3組：SO-MO組：假手術(shù)玉米油口服組；BDL-MO組：梗阻性黃疸玉米油口服組；BDL-LP組：梗阻性黃疸番茄紅素口服組（番茄紅素溶于0.5ml玉米油中）。 2.動(dòng)物模型制備：各組大鼠：10%水合氯醛按0.3ml/kg腹腔注射麻醉，取仰臥位，四肢固定，絡(luò)合碘消毒，鋪孔單，腹正中白線進(jìn)腹，找到膽總管。（1）SO-MO:予以翻動(dòng)第一肝門后關(guān)腹。術(shù)前3天開(kāi)始至術(shù)后14天連續(xù)予口服玉米油0.5ml。（2）BDL-MO組：予以0#絲線結(jié)扎膽總管后關(guān)腹，術(shù)前3天開(kāi)始至術(shù)后14天連續(xù)予口服玉米油0.5ml。（3）BDL-LP組：予以0#絲線結(jié)扎膽總管后關(guān)腹，術(shù)前3天開(kāi)始至術(shù)后14天連續(xù)予口服番茄紅素100mg/Kg/d（溶于0.5ml玉米油中）。所有口服液體均經(jīng)灌胃器給藥。 3.指標(biāo)檢測(cè)：（1）術(shù)后14天心臟穿刺取血標(biāo)本監(jiān)測(cè)各組大鼠生化指標(biāo)（AST、ALT、D-BIL、GGT）。（2）術(shù)后14天取各組大鼠肝臟標(biāo)本監(jiān)測(cè)（MDA、SOD）。（3）術(shù)后14天取各組大鼠肝組織標(biāo)本監(jiān)測(cè)TNF-α。（4）術(shù)后14天取各組大鼠肝組織標(biāo)本監(jiān)測(cè)DNA損傷指標(biāo)。（5）術(shù)后14天取各組大鼠肝臟標(biāo)本進(jìn)行組織學(xué)監(jiān)測(cè)（匯管區(qū)炎癥、局灶性壞死）。 結(jié)果： 1.各組大鼠血生化指標(biāo) （1）與SO-MO比較，BDL-MO組及BDL-LP組大鼠的AST、ALT、GGT、D-BIL明顯增高，(P＜0.05)，差異有統(tǒng)計(jì)學(xué)意義；而B(niǎo)DL-LP組大鼠的ALT、AST、GGT較BDL-MO組明顯降低（P＜0.05）；BDL-MO組與BDL-LP組大鼠的D-BIL無(wú)明顯差異（P＜0.05）。 2.肝組織的（MDA、SOD） (1)肝臟MDA:BDL-MO組MDA明顯高于SO-MO組，P0.05，差異有統(tǒng)計(jì)學(xué)意義；BDL-LP組MDA明顯低于BDL-MO組，P＜0.05，差異有統(tǒng)計(jì)學(xué)意義；SO-MO組與BDL-LP組未見(jiàn)明顯差異（P＞0.05）。 (2)肝臟SOD：BDL-MO組明顯低于SO-MO組，P＜0.05，差異有統(tǒng)計(jì)學(xué)意義。BDL-LP組SOD明顯低于SO-MO組，P＜0.05，差異有統(tǒng)計(jì)學(xué)意義；而B(niǎo)DL-LP組的SOD較BDL-MO組均高，P＜0.05，差異有統(tǒng)計(jì)學(xué)意義。 3.肝組織TNF-α： BDL-MO組TNF-α明顯高于SO-MO組（P0.05）；BDL-LP組TNF-α明顯低于BDL-MO組（P＜0.05）；SO-MO組與BDL-LP組未見(jiàn)明顯差異，P＞0.05,無(wú)統(tǒng)計(jì)學(xué)意義。 4.肝細(xì)胞DNA損傷： BDL-MO組的DNA損傷指標(biāo)（DNA尾長(zhǎng)，尾矩，尾強(qiáng)度）均大于SO-MO組，P＜0.05，有統(tǒng)計(jì)學(xué)意義；BDL-LP組的DNA損傷指標(biāo)均小于BDL-MO組（P＜0.05），差異有統(tǒng)計(jì)學(xué)意義；BDL-LP組與SO-MO組DNA指標(biāo)未見(jiàn)明顯差異（P＞0.05）。 5.肝組織的組織學(xué)： SO-MO組的肝組織可見(jiàn)大部分匯管區(qū)未見(jiàn)炎癥細(xì)胞浸潤(rùn)，小部分可見(jiàn)少量匯管區(qū)炎癥細(xì)胞浸潤(rùn)。未見(jiàn)局灶性壞死。BDL-MO組的肝組織可見(jiàn)大部分匯管區(qū)大量炎性細(xì)胞浸潤(rùn)，小部分匯管區(qū)少量炎性細(xì)胞浸潤(rùn)�？梢�(jiàn)大量局灶性壞死甚至廣泛壞死。BDL-LP組的肝組織絕大多數(shù)可見(jiàn)輕微炎癥表現(xiàn)，小部分未見(jiàn)局灶性壞死，大部分局灶性壞死，較BDL-MO組明顯減輕，P＜0.05，有統(tǒng)計(jì)學(xué)意義。 結(jié)論： 1.使用番茄紅素可能能夠減輕梗阻性黃疸造成的肝細(xì)胞損傷；
[Abstract]:Objective:
Objective to investigate the protective effect of lycopene on the liver of rats with obstructive jaundice, so as to provide a theoretical basis for clinical application of lycopene.
Materials and methods:
1. group of animals: 30 healthy male adult SD rats (provided by Hunan People's Hospital Clinical Research Institute), weight 250 + 20g. feeding environment: temperature kept at 22, humidity kept in 50%-60%, clean and sanitary. Free feeding, free drinking water, 12 hours of day and night regulation of life. Rats were randomly divided into 3 groups: group SO-MO: sham operation jade Oral group of mica oil, group BDL-MO: oral jaundice oral group of obstructive jaundice and group BDL-LP: oral obstructive jaundice lycopene oral group (lycopene dissolves in 0.5ml corn oil).
2. animal model preparation: rats in each group: 10% chloral chloral anaesthesia by 0.3ml/kg intraperitoneal injection, take the supine position, limbs fixed, complex iodine disinfection, paving single, abdominal line into the abdomen, to find the common bile duct. (1) SO-MO: to turn the first hepatic portal after the closure of the abdomen. 3 days before the operation to 14 days after the operation, 0.5ml. (2) BDL-MO group: 0# The silk thread ligated the common bile duct and closed the abdomen after the operation, 3 days before the operation and 14 days after the operation, the oral corn oil 0.5ml. (3) BDL-LP group was given after the operation, and the 0# thread was ligated to the common bile duct and closed the abdomen. The oral lycopene 100mg/Kg/d (dissolved in the 0.5ml corn oil) was given 3 days before the operation, and the oral liquid was given by the gavage apparatus for 14 days after the operation.
3. index test: (1) 14 days after the operation, the blood samples were monitored by cardiac puncture (AST, ALT, D-BIL, GGT). (2) the liver specimens from each group were monitored at 14 days after operation (MDA, SOD). (3) the liver tissue specimens from each group were monitored for TNF- a (4) on the 14 day after the operation (4) on the 14 day after operation to monitor the index of DNA damage in each group of rats. (5) 14 days after operation. The liver specimens of each group were taken for histological monitoring (focal area inflammation and focal necrosis).
Result錛

本文編號(hào)：1964210