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  本文選題：p38絲裂原活化蛋白激酶 + 痛覺過敏�。� 參考：《寧夏醫(yī)科大學》2014年碩士論文

【摘要】：目的蛛網膜下腔注射p38MAPK抑制劑SB203580，觀察其對瑞芬太尼輸注后切開痛痛覺過敏的影響，及脊髓背角p-p38MAPK陽性細胞數(shù)目的變化，探討脊髓背角p38MAPK活化在瑞芬太尼誘發(fā)術后痛覺過敏中的作用。方法取蛛網膜下腔置管成功的雄性SD大鼠40只，體重230-270g，采用數(shù)字表法將其隨機分為5組：對照組、切口痛組、瑞芬太尼組、p38MAPK特異性抑制劑SB203580組（p38MAPK抑制劑組）和SB203580的溶媒二甲基亞砜DMSO組（溶媒二甲基亞砜組）。對照組、切口痛組、瑞芬太尼組大鼠蛛網膜下腔分別給予NS10μL，p38抑制劑組和溶媒二甲亞楓組大鼠蛛網膜下腔分別給予0.1%SB20358010μL和2%DMSO10μL；切口痛組大鼠在1.5%異氟烷吸入麻醉下行右側跖底切口手術，對照組大鼠不行右側跖底手術，其余三組均在瑞芬太尼持續(xù)泵注下行右側跖底切開手術。采用熱輻射刺激儀和von Frey纖維絲分別測定雙側后爪熱刺激縮爪潛伏期（PWL）及機械性痛閾（PWT）。應用免疫組織化學法檢測脊髓背角p-p38MAPK表達的變化。結果與切口痛組比較，瑞芬太尼組、溶媒二甲亞楓組在術后4h、1d、2d、3d雙側跖底PWL、PWT值均降低，雙側脊髓背角p-p38MAPK表達增多；與瑞芬太尼組比較，，p38抑制劑組雙側跖底的PWT值、手術側跖底PWL值增高，雙側脊髓背角p-p38MAPK表達減少。結論脊髓背角p38MAPK活化可能參與了瑞芬太尼誘發(fā)的術后痛覺過敏。
[Abstract]:Objective to observe the effect of subarachnoid injection of p38MAPK inhibitor SB203580 on remifentanil after infusion of remifentanil and the changes of p-p38MAPK positive cells in the dorsal horn of spinal cord. To investigate the role of p38MAPK activation in remifentanil induced postoperative hyperalgesia. Methods Forty male Sprague-Dawley rats (230-270g) with successful subarachnoid catheterization were randomly divided into five groups: control group, incision pain group, and incision pain group. In remifentanil group, p38 MAPK specific inhibitor, SB203580 group, and SB203580 solute dimethyl sulfoxide DMSO group (dimethyl sulfoxide group). Control group, incision pain group, The rats in remifentanil group were given subarachnoid NS10 渭 L and 2%DMSO10 渭 L, respectively, and the rats in incision pain group were treated with right plantar floor incision under 1.5% isoflurane inhalation anesthesia, and the rats in remifentanil group were given 0.1%SB20358010 渭 L and 2%DMSO10 渭 L in subarachnoid space, respectively. Rats in the control group were not operated on the right plantar floor, and the other three groups were treated with remifentanil continuous pump for right plantar floor incision. Thermal radiation stimulator and von Frey filament were used to measure the latent period of paw contraction and mechanical pain threshold. Immunohistochemical method was used to detect the expression of p-p38MAPK in spinal dorsal horn. Results compared with incision pain group, remifentanil group and dimethyl submaple group decreased the PWT value of bilateral plantar floor PWL and increased the expression of p-p38MAPK in bilateral dorsal horn of spinal cord on the 1st and 2nd day after operation, and compared with remifentanil group, the PWT value of bilateral plantar floor in remifentanil group was higher than that in remifentanil group. The PWL of the plantar floor increased and the expression of p-p38MAPK decreased in the bilateral dorsal horn of spinal cord. Conclusion the activation of p38MAPK in spinal dorsal horn may be involved in postoperative hyperalgesia induced by remifentanil.
【學位授予單位】：寧夏醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R614

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