本文選題：鐵過量 + 仔鼠��； 參考：《營養(yǎng)學報》2015年02期

【摘要】：目的研究孕期及哺乳期母鼠鐵過量補充對仔鼠神經(jīng)反射及學習記憶能力的影響。方法將受孕第0日孕鼠隨機分為正常對照組(對照組)、低劑量鐵補充組(低鐵組)和高劑量鐵補充組(高鐵組)三組,每組10只,單籠飼養(yǎng),自由進食全價顆粒(含鐵量為370 mg/kg),飲用去離子水。自妊娠第l日開始對孕鼠進行鐵干預,腹腔注射右旋糖酐鐵,每2日1次,直至仔鼠斷乳。對照組隔天腹腔注射生理鹽水2 ml,低鐵組、高鐵組分別隔天腹腔注射30、120 mg/kg·bw右旋糖酐鐵,注射前用生理鹽水將每只孕鼠所需右旋糖酐鐵注射液稀釋為2 ml。連續(xù)5w每周稱量仔鼠體質(zhì)量,應用比色法測量仔鼠血清鐵含量;應用懸崖回避、平面翻正、負趨地性試驗測定仔鼠的神經(jīng)反射功能;應用Morris水迷宮試驗測定仔鼠學習記憶能力。5w后對仔鼠進行麻醉,斷頭取血,靜置30 min,3 000 r/min離心10 min,取上清,于-20℃凍存。結果高鐵組血清鐵含量為61.87μmol/L,顯著高于對照組的38.34μmol/L和低鐵組的38.45μmol/L(P0.05)。神經(jīng)反射試驗結果顯示,負趨地性試驗中高鐵組仔鼠的達標日齡為8.60 d,顯著高于對照組的6.40 d和低鐵組的5.40 d(P均0.05),低鐵組與對照組相比差異無統(tǒng)計學意義(P0.05);懸崖回避試驗中,高鐵組仔鼠的達標為日齡6.90 d,顯著高于對照組的3.60 d和低鐵組的3.30 d(P均0.05),低鐵組與對照組相比差異無統(tǒng)計學意義(P0.05);平面翻正試驗中,高鐵組仔鼠達標日齡為6.10 d,顯著高于對照組的3.90 d和低鐵組的2.70 d(P均0.05),且低鐵組顯著低于對照組(P0.05)。Morris水迷宮實驗中,訓練的第2日,高鐵組仔鼠平均逃避潛伏期為37.80 s,顯著長于對照組的26.85 s(P0.05),但與低鐵組的31.93 s相比差異無統(tǒng)計學意義(P0.05)。訓練的第5日,高鐵組仔鼠平均逃避潛伏期為23.76 s,顯著長于對照組的19.73 s和低鐵組的15.77 s(P均0.05),兩日中低鐵組與對照組相比差異均無統(tǒng)計學意義(P均0.05)。結論孕期及哺乳期母鼠過量補充可損傷仔鼠神經(jīng)反射功能,并對仔鼠的學習記憶能力可能產(chǎn)生不良影響。
[Abstract]:Objective to study the effect of iron excess supplementation on the neural reflex and learning and memory ability of offspring rats during pregnancy and lactation. Methods zeroth pregnant rats were randomly divided into normal control group (control group), three groups of low dose iron supplementation group (low iron group) and high dose iron supplement group (Gao Tiezu), 10 rats in each group, and free feeding of full price granules. The iron content was 370 mg/kg), drinking deionized water. From the first day of pregnancy, the pregnant rats were treated with iron intervention and intraperitoneal injection of iron dextran, 1 times every 2 days until the offspring were weaned. The control group was intraperitoneally injected with saline 2 ml, low iron group, and the high iron group was intraperitoneally injected with 30120 mg/ kg. BW dextran iron in the abdominal cavity every other day. The Iron Dextran Injection of pregnant rats was diluted to 2 ml. to weigh the body mass per week of 5W mice. The serum iron content was measured by colorimetric method. The nerve reflex function of the offspring was measured by the cliff avoidance, plane reversal and negative chemotactic test, and the Morris water maze test was used to determine the learning and memory ability of the offspring to be anaesthetized by the offspring of the offspring. Blood, 30 min, 3000 r/min centrifugation 10 min, Torikami Kiyo, and cryopreserved at -20 C. The serum iron content of the high iron group was 61.87 mu mol/L, which was significantly higher than that of the control group of 38.34 micron mol/L and the low iron group. The results of neural reflex test showed that the age of the mice in the negative chemotactic test was 8.60 D, significantly higher than that of the high iron group. The 6.40 D in the control group and 5.40 D in the low iron group (P 0.05), there was no significant difference between the low iron group and the control group (P0.05). In the cliff avoidance test, the standard of the high iron group was 6.90 D, significantly higher than the 3.60 D in the control group and 3.30 D in the low iron group (P all 0.05), and the low iron group had no statistical difference (P0.05) compared with the control group (P0.05). In the positive test, the age of the mice in the high iron group was 6.10 D, which was significantly higher than the 3.90 D in the control group and 2.70 D in the low iron group (P 0.05), and the low iron group was significantly lower than the control group (P0.05).Morris water maze experiment. The average escape latency of the high speed iron group was 37.80 s, which was significantly longer than the control group of 26.85 s (P0.05), but it was compared with the low iron group. The difference was not statistically significant (P0.05) compared with the 31.93 s. The average escape latency of the high speed iron group was 23.76 s, which was significantly longer than 19.73 s in the control group and 15.77 s in the low iron group (P 0.05). There was no significant difference between the middle and low iron groups (P 0.05) on two days (P 0.05). Conclusion excessive supplementation in pregnant and lactation rats could be impaired. The nerve reflex function of injured offspring rats may have adverse effects on learning and memory ability of offspring rats.
【作者單位】： 青島大學醫(yī)學院公共衛(wèi)生學院;
【基金】：國家自然科學基金(No.81373000)
【分類號】：R153.1

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本文編號：1904294