本文選題：急性軟組織損傷 + 活血化瘀利水方��； 參考：《湖南中醫(yī)藥大學(xué)》2014年碩士論文

【摘要】：目的：觀察活血化瘀利水方對(duì)大鼠急性軟組織損傷模型損傷癥候指數(shù)、組織形態(tài)學(xué)的影響以及對(duì)損傷局部組織中IL-1p、PGE-2含量的影響,探討活血化瘀利水方對(duì)急性軟組織損傷的治療作用和機(jī)制,以及該中藥復(fù)方對(duì)急性軟組織損傷修復(fù)的作用,為其廣泛應(yīng)用于臨床提供實(shí)驗(yàn)依據(jù)。 方法：將60只SD大鼠隨機(jī)分為空白對(duì)照組、模型對(duì)照組、陽性對(duì)照組(傷科跌打片組)、活血化瘀利水方低劑量組、活血化瘀利水方中劑量組、活血化瘀利水方高劑量組。除空白對(duì)照組外,其余各組均運(yùn)用局部打擊法進(jìn)行造模,造模后第二天開始給藥,于造模后第1、3、5、7天分別觀察各組大鼠局部損傷癥候指數(shù),治療7天后將所有大鼠麻醉,無菌條件下取打擊中心肌肉組織1g,15mg組織進(jìn)行組織勻漿,取上清液檢測(cè)其中IL-1β、PGE-2含量。取0.5cm×0.5cm×0.5cm肌肉組織10%甲醛固定后切片HE染色,進(jìn)行組織形態(tài)學(xué)觀察。 結(jié)果：(1)損傷征候指數(shù)觀察：造模后第1天,各組大鼠損傷癥候指數(shù)評(píng)分無明顯差異(P0.05)；造模后第3天,與模型組比較,活血化瘀利水方低劑量組損傷癥候指數(shù)評(píng)分無明顯差異(P0.05),其余各組均有明顯差異(P0.05或P0.01)；造模后第5、7天,活血化瘀利水方各劑量組和傷科跌打片組均能明顯促進(jìn)損傷局部腫脹消退,皮下瘀斑和皮下出血的消散,與模型對(duì)照組比有明顯的差異(P0.05或P0.01)。 (2)組織形態(tài)學(xué)觀察：與模型對(duì)照組比較,活血化瘀利水方各劑量組、傷科跌打片組損傷局部組織的炎癥明顯減輕,肌纖維的修復(fù)更迅速,病理學(xué)積分明顯降低(P0.05或P0.01),活血化瘀利水方各劑量組與傷科跌打片組相比,病理學(xué)積分無明顯差異(P0.05)。 (3)IL-1β含量檢測(cè)：各治療組損傷局部肌肉組織中IL-1β含量與模型組相比均明顯降低(P0.05或P0.01)；活血化瘀利水方低劑量組IL-1β含量高于傷科跌打片組(P0.05),中、高劑量組含量與傷科跌打片組相比,無明顯差異(P0.05)。 (4)PGE-2含量檢測(cè)：活血化瘀利水方各劑量組均能明顯抑制損傷局部肌肉組織中PGE-2含量的升高,與傷科跌打片組相比,無統(tǒng)計(jì)學(xué)意義(P0.05)；活血化瘀利水方各劑量組和傷科跌打片組與模型組和空白組比較均有統(tǒng)計(jì)學(xué)意義(P0.05或P0.01)。 結(jié)論：活血化瘀利水方具有較明顯的抗炎消腫,并能較好促進(jìn)損傷軟組織修復(fù)的作用。其治療軟組織損傷的療效機(jī)理之一與部分抑制損傷局部組織中炎性細(xì)胞因子IL-1β、PGE-2的產(chǎn)生有關(guān)。
[Abstract]:Objective: To observe the effect of Huoxue Huayu Li Shui Recipe on the injury syndrome index, histomorphology and the content of IL-1p and PGE-2 in the local tissues of rats with acute soft tissue injury, and to explore the therapeutic effect and mechanism of Huoxue Huayu Li Shui Fang on acute soft tissue injury, as well as the repair of acute soft tissue injury by the Chinese medicine compound. It provides experimental evidence for its wide application in clinic.
Methods: 60 SD rats were randomly divided into the blank control group, the model control group, the positive control group (Department of Traumatology Dieu tablet group), the low dose group of Huoxue Huayu Li Shui Fang, the middle dose group of Huoxue Huayu Li Shui Fang, the high dose group of Huoxue Huayu Li Shui Fang, and the other groups except the blank control group. The local injury syndrome index of the rats in each group was observed at the first 1,3,5,7 day after the model, and all the rats were anesthetized for 7 days after the treatment. The 1g of the muscle tissue in the center was taken under aseptic conditions and the tissue homogenate of the 15mg tissue was carried out. The content of IL-1 beta and PGE-2 was detected by the supernatant. 0.5cm x 0.5cm x 0.5cm muscle tissue was fixed to the HE staining after 10% formaldehyde fixation. Color, histomorphology observation.
Results: (1) observation of injury syndrome index: there was no significant difference in the score of injury syndrome index (P0.05) in each group at first days after modeling (P0.05). There was no significant difference between the model group and the model group at third days after the model group, and there was no significant difference (P0.05) in the low dose group (P0.05 or P0.01) in the other groups. Each dose group of Huoxue Huayu Li Shui Fang and the group of Dieu tablet in Department of Traumatology all could obviously promote the local swelling and decline of the injury, and the subcutaneous ecchymosis and subcutaneous hemorrhage were obviously different from the model control group (P0.05 or P0.01).
(2) observation of histomorphology: compared with the model control group, the inflammation of the local tissue in the Department of Traumatology of Huoxue Huayu Li Shui Fang was obviously reduced, the repair of muscle fiber was more rapid and the integral of pathology was obviously reduced (P0.05 or P0.01). Significant difference (P0.05).
(3) IL-1 beta content detection: the content of IL-1 beta in the injured local muscle tissue of the treatment group was significantly lower than that in the model group (P0.05 or P0.01), and the content of IL-1 beta in the low dose group of Huoxue Huayu Li Shui Fang was higher than that of the Department of Traumatology (P0.05), and there was no significant difference between the high dose group and the Department of Traumatology drop group (P0.05).
(4) PGE-2 content detection: each dose group of Huoxue Huayu Li Shui Fang can obviously inhibit the increase of PGE-2 content in the injured local muscle tissue, and there is no statistical significance compared with the Department of Traumatology Diping group (P0.05), and there are statistical significance (P0.05 or P0.01) in each dose group of Huoxue Huayu Li Shui Fang and the group of the Department of Traumatology to the model group and the blank group.
Conclusion: the prescription of Huoxue Huayu Li Shui has obvious anti inflammatory and detumescence, and can promote the repair of soft tissue. One of the therapeutic mechanisms for the treatment of soft tissue injury is related to the inhibition of the production of inflammatory cytokines IL-1 beta and PGE-2 in the local tissue.

【學(xué)位授予單位】：湖南中醫(yī)藥大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R285.5

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