本文選題：骨質(zhì)疏松 + 腎虛�。� 參考：《中華中醫(yī)藥學(xué)刊》2015年12期

【摘要】：目的:探討補(bǔ)腎活血顆粒對去勢大鼠的腎虛癥狀的改善作用及作用機(jī)制,為臨床防治骨質(zhì)疏松癥采用補(bǔ)腎治本的原則提供理論依據(jù)。方法:24只雌性大鼠單純隨機(jī)分為補(bǔ)腎活血組、模型組、假手術(shù)組,每組8只。補(bǔ)腎活血組與模型組麻醉后行雙側(cè)卵巢切除術(shù),假手術(shù)組麻醉后行假手術(shù)處理,飼養(yǎng)3個月確認(rèn)造模成功后各組分別給予補(bǔ)腎活血顆粒、生理鹽水、生理鹽水灌胃,90 d后進(jìn)行3 d大鼠的一般情況觀察及生理狀態(tài)的量化檢測,然后麻醉下處死大鼠,用電子天平稱取垂體、腎、腎上腺、子宮的濕重,采用Elisa法進(jìn)行ACTH、E2、T3、T4的血清學(xué)檢測。結(jié)果:補(bǔ)腎活血組大鼠的體毛光滑略顯稀疏、行動活潑、反應(yīng)尚可、精神尚佳、食欲可、身體肥胖、略有嗜睡、眼睛明亮、眼角干燥、二便基本正常等表現(xiàn);活動度、肛溫及進(jìn)食量均高于模型組,存在顯著性差異(P0.01),與假手術(shù)組無明顯差異(P0.05);血壓與模型組及假手術(shù)組比較無明顯差異(P0.05);血清ACTH、E2、T3、T4的水平明顯高于模型組,具有顯著性差異(P0.01),與假手術(shù)組無明顯差異(P0.05);垂體、腎上腺、腎的濕重均明顯高于模型組,存在顯著性差異(P0.01),與假手術(shù)組比較無統(tǒng)計學(xué)意義(P0.05),但子宮的濕重高于模型組,低于假手術(shù)組,均存在顯著性差異(P0.05)。結(jié)論:補(bǔ)腎活血顆粒能夠明顯的改善去勢大鼠的腎虛癥狀,并且推測調(diào)節(jié)下丘腦-垂體-靶腺軸是補(bǔ)腎活血顆粒改善去勢大鼠腎虛癥狀的作用機(jī)制。
[Abstract]:Objective: to investigate the effect and mechanism of Bushen Huoxue granule on kidney deficiency in ovariectomized rats, and to provide a theoretical basis for clinical prevention and treatment of osteoporosis. Methods Twenty four female rats were randomly divided into three groups: tonifying kidney and activating blood circulation group, model group and sham operation group, 8 rats in each group. The model group and the model group were given bilateral ovariectomy after anesthesia, and sham operation group was given sham-operation treatment after anesthesia. After 3 months of feeding, each group was given Bushen Huoxue granule and physiological saline respectively. After 90 days of intragastric administration of normal saline, the rats were observed in general condition and measured quantitatively in physiological state for 3 days. Then the rats were killed under anesthesia, and the wet weights of pituitary, kidney, adrenal gland and uterus were taken with electronic balance. The serological detection of ACTHN E _ 2 T _ 3 T _ 4 was carried out by Elisa method. Results: in the Bushen and Huoxue group, the body hair was smooth and sparse, active, reactive, good in spirit, appetite, obesity, slightly drowsiness, bright eyes, dry eyes, normal activity, etc. The anal temperature and food intake were higher than those in the model group (P 0.01), but there was no significant difference between the model group and the sham operation group (P 0.05), the blood pressure was not significantly different from the model group and the sham operation group (P 0.05), and the serum level of ACTHG E _ 2T _ 3 T _ 4 was significantly higher than that of the model group. The wet weight of pituitary, adrenal and kidney were significantly higher than that of model group (P 0.01), but the wet weight of uterus was higher than that of model group (P < 0.01), but the wet weight of uterus was higher than that of model group (P < 0.05). There was significant difference between sham operation group and sham operation group (P 0.05). Conclusion: Bushen Huoxue granule can obviously improve the symptoms of kidney deficiency in ovariectomized rats, and it is speculated that regulating hypothalamus-pituitary-target gland axis is the mechanism of Bushen Huoxue granule to ameliorate the symptoms of kidney deficiency in ovariectomized rats.
【作者單位】： 浙江中醫(yī)藥大學(xué)附屬溫州中西醫(yī)結(jié)合醫(yī)院;浙江省骨傷研究所;
【基金】：衛(wèi)生部科學(xué)研究基金項目(WKJ2008-2-032) 浙江省中醫(yī)管理局青年基金項目(2014ZQ025) 溫州市科技局項目(Y2014022)
【分類號】：R285.5

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