本文選題：羅哌卡因 + 驚厥　； 參考：《石河子大學(xué)》2014年碩士論文

【摘要】：目的:研究羅哌卡因所導(dǎo)致的毒性驚厥對幼年SD鼠學(xué)習(xí)記憶能力及海馬區(qū)星型膠質(zhì)纖維酸性蛋白(GFAP)表達(dá)的影響。 方法:選用60只4周齡SD幼鼠按照隨機(jī)數(shù)字表法分為羅哌卡因組(以Racine驚厥分類中V級為標(biāo)準(zhǔn)制作模型)和生理鹽水組(每組30只)。2組各取20只,并根據(jù)不同取材時間點又分為注射后24h、3d、7d、60d4個亞組（每組5只）。選取幼鼠海馬組織，采用免疫組化法行海馬區(qū)星型膠質(zhì)纖維酸性蛋白GFAP的形態(tài)變化并計數(shù);剩余10只于1~3d、5~7d、58~60d行進(jìn)行水迷宮平臺潛伏期測試。 結(jié)果:水迷宮定向航行實驗中羅哌卡因組在毒性驚厥后24h和第3d的逃避潛伏期均長于對照組（P＜0.05)，在第7d和第以后的60d，與對照組已無明顯差別（P＞0.05）。羅哌卡因組的星型膠質(zhì)細(xì)胞GFAP的表達(dá)明顯弱于對照組，表現(xiàn)為胞體較小，突起細(xì)而短，，染色淺淡，且GFAP陽性細(xì)胞在數(shù)量上顯著低于對照組（P＜0.05），尤以麻醉后第三天最為顯著，細(xì)胞數(shù)呈先減少后增加的動態(tài)過程。 結(jié)論:幼鼠單次羅哌卡因毒性驚厥對其學(xué)習(xí)記憶有一過性影響，推測與星型膠質(zhì)纖維酸性蛋白功能有關(guān)。
[Abstract]:Objective: To study the effects of ropivacaine induced convulsion on learning and memory ability and astrocyte glial fibrillary acidic protein (GFAP) expression in hippocampus of young SD rats.
Methods: 60 4 week old SD young rats were divided into ropivacaine group according to random number table method (the standard making of V class in Racine convulsion classification) and.2 group of saline group (30 group of each group) each of 20 groups, and then divided into 24h, 3D, 7d, 60d4 subgroup (5 each group) after the different sampling time points. The hippocampus tissue of young rats was selected and immunized. The morphological changes of astrocyte glial fibrillary acidic protein GFAP in hippocampus were counted by histochemical method, and the remaining 10 in 1~3d, 5~7d and 58~60d were tested by water maze latency test.
Results: the escape latency of 24h and 3D in ropivacaine group was longer than that in the control group (P < 0.05). There was no significant difference between the control group and the control group (P > 0.05) at 7d and later 60d (P > 0.05). The expression of GFAP in ropivacaine group was significantly weaker than that of the control group, which showed that the cell body was smaller and the protuberance was fine. The number of GFAP positive cells was significantly lower than that of the control group (P < 0.05), especially in the third day after anesthesia, and the number of cells showed a dynamic process that decreased first and then increased.
Conclusion: single ropivacaine induced convulsion has a transient effect on learning and memory in young rats. It is speculated that it is related to the function of astroglial fibrillary acidic protein.

【學(xué)位授予單位】：石河子大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R614

【參考文獻(xiàn)】

相關(guān)期刊論文 前3條

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