本文選題：EGCG + 骨質(zhì)疏松　； 參考：《蘇州大學(xué)》2014年碩士論文

【摘要】：目的： 探討表沒食子兒茶素沒食子酸酯（(-)-Epigallocatechin-3-gallate, EGCG）對(duì)雌性去勢（Ovariectomized, OVX）大鼠骨量丟失的早期預(yù)防效果、腹腔注射與灌胃途徑給藥效果和探討其預(yù)防骨量丟失的可能的作用機(jī)制。 方法： 將55只3月齡的正常健康雌性SD大鼠（Sprague-Dawley Rats）分別稱重，并按照各組間體重?zé)o統(tǒng)計(jì)學(xué)差異分為以下5組：A組，共15只：去勢術(shù)后第3天開始以10mg/kg/day的劑量經(jīng)腹腔（intraperitoneal，IP）注射給藥EGCG連續(xù)12周，記為（OVX+early EGCG/IP）、B組，共10只:去勢術(shù)后第7周開始以10mg/kg/day的劑量經(jīng)腹腔注射EGCG連續(xù)給藥6周，記為（OVX+late EGCG/IP）、C組，共10只：去勢術(shù)后第3天開始以10mg/kg/day的劑量經(jīng)灌胃（Oral Feeding， OF）給藥EGCG連續(xù)12周，記為（OVX+early EGCG/OF）、D組，共10只：去勢大鼠無EGCG干預(yù)空白對(duì)照組，記為（OVX），和E組，共10只：假手術(shù)（Sham-operated）正常對(duì)照組，記為（SHAM）。12周后全部予以過量麻醉處死，留取雙側(cè)股骨和脛骨標(biāo)本分別進(jìn)行如下檢測：1、Micro CT掃描，計(jì)算骨密度（BMD）和相關(guān)骨形態(tài)學(xué)參數(shù)，2、進(jìn)行3點(diǎn)彎曲實(shí)驗(yàn)檢測、3、骨組織HE染色，并采用免疫組織化學(xué)染色檢測Sema4D蛋白的表達(dá)情況。 結(jié)果： 骨形態(tài)學(xué)參數(shù)：SHAM組（E組）中大鼠的骨密度（BMD）與松質(zhì)骨骨體積分?jǐn)?shù)（BV/TV）明顯高于其他4組大鼠的BMD以及BV/TV（P0.01），同時(shí)A組大鼠的BMD和BV/TV明顯高于OVX組（D組）大鼠的BMD（P0.05）。SHAM組（E組）中大鼠的松質(zhì)骨平均小梁分離度（Tb.Sp）明顯低于其余4組的Tb.Sp，且A組大鼠的Tb.Sp明顯低于OVX組（D組）大鼠的Tb.Sp（P0.05）。SHAM組（E組）的平均骨小梁厚度（Tb.Th）明顯高于其余各組（P0.01），其余4組間無明顯差異。各組間松質(zhì)骨骨小梁數(shù)量(Tb.N)無明顯差異（P0.05）。3點(diǎn)彎曲應(yīng)力試驗(yàn)結(jié)果：彎曲位移、最大彎曲應(yīng)力在各組之間，差異均無統(tǒng)計(jì)學(xué)意義（P0.05）。骨組織學(xué)觀察：SHAM組（E組）的大鼠骨小梁分布更加致密，，骨小梁數(shù)目更多，而OVX組（D組）的大鼠骨小梁稀疏。A組、B組以及C組的組織學(xué)形態(tài)介于OVX組和SHAM組之間。免疫組化結(jié)果：OVX組（D組）的Sema4D的陽性表達(dá)面積百分比明顯高于其他各組的Sema4D表達(dá)水平（P0.05），SHAM組（E組）與A組之間，Sema4D的陽性表達(dá)面積百分比差異無統(tǒng)計(jì)學(xué)意義（P0.05）。 結(jié)論： 1. EGCG能夠延緩雌性去勢大鼠骨量丟失的進(jìn)程，且在10mg/kg/day的劑量下，較早期的干預(yù)可能優(yōu)于較晚期的干預(yù)，但是EGCG的早期干預(yù)并不能完全阻斷去勢大鼠骨量丟失的進(jìn)程； 2.在10mg/kg/day的劑量下，EGCG預(yù)防雌性去勢大鼠骨量丟失，經(jīng)腹腔注射途徑效果可能優(yōu)于經(jīng)灌胃途徑； 3.去勢大鼠骨組織中的Sema4D表達(dá)高于正常大鼠，且EGCG能一定程度降低去勢大鼠骨組織中Sema4D的表達(dá)。
[Abstract]:Objective: To investigate the early prevention effect of epigallocatechin-3-gallate (EGCGG) on bone mass loss in female ovariectomized (OVXed) rats, the effect of intraperitoneal injection and gavage administration and the possible mechanism of preventing bone loss. Methods: Fifty-five healthy female Sprague-Dawley Ratsrats aged 3 months were weighed and divided into 5 groups: group A according to no significant difference in body weight. A total of 15 rats were given EGCG by intraperitoneal injection of EGCG at the dose of 10mg/kg/day 3 days after ovariectomy for 12 weeks, which was recorded as OVX early EGCG / IP / IP group B (n = 10): from the 7th week after ovariectomy, the dosage of 10mg/kg/day was injected intraperitoneally with EGCG for 6 weeks, which was recorded as OVX late EGCG / IP / IP group C (OVX late EGCG / IP / IP group). Ten rats were treated with oral feeding (10mg/kg/day) for 12 weeks at the dose of 10mg/kg/day on the 3rd day after ovariectomy. The rats were recorded as OVX early EGCG / OFX D group. 10 ovariectomized rats were treated as OVX early EGCG / OFX group without EGCG intervention, as control group, and E group, OVX group, and OVX group (n = 10), and OVX group (n = 10). A total of 10 rats (sham-operated) normal control group, recorded as Sham-operated control group, were all sacrificed under excessive anesthesia after 12 weeks. The specimens of bilateral femur and tibia were taken for the following detection: 1: 1 Micro CT scan, respectively. Bone mineral density (BMD) and related bone morphological parameters were calculated. The expression of Sema4D protein was detected by three point bending test and HE staining in bone tissue. Results: The bone mineral density (BMD) and cancellous bone volume fraction (BV / TVV) of the rats were significantly higher than those of the other four groups (BMD and BV / TVP 0.01), and the BMD and BV/TV of group A were significantly higher than those of BMD(P0.05).SHAM (group D). ) the mean trabecular separation degree of cancellous bone in rats was significantly lower than that in the other four groups, and the Tb.Sp of group A was significantly lower than that of group OVX. (group D) the average trabecular thickness of group Tb.Sp(P0.05).SHAM was significantly higher than that of other groups (P 0.01). There was no significant difference among the four groups. There was no significant difference in the number of trabeculae of cancellous bone in each group. The results showed that the bending displacement and maximum bending stress were not significantly different among the groups. The bone trabecular density and the number of bone trabeculae were more compact in group E than those in group D (OVX group). The histomorphology of group A and group C were between OVX group and SHAM group. Immunohistochemical results showed that the percentage of positive area of Sema4D expression in group D was significantly higher than that in group E (P 0.05). There was no significant difference between group A and group A in the percentage of positive expression of Sema4D (P 0.05). Conclusion: 1. EGCG could delay the process of bone loss in female ovariectomized rats, and at the dose of 10mg/kg/day, the earlier intervention might be better than the late intervention, but the early intervention of EGCG could not completely block the process of bone loss in ovariectomized rats. 2. At the dose of 10mg/kg/day, the effect of intraperitoneal injection may be better than that of gavage in preventing bone loss in female ovariectomized rats. 3. The expression of Sema4D in bone tissue of ovariectomized rats was higher than that of normal rats, and EGCG could decrease the expression of Sema4D in bone tissues of ovariectomized rats to some extent.
【學(xué)位授予單位】：蘇州大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R580

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 趙燕玲,潘子昂,王石麟,王虔,劉京萍,劉忠厚;中國原發(fā)性骨質(zhì)疏松癥流行病學(xué)[J];中國骨質(zhì)疏松雜志;1998年01期

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