本文選題：瘦素 + 瘦素受體　； 參考：《山東醫(yī)藥》2017年17期

【摘要】：目的探討腦室內(nèi)注射瘦素后大鼠迷走復合體瘦素受體(Ob-R)表達的變化。方法將SD大鼠24只隨機分為對照組和瘦素干預1 h組(A組)、3 h組(B組)、5 h組(C組),每組6只。給藥前1周行側(cè)腦室置管,置管1周后無麻醉狀態(tài)下對照組側(cè)腦室注射生理鹽水,A、B、C組側(cè)腦室注射瘦素,劑量均為3.5μg/μL。實驗結(jié)束后取腦組織,用HE染色進行迷走復合體定位,用免疫組化方法測定Ob-R在迷走復合體的表達。結(jié)果 A、B、C組Ob-R陽性細胞數(shù)分別為(12.49±1.66)、(12.54±2.16)、(12.89±1.86)個,對照組陽性細胞數(shù)為(5.66±1.04)個;A、B、C組Ob-R陽性細胞光密度積分分別為(28.41±3.60)、(26.18±6.17)、(24.56±4.14)分,對照組為(16.02±2.06)分;與對照組比較,A、B、C組Ob-R陽性細胞數(shù)、陽性細胞光密度積分增多(P均0.05)。A、B、C三組間Ob-R陽性細胞數(shù)、陽性細胞光密度積分比較差異無統(tǒng)計學意義(P均0.05)。結(jié)論瘦素中樞干預后可刺激迷走復合體上Ob-R的表達上調(diào),瘦素刺激時間與Ob-R水平無關(guān)。
[Abstract]:Objective to investigate the changes of leptin receptor (Ob-R-) expression in rat vagal complex after intracerebroventricular injection of leptin. Methods Twenty-four Sprague-Dawley rats were randomly divided into two groups: control group (n = 6) and leptin intervention group (n = 6). One week before administration, ventricular catheters were placed in the lateral ventricle. In the control group, the rats in the control group were injected with leptin at the dose of 3.5 渭 g / 渭 L under the condition of no anaesthesia. At the end of the experiment, the brain tissue was taken, and the localization of vagus complex was carried out by HE staining. The expression of Ob-R in the vagal complex was detected by immunohistochemical method. Results the number of Ob-R positive cells was 12.49 鹵1.66 鹵1.86 in group A and 5.66 鹵1.04 in control group, respectively, and the optical density score of Ob-R positive cells in group C was 28.41 鹵3.6026.18 鹵6.175.56 鹵4.14, and the number of Ob-R positive cells in group A and C was 16.02 鹵2.06, respectively. The number of Ob-R positive cells and the difference of optical density scores of positive cells between three groups were not statistically significant (P < 0.05). Conclusion leptin central intervention can up-regulate the expression of Ob-R on the vagal complex, and leptin stimulation time is not related to the level of Ob-R.
【作者單位】： 承德醫(yī)學院附屬醫(yī)院;承德醫(yī)學院解剖教研室;
【基金】：河北省科學技術(shù)研究與發(fā)展計劃項目(08276101D-20)
【分類號】：R965
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本文編號：1819786