本文選題：根尖周炎 + NF-κB�。� 參考：《大連醫(yī)科大學》2014年碩士論文

【摘要】：目的：通過建立大鼠根尖周炎模型，研究糞腸球菌對大鼠根尖周炎NF-κB p65表達的影響。 方法：選取健康雄性SD大鼠24只，使用小號球鉆于右側(cè)下頜第一磨牙近中窩行開髓術(shù)后，隨機分為A、B兩組，每組各12只。A組開髓后，髓腔內(nèi)封入PBS緩沖液小棉球，設為未封菌組；B組開髓后，髓腔內(nèi)封入糞腸球菌與PBS緩沖液調(diào)制的菌懸液小棉球，設為封菌組。兩組均使用進口玻璃離子粘固劑封閉髓腔。左側(cè)下頜第一磨牙為空白對照組。常規(guī)條件飼養(yǎng)，A、B兩組分別于術(shù)后1周、2周、3周、4周四個時間點隨機各取3只鼠，水合氯醛麻醉處死后，分離其下頜骨，用小號挖匙挖取右側(cè)下頜第一磨牙和左側(cè)下頜第一磨牙根尖區(qū)牙槽骨、牙周膜組織，之后對組織進行研磨，，提取、純化組織RNA，并逆轉(zhuǎn)錄成cDNA，采用實時熒光定量PCR (Real-time PCR)手段檢測各組NF-κB p65基因的表達水平并進行統(tǒng)計學分析。 結(jié)果： 1.健康空白對照組的NF-κB p65mRNA有少量表達，1周到4周A、B兩組各時間點的NF-κB p65mRNA的表達量均高于相應空白對照組，差異有統(tǒng)計學意義。（P0.05） 2．A、B兩組術(shù)后1周到2周NF-κB p65mRNA的表達水平逐漸增高，2周表達水平達最高峰，3周表達水平開始降低，4周表達水平繼續(xù)降低。四個實驗組中1周與4周之間的差異無統(tǒng)計學意義（P0.05），其余各組之間的差異均具有統(tǒng)計學意義（P0.05）。 3．術(shù)后2周、3周，B組NF-κB p65mRNA的表達量均高于A組，差異均具有統(tǒng)計學意義（P0.05）。 結(jié)論： 1．NF-κB p65基因的表達水平可能與根尖周炎的發(fā)生、發(fā)展密切相關(guān)，提示NF-κB p65參與了根尖周炎的炎癥反應和骨破壞。 2．NF-κB p65與糞腸球菌感染為主的根尖周炎密切相關(guān)，提示糞腸球菌存可能加重了根尖周組織的炎癥反應和骨破壞。
[Abstract]:Aim: to study the effect of Enterococcus faecalis on the expression of NF- 魏 B p65 in rat periapical periodontitis. Methods: Twenty-four male Sprague-Dawley (SD) rats were randomly divided into two groups: group A (n = 12) and group A (n = 12). PBS buffer was injected into the medullary cavity of 24 male Sprague-Dawley rats. After opening the pulp in group B of unsealed bacteria, the bacteria suspension of Enterococcus faecalis and PBS buffer was added into the medullary cavity. Both groups were treated with imported glass ionomer to seal the medullary cavity. The left mandibular first molar was a blank control group. Three rats were randomly selected from each group at the time points of 1 week, 2 weeks, 3 weeks and 4 days after routine feeding. The mandible was isolated after anesthesia with chloral hydrate. The alveolar bone and periodontal ligament of the right first molar and the left first molar of the right mandible were extracted with a small spoon, and then the tissue was ground and extracted. The expression level of NF- 魏 B p65 gene in each group was detected by real-time fluorescence quantitative PCR real-time PCR, and the expression level of NF- 魏 B p65 gene was analyzed statistically. Results: 1. The expression of NF- 魏 B p65mRNA in the healthy blank control group was significantly higher than that in the control group at each time point from 1 to 4 weeks. The difference was statistically significant (P 0.05). 2. The expression level of NF- 魏 B p65mRNA increased gradually from 1 week to 2 weeks after operation in both groups. The expression level of NF- 魏 B increased gradually at the end of 2 weeks. The expression level of NF- 魏 B began to decrease at the end of 3 weeks and the expression level of NF- 魏 B decreased at the 4th week. There was no significant difference between one week and four weeks in the four experimental groups, but the differences among the other groups were statistically significant. 3. The expression of NF- 魏 B p65mRNA in group B was significantly higher than that in group A at 2 weeks and 3 weeks after operation, and the difference was statistically significant (P 0.05). Conclusion: 1. The expression level of NF- 魏 B p65 gene may be closely related to the occurrence and development of periapical inflammation, suggesting that NF- 魏 B p65 is involved in the inflammatory response and bone destruction in periapical periodontitis. 2. NF- 魏 B p65 was closely related to periapical periodontitis with Enterococcus faecalis infection, suggesting that the presence of Enterococcus faecalis may aggravate inflammation and bone destruction in periapical tissue.
【學位授予單位】：大連醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R781.34

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