發(fā)布時間：2018-04-21 15:37

  本文選題：電容耦合電場 + 低頻脈沖電磁場��； 參考：《重慶大學》2014年碩士論文

【摘要】：隨著老齡化的加劇，骨質(zhì)疏松患者的人數(shù)也在逐年增加，目前已然躍居常見病、多發(fā)病的第七位。全世界骨質(zhì)疏松患者達2億之多，其引起的骨折越來越多，呈現(xiàn)上升趨勢。 骨質(zhì)疏松癥是以骨量減少、骨的顯微結(jié)構(gòu)退化為特征的，致使骨的脆性增加以及易于發(fā)生骨折的一種全身性骨骼疾病。骨質(zhì)疏松的類型共3種，，而絕經(jīng)后型屬于人數(shù)最多的一種。治療方法很多且各有優(yōu)劣。隨著生物醫(yī)學工程的發(fā)展，電磁場治療的諸多優(yōu)點被人們逐步的認識，其中低頻脈沖磁場與電容耦合電場就是治療骨質(zhì)疏松的有效的方法。 本課題采用PEMFs與CCPEF配合治療，通過卵巢切除法建立骨質(zhì)疏松動物模型來驗證該治療方法的效果。探討該方法的治療效果與兩者單一治療的效果，比較何種方法更佳。 動物選取與分組：選用3月齡大小的雌性SD大鼠，體重為250±20g，50只，隨機分成5組：Blank組、Contrast組、PEMFs組、CCPEF組、PEMFs+CCPEF組。 方案：Blank組進行假手術(shù)，其余4組制作動物模型。模型完成后4周進行治療，Blank組與Contrast組：不進行任何刺激；PEMFs組：將大鼠放入自行設(shè)計的飼養(yǎng)籠內(nèi)，暴露在頻率為15Hz，強度為0.4mT的低頻脈沖磁場中，8小時/天，連續(xù)治療12周；CCPEF組：乙醚麻醉后，將大鼠放于自制的耦合電容平行板之間，刺激頻率為1.5MHz，強度為20Vpp，每天一次，每次20分鐘，每周5次，連續(xù)治療55次。PEMFs+CCPEF組：首先進行PEMFs暴露治療，方案跟PEMFs組一樣；然后進行CCPEF暴露治療，治療方案跟CCPEF組一樣，即兩者配合治療。 采樣數(shù)據(jù)：身體狀況觀察及體重測量、尿液的收集、血液的采集、摘取臟器并稱重、生物力學性能的測定。 結(jié)果：從體重變化、子宮系數(shù)、生化指標、骨最大應(yīng)力數(shù)據(jù)參數(shù)中，Contrast組與Blank組相比較，均具有顯著的變化，P 0.01，說明動物模型制作成功；PEMFs組、CCPEF組、PEMFs+CCPEF組這3組與Contrast組相比較，均具有一定的差異，P 0.05，說明PEMFs、CCPEF、PEMFs+CCPEF這3中治療方式均具有治療骨質(zhì)疏松的作用；PEMFs組與CCPEF組比較，無顯著差異，說明低頻脈沖電磁場與電容耦合電場治療效果差不多，各有優(yōu)缺點；PEMFs+CCPEF組與PEMFs組、CCPEF組比較，除了Ca、P、ALP參數(shù)數(shù)據(jù)外，其他參數(shù)均具有一定的變化，P 0.05，說明電容耦合電場配合低頻脈沖電磁場的治療方式比它們單一的治療方式效果更好。
[Abstract]:With the aggravation of aging, the number of osteoporosis patients is increasing year by year. There are more than 200 million osteoporosis patients in the world, and more and more fractures are caused by it. Osteoporosis is a systemic bone disease characterized by the reduction of bone mass and the degeneration of bone microstructure, which increases the fragility of bone and is prone to fracture. There are three types of osteoporosis, and the postmenopausal type is the most numerous. There are many methods of treatment and each has its own advantages and disadvantages. With the development of biomedical engineering, many advantages of electromagnetic field therapy have been gradually recognized, among which low-frequency pulsed magnetic field and capacitive coupling electric field is an effective method for the treatment of osteoporosis. In this study, PEMFs combined with CCPEF was used to establish an animal model of osteoporosis by ovariectomy. To explore the therapeutic effect of this method and the effect of single treatment, compared with which method is better. Animal selection and grouping: 50 female Sprague-Dawley rats aged 3 months, weighing 250 鹵20 g / g, were randomly divided into 5 groups: control group (n = 5): Contrast group (n = 5): CCPEF group (n = 10) and PEMFs group (n = 50). Plan: Blank group for sham-operation, the other four groups to make animal models. Four weeks after the completion of the model, the two groups were treated with Contrast: the rats were exposed to low frequency pulsed magnetic field (0.4mT) with frequency of 15 Hz and intensity of 0.4mT for 8 hours per day without any stimulation, and the rats were placed in a self-designed cage, and the rats were exposed to a low frequency pulsed magnetic field with a frequency of 15 Hz and an intensity of 0.4mT for 8 hours per day. After 12 weeks of continuous treatment with CCPEF, the rats were placed between the self-made coupling capacitor parallel plates, the stimulation frequency was 1.5 MHz, the intensity was 20 Vpp. once a day, 20 minutes a week, 5 times a week. Continuous treatment for 55 times. PEMFs CCPEF group: first, PEMFs exposure treatment, the same scheme as PEMFs group, then CCPEF exposure therapy, the same treatment as CCPEF group, that is, the combination of both groups. Sampling data: body condition observation and weight measurement, urine collection, blood collection, organ extraction and weighing, biomechanical performance measurement. Results: compared with Blank group, the weight change, uterine coefficient, biochemical index and bone maximum stress data of Contrast group were significantly different from those of Blank group (P 0.01), which indicated that the three groups of CCPEF group were compared with Contrast group. There was no significant difference between PEMFs group and CCPEF group, which indicated that the effect of PEMFs CCPEFF CCPEF on osteoporosis was similar to that of CCPEF group, indicating that the effect of low frequency pulsed electromagnetic field was similar to that of capacitive coupled electric field, and that there was no significant difference between PEMFs group and CCPEF group in the treatment of Osteoporosis, which indicated that there was no significant difference between PEMFs group and CCPEF group. Compared with the PEMFs group, the other parameters of PEMFs CCPEF group have some changes (P 0.05), which indicates that the capacitive coupled electric field combined with low frequency pulse electromagnetic field is more effective than their single treatment method.
【學位授予單位】：重慶大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R580

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