頂羽菊提取物對鱗翅目幼蟲的殺蟲活性研究
發(fā)布時間:2018-04-05 05:03
本文選題:頂羽菊 切入點:提取物 出處:《西南農(nóng)業(yè)學(xué)報》2015年03期
【摘要】:探明頂羽菊提取物對鱗翅目幼蟲的殺蟲活性,可為利用該植物研制新型植物源殺蟲劑提供線索和理論依據(jù)。本研究采用蟲體浸漬法和夾毒葉碟法測定了頂羽菊提取物對粘蟲的作用方式以及對小菜蛾、菜粉蝶等鱗翅目幼蟲的觸殺活性。結(jié)果表明,頂羽菊盛花期全株乙酸乙酯粗提物對粘蟲5齡幼蟲具有很強的觸殺作用,稀釋5倍液的校正死亡率為80%;此粗提物之石油醚萃取物對粘蟲5齡幼蟲具有很強的觸殺作用,稀釋5倍液的校正死亡率為86.88%,其毒力回歸方程為Y=2.5719+2.9107X,致死中濃度LC50值為6.83 g浸膏/L。石油醚萃取物對粘蟲的作用方式表現(xiàn)為很強的觸殺作用(稀釋5倍液的校正死亡率為86.88%)、一定的胃毒作用(在96.4 g浸膏/L濃度下的校正死亡率為21.28%)、較強的生長發(fā)育抑制作用(處理后2、3和4 d的生長發(fā)育抑制率分別為60.61%、51.94%和43.53%)、較強的麻醉作用(1 h內(nèi)的麻醉率為53%,麻醉作用的回歸方程為Y=4.2337+1.6839X,蘇醒中時RT50=2.85 h)、無拒食和內(nèi)吸以及熏蒸作用、無殺卵和殺蛹作用。石油醚萃取物對小菜蛾、菜粉蝶、大菜粉蝶和云斑粉蝶幼蟲具有很強的觸殺作用,稀釋5倍液(小菜蛾4倍液)的校正死亡率分別為85%、100%、93.33%和100%;對棉鈴蟲幼蟲具有較強的觸殺作用,稀釋5倍液的校正死亡率為62.5%。
[Abstract]:Proven top feather chrysanthemum extract insecticidal activity against lepidopteran larvae, provide clues and theoretical basis for the development of new plant botanical insecticides. The research and determination of the toxic leaf disc clamping effect of top feather chrysanthemum extracts against Mythimnaseparata and larvae of diamondback moth by impregnation method, and other lepidopteran larvae of Pieris rapae contact activity. The results showed that the top feather Jusheng flowering plant ethyl acetate extract against 5 instar larvae with strong contact effect, the corrected mortality of 5 times dilution was 80%; the crude extracts of petroleum ether extract against 5 instar larvae with strong contact effect the corrected mortality of 5 times dilution was 86.88%, the regression equation was Y=2.5719+2.9107X, the median lethal concentration LC50 value was 6.83 g /L. extract of petroleum ether extract against strong contact toxicity (5 times dilution corrected death rate 86.88%), certain stomach toxicity (corrected mortality at 96.4 g for 21.28% /L extract concentration), strong growth inhibition activity (the inhibition rate was 60.61% 2,3 and 4 d after treatment with 51.94% and 43.53% growth and development), the anesthetic effect strong (1 h in the regression equation of rate of anesthesia the anesthesia effect was 53%, the recovery is Y=4.2337+1.6839X, RT50=2.85 h), when no antifeedant and absorption and fumigation effect, no ovicidal and pupa killing effect. Petroleum ether extracts on Pieris rapae, Plutella xylostella, Pieris brassicae and Batocera Pieris rapae with strong contact effect, 5 times dilution (diamondback moth 4 times liquid) corrected mortality were 85%, 100%, 93.33% and 100%; has strong contact effect on cotton bollworm, the corrected mortality of 5 times dilution was 62.5%.
【作者單位】: 甘肅農(nóng)業(yè)大學(xué)草業(yè)學(xué)院農(nóng)藥學(xué)系;甘肅省農(nóng)業(yè)科學(xué)院植物保護(hù)研究所;
【基金】:甘肅省農(nóng)業(yè)生物技術(shù)研究與應(yīng)用開發(fā)項目(GNSW-2005-04)
【分類號】:S482.39
【參考文獻(xiàn)】
相關(guān)期刊論文 前10條
1 張新瑞;胡冠芳;劉敏艷;李玉奇;余海濤;;紅蓼提取物殺蟲活性及其對粘蟲的作用方式[J];草地學(xué)報;2009年03期
2 王洪鳳;孫明清;劉順;張鳳國;秦秋菊;何運轉(zhuǎn);;,
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