本文選題：黃連阿膠湯加減　切入點(diǎn)：糖尿病大鼠　出處：《廣州中醫(yī)藥大學(xué)》2014年碩士論文

【摘要】：目的： 研究傷寒論經(jīng)方治療糖尿病的療效及機(jī)理。運(yùn)用中醫(yī)藥基礎(chǔ)理論,結(jié)合現(xiàn)代醫(yī)學(xué)實(shí)驗(yàn)技術(shù),從動物實(shí)驗(yàn)研究方面,觀察黃連阿膠湯加減對糖尿病大鼠血糖、甘油三脂、膽固醇、胰島素、胰島素抵抗指數(shù)、瘦素等的影響,分析這些指標(biāo)在糖尿病中的變化及意義,從而評價黃連阿膠湯加減的降糖療效及其作用機(jī)理,進(jìn)而促進(jìn)中藥復(fù)方制劑治療糖尿病研究的廣度和深度,也將為中醫(yī)藥治療糖尿病臨床療效的評價和推廣提供一個有效的平臺。 方法： 探討中西醫(yī)學(xué)對糖尿病病因及發(fā)病機(jī)制的認(rèn)識,簡述中西醫(yī)藥物對糖尿病的治療機(jī)理,對近年來黃連阿膠湯臨床應(yīng)用及實(shí)驗(yàn)研究進(jìn)展進(jìn)行概況性回顧,總結(jié)歷代醫(yī)家對黃連阿膠湯的認(rèn)識,從而提出“苦酸制甜”法是建立本研究方劑的基礎(chǔ)。采用SPF級SD大鼠50只,雌雄各半,隨機(jī)分為中藥組、格列齊特組、模型組、空白組,前3組每組14只,空白組8只。造模組使用STZ按55mg/kg腹腔內(nèi)注射,造模成功后,中藥組按30g/kg、格列齊特組按20mg/kg計算灌胃,空白組、模型組分別灌服等容積蒸餾水,均每日一次,一共灌胃2周。實(shí)驗(yàn)期間,各組均予普通飼料。灌胃2周后麻醉大鼠,取血液樣本,最后用脫臼法處死大鼠。實(shí)驗(yàn)一,研究黃連阿膠湯加減對糖尿病大鼠血糖的影響：評價造模成功與否的隨機(jī)血糖采用剪尾取血,用快速微量血糖儀進(jìn)行檢測；治療后空腹血糖的檢測采用腹主動脈取血,用全自動生化分析儀檢測。實(shí)驗(yàn)二,研究黃連阿膠湯加減對糖尿病大鼠血脂、胰島素、胰島素抵抗指數(shù)、瘦素水平的影響：腹主動脈采血后,甘油三脂、膽固醇用全自動生化分析儀檢測,胰島素、瘦素采用RayBio試劑盒檢測,胰島素抵抗指數(shù)公式：HOMA-IR=FPG (mmol/L)×FINS(mU/L)/22.5,計算后取自然對數(shù)。 結(jié)果： 1.大鼠造模組治療后空腹血糖與空白組相比普遍升高,其中中藥組、模型組分別與空白組相比差異具有顯著性意義(p0.05),而格列齊特組與空白組相比差異無統(tǒng)計學(xué)意義(p0.05)。格列齊特組與模型組相比差異具有顯著性意義(p0.05)。中藥組雖與模型組相比差異無統(tǒng)計學(xué)意義(p0.05),但兩組均值差約等于-1。 2.各組大鼠甘油三脂、膽固醇、胰島素、胰島素抵抗指數(shù)、瘦素組間差異無統(tǒng)計學(xué)意義。 結(jié)論： 格列齊特降糖療效顯著。中藥組雖與模型組相比差異無統(tǒng)計學(xué)意義,但兩組均值差約等于-1,同時觀察中藥組大鼠糖尿病各癥狀較模型組大鼠為輕,總體情況較前好轉(zhuǎn),推測中藥組可能存在一定的降糖療效。中藥組降糖療效不理想可能與給藥時間過短、樣本量少、未能方證對應(yīng)、藥劑制取與灌服藥物方法不完善等相關(guān)。各組血脂、胰島素、胰島素抵抗指數(shù)、瘦素指標(biāo)比較未見明顯差異,推測與造模未能誘導(dǎo)明顯的胰島素抵抗相關(guān),因此無法深入探討該方降糖機(jī)制是否與胰島素抵抗相關(guān)。
[Abstract]:Objective:To study the curative effect and mechanism of treating diabetes mellitus with Shufangxuan Meridian prescription.The effects of Huanglian Ejiao decoction on blood glucose, triglyceride, cholesterol, insulin, insulin, insulin resistance index, leptin and so on in diabetic rats were observed by using the basic theory of traditional Chinese medicine and modern medical experimental technology.The changes and significance of these indexes in diabetes mellitus were analyzed to evaluate the hypoglycemic effect of Huanglian Ejiao decoction and its mechanism, and to promote the breadth and depth of the research on the treatment of diabetes mellitus with traditional Chinese medicine compound preparation.It will also provide an effective platform for evaluating and popularizing the clinical efficacy of traditional Chinese medicine in the treatment of diabetes.Methods:To explore the etiology and pathogenesis of diabetes in Chinese and western medicine, to summarize the therapeutic mechanism of traditional Chinese and western medicine on diabetes, and to review the clinical application and experimental research progress of Huanglian Ejiao decoction in recent years.The understanding of Huanglian Ejiao decoction was summarized, and the method of "bitter acid making sweet" was put forward.Fifty SD rats of SPF grade were randomly divided into Chinese medicine group, gliclazide group, model group, blank group, 14 rats in each group, and 8 rats in blank group.The model group was injected intraperitoneally with STZ and 55mg/kg. After the model was successfully made, the Chinese medicine group was given 30 g / kg, the gliclazide group was treated with 20mg/kg, and the blank group and the model group were given equal volume distilled water once a day for 2 weeks.During the experiment, all groups were given common feed.The anesthetized rats were anesthetized for 2 weeks, blood samples were taken, and the rats were killed by dislocated method.Experiment 1, study the effect of Huanglian Ejiao decoction on blood sugar in diabetic rats: the random blood sugar used to evaluate the success of the model was to take blood from the tail and detect it with a rapid micro-blood glucose meter, and the fasting blood glucose was measured by abdominal aorta after treatment.Automatic biochemical analyzer was used to detect.The second experiment was to study the effects of Huanglian Ejiao decoction on blood lipids, insulin, insulin resistance index and leptin level in diabetic rats: after blood collection from abdominal aorta, triglyceride and cholesterol were detected by automatic biochemical analyzer.Leptin was detected by RayBio kit. The insulin resistance index formula was: HOMA-IRFPG / mmol / L) 脳 FINSU / L / L = 22.5. The natural logarithm was calculated.Results:1.After treatment, fasting blood glucose in the model group was generally higher than that in the blank group. There was significant difference between the traditional Chinese medicine group and the model group compared with the blank group, but there was no significant difference between the gliclazide group and the blank group.There was significant difference between Gliclazide group and model group (P 0.05).Although there was no significant difference between the traditional Chinese medicine group and the model group, the difference between the two groups was about-1.2.There was no significant difference in triglyceride, cholesterol, insulin, insulin resistance index and leptin.Conclusion:Gliclazide has a remarkable effect on reducing glucose.Although there was no significant difference between the Chinese medicine group and the model group, the difference between the two groups was about -1.At the same time, the symptoms of diabetes in the traditional Chinese medicine group were lighter than those in the model group, and the overall situation was improved.It is speculated that the Chinese medicine group may have some hypoglycemic effect.The hypoglycemic effect of traditional Chinese medicine group may be related to the short time of administration, the small sample size, the lack of prescription syndrome, the preparation of medicine and the imperfection of the method of drug administration.There was no significant difference in serum lipids, insulin, insulin resistance index and leptin index in each group. It was speculated that there was no obvious relationship between insulin resistance and insulin resistance induced by model making, so it was impossible to explore whether the hypoglycemic mechanism of the prescription was related to insulin resistance.
【學(xué)位授予單位】：廣州中醫(yī)藥大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R285.5

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 蘇曉艷;方朝暉;;瘦素及瘦素抵抗與肥胖、2型糖尿病的關(guān)系研究進(jìn)展[J];安徽醫(yī)藥;2009年01期

2 楊桂染;李淑貞;劉娜;楊艷梅;王曉宇;;加味黃連阿膠湯對順鉑腎毒性大鼠TGF-β_1表達(dá)的影響[J];浙江中醫(yī)藥大學(xué)學(xué)報;2009年06期

3 鄭建洵;;2型糖尿病患者瘦素水平及與胰島素抵抗的關(guān)系[J];福建醫(yī)藥雜志;2012年01期

4 宿廷敏;王敏娟;阮時寶;;白術(shù)的化學(xué)成分及藥理作用研究概述[J];貴陽學(xué)院學(xué)報(自然科學(xué)版);2008年02期

5 劉得華;;黃連阿膠湯加減治療陰虛熱盛型糖尿病的臨床研究[J];光明中醫(yī);2006年04期

6 張小軍;;黃連阿膠湯治療發(fā)燒臨床解讀[J];光明中醫(yī);2011年11期

7 張秉全;馬建;徐洪濤;;瘦素(Leptin)的研究進(jìn)展[J];黑龍江醫(yī)藥;2008年02期

8 張宗如;黃連阿膠湯在婦科的應(yīng)用[J];黑龍江中醫(yī)藥;1999年06期

9 李俊松;張艷秋;潘f ;;黃連阿膠湯加減治療陰虛火旺型頑固性失眠36例[J];黑龍江中醫(yī)藥;2011年03期

10 張立;加味黃連阿膠湯治療Ⅱ型糖尿病45例[J];湖南中醫(yī)藥導(dǎo)報;2000年06期

，

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