參芪泄?jié)犸媽ο汆堰誓I間質纖維化模型大鼠腎組織MMP-9、TIMP-1基因表達的影響
發(fā)布時間：2018-04-03 01:34
本文選題：參芪泄?jié)犸?/strong> 切入點：基質金屬蛋白酶 出處：《遼寧中醫(yī)雜志》2015年01期
【摘要】：目的:觀察參芪泄?jié)犸媽β阅I衰大鼠腎組織基質金屬蛋白酶9(MMP-9)、基質金屬蛋白酶抑制劑1(TIMP-1)基因表達的影響。方法:采用Yokozawa法復制慢性腎衰大鼠模型,隨機分為參芪泄?jié)犸嫺邉┝拷M、常規(guī)劑量組、氯沙坦組、模型組及空白組,高劑量組、常規(guī)劑量組、氯沙坦組分別予參芪泄?jié)犸嬎逡?0 m L/(kg·d)、參芪泄?jié)犸?0 m L/(kg·d)、氯沙坦9 mg/(kg·d),模型組與空白組大鼠均予純凈水10 m L/(kg·d),連續(xù)給藥8周,全部大鼠麻醉后腹腔采血,取左腎,ELLISA法測量血肌酐(Scr)、尿素氮(BUN)、丙氨酸轉氨酶(ALT)并采用熒光定量PCR檢測腎臟MMP-9、TIMP-1 mRNA的表達。結果:1高劑量組Scr水平明顯低于常規(guī)劑量組、氯沙坦組及模型組;常規(guī)劑量組與氯沙坦組無明顯差別,且均低于模型組;2高劑量組MMP-9 mRNA表達水平明顯高于常規(guī)劑量組、氯沙坦組及模型組;常規(guī)劑量組與氯沙坦組無明顯差別,且均高于模型組;高劑量組TIMP-1 mRNA表達水平明顯低于常規(guī)劑量組、氯沙坦組及模型組;常規(guī)劑量組與氯沙坦組無明顯差別,且均低于模型組;3各組ALT水平較空白組比較均無顯著差別。結論:參芪泄?jié)犸嬁赏ㄟ^調節(jié)MMP-9及TIMP-1表達來緩解慢性腎衰腎臟功能的惡化。
[Abstract]:Aim: to observe the effect of Shenqi Xiezhuo decoction (Shenqi Xiezhuo decoction) on the expression of matrix metalloproteinase 9 (MMP-9) and matrix metalloproteinase inhibitor 1 (TIMP 1) in renal tissue of rats with chronic renal failure (CRF).Methods: the rat model of chronic renal failure was established by Yokozawa method. The rats were randomly divided into three groups: high dose group, routine dose group, losartan group, model group and blank group, high dose group and routine dose group.姘矙鍧︾粍鍒嗗埆浜堝弬鑺硠嫻婇ギ姘寸厧娑，
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