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  本文選題：七氟烷　切入點：糖尿病　出處：《福建醫(yī)科大學》2014年碩士論文

【摘要】：目的探討七氟烷不同吸入麻醉時間對糖尿病相關認知降低(Diabetes-associated cognitive decline,DACD)老年大鼠認知功能的影響,并從海馬遞質(zhì)谷氨酸(Glutamic acid,Glu)及γ-氨基丁酸(Gamma aminobutyric acid,GABA)含量及Glu"GABA比值的變化初步探討其可能的作用機制,為臨床上DACD老年患者合理選用麻醉藥物和麻醉方法提供參考。方法選擇90只清潔級SD老年大鼠,隨機分為3組(n=30):對照組(C組):采用普通飼料飼養(yǎng)8周后,腹腔注射檸檬酸緩沖液,繼續(xù)原普通飼料飼養(yǎng)7周,期間自由飲水;糖尿病組(D組):采用高糖高脂飼料飼養(yǎng)8周后,腹腔注射檸檬酸緩沖液稀釋的鏈脲佐菌素(Streptozotocin,STZ)后繼續(xù)原高糖高脂飼養(yǎng)方案7周,期間自由飲水,建立DACD老年大鼠模型;七氟烷組(DS組):先以D組方案構(gòu)建DACD模型,然后接受1.5%七氟烷吸入麻醉,按時間長短分成3個亞組:DS1組(1小時,n=10)、DS2組(2小時,n=10)、DS3組(3小時,n=10);C組和D組則在同等條件下采用純氧吸入,根據(jù)吸入純氧時間的不同,分別將C組分為3個亞組:C1(1小時,n=10)、C2(2小時,n=10)、C3(3小時,n=10);D組分為3個亞組:D1(1小時,n=10)、D2(2小時,n=10)、D3組(3小時,n=10)。待七氟烷吸入麻醉或單純吸入純氧處理后1天、5天,再次測定C組,D組、DS組各亞組大鼠的認知功能水平,并與處理前進行對比,判斷其認知功能的改變。而后采用斷頭取腦法提取大鼠海馬CA1區(qū)組織,測定海馬CA1區(qū)中Glu與GABA含量及Glu/GABA比值變化。結(jié)果1、采用高糖高脂喂養(yǎng)復合少量STZ腹腔注射法成功建立T2DM老年大鼠模型;2、處理前認知水平比較,D組各亞組、DS組各亞組均較C組各亞組降低,差異有統(tǒng)計學意義(P0.05);處理后1天認知水平比較,DS3組均較D3、C3降低,差異有統(tǒng)計學意義(P0.05);3、處理后1天比較,DS3組Glu含量降低、GABA含量升高、Glu/GABA比值降低,分別與C3、D3組比較差異均有統(tǒng)計學意義(P0.05)。結(jié)論DACD老年大鼠采用1.5%七氟烷吸入麻醉超過3小時及以上,短期內(nèi)可出現(xiàn)認知功能降低,其作用機制與降低海馬CA1區(qū)中Glu含量,同時增加GABA含量有關。
[Abstract]:Objective to investigate the effects of different inhalation anesthetic time of sevoflurane on the cognitive function of Diabetes-associated cognitive declination in aged rats. The possible mechanism of Glutamic acid (Glutamic acid Glua), Gamma aminobutyric acidine GABA (緯 -aminobutyric acid) and the ratio of Glu "GABA" in hippocampus were also discussed. Methods 90 old SD rats of clean grade were randomly divided into 3 groups: control group C: normal diet for 8 weeks. Citric acid buffer solution was injected intraperitoneally and continued to be fed with normal diet for 7 weeks, during which drinking water was free. Group D of diabetes mellitus was fed with high-sugar and high-fat diet for 8 weeks. After intraperitoneal injection of streptozotocin (STZ) diluted with citric acid buffer solution, we continued the original high-sugar and high-fat feeding scheme for 7 weeks, during which the aged DACD rat model was established by drinking water freely, while in the sevoflurane group, the DACD model was established in the DS group: group D was used to construct the DACD model. They were then anesthetized with 1.5% sevoflurane and were divided into three subgroups according to the length of time: one hour or one hour, one hour, one hour, one hour, one hour, one hour, one hour, one hour, one hour, one hour, one hour, three hours of pure oxygen inhalation in group C and group D, according to the time of pure oxygen inhalation. Group C was divided into 3 subgroups: 1 h, 1 hour, 1 hour, 1 hour, 1 hour, 1 hour, 1 hour, 1 hour, 1 hour, 1 hour, 10 hour, 10 hour, 3 hours, respectively. Group D was divided into 3 subgroups. Group D was divided into 3 subgroups. Group D was divided into 3 subgroups. After inhaling sevoflurane anesthesia or inhaling pure oxygen for 1 day and 5 days after treatment, group D was divided into three subgroups: group D 1: 1 and group D 2: 1 h after inhalation of sevoflurane or inhalation of pure oxygen alone, the rats were treated with sevoflurane or inhaled pure oxygen for 1 day and 5 days after treatment. The cognitive function of rats in group C and group D were measured again, and compared with those before treatment, the changes of cognitive function were judged. Then the CA1 tissue of hippocampus was extracted by the method of head cut. The content of Glu and GABA and the ratio of Glu/GABA to Glu were measured in the CA1 region of hippocampus. Results 1 the senile rat model of T2DM was successfully established by high glucose and high fat feeding combined with a small amount of STZ intraperitoneal injection. The cognitive level before treatment was compared between the D group and the DS group. Group C was lower than group C. The cognitive level of DS3 group was lower than that of D3C3 group on the first day after treatment, the difference was statistically significant (P 0.05), and the Glu content of DS3 group was lower than that of D3GA-GABA group on the 1st day after treatment, the ratio of Glu-P / GABA to Glu-P / GABA ratio in DS3 group was higher than that in DS3 group. Conclusion the cognitive function of aged DACD rats after inhalation anesthesia of 1.5% sevoflurane for more than 3 hours could be decreased in a short period of time, and its mechanism might be related to the decrease of Glu content in the CA1 area of hippocampus. At the same time, the content of GABA was increased.
【學位授予單位】：福建醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R614

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相關期刊論文 前1條

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本文編號：1691032