本文選題：骨碎補(bǔ)總黃酮　切入點(diǎn)：PMOP　出處：《河南中醫(yī)學(xué)院》2014年碩士論文

【摘要】：目的：隨著社會科技的進(jìn)步，特別是醫(yī)學(xué)臨床技術(shù)和科研力量的提高，人類整體壽命延長，世界上大多數(shù)國家老年人人數(shù)增多，絕經(jīng)后骨質(zhì)疏松癥發(fā)病率逐年的快速增長，已經(jīng)成為影響老年人身體健康的主要疾病之一，同時也帶來了沉重的社會負(fù)擔(dān)。本研究以骨碎補(bǔ)總黃酮為研究對象，以去勢SD大鼠為模型載體，模擬人類雌激素分泌減少后身體機(jī)能，觀測骨碎補(bǔ)總黃酮對去勢大鼠的生化指標(biāo)、骨密度及病理組織切片、骨組織中端粒酶的表達(dá)的影響，探討骨碎補(bǔ)總黃酮在治療絕經(jīng)后骨質(zhì)疏松癥的進(jìn)程中可能的作用機(jī)制。 方法：雌性SD大鼠60只，90天齡，體重控制在220—240g，隨機(jī)分成6組，分別為假手術(shù)組（SHAM）、模型組（OVX）、骨碎補(bǔ)總黃酮小劑量組（G小）、骨碎補(bǔ)總黃酮中劑量組（G中）、骨碎補(bǔ)總黃酮大劑量組（G大）、雌二醇（補(bǔ)佳樂）組(E2)各10只，造摸用背側(cè)雙切口卵巢摘除術(shù)；用蒸餾水或者配制藥物溶液灌胃3個月，經(jīng)麻醉后腹主動脈取血，截取雙側(cè)股骨。血液分離取血清測生化指標(biāo)血清鈣（Ca）、磷（P）、骨堿性磷酸酶（B-ALP）；右股骨端用于骨密度測定，再經(jīng)HE染色，在光鏡下觀察病理組織學(xué)切片；左股骨端測定端粒酶在骨組織中的表達(dá)情況。 結(jié)果：在本實驗研究中，與SHAM相比，OVX生化指標(biāo)檢測、骨密度及端粒酶表達(dá)值差異有顯著統(tǒng)計學(xué)意義（P 0.01），G高、E2無統(tǒng)計學(xué)意義（P0.05）；與OVX組相比，用藥各組血鈣、血磷、含量升高，骨堿性磷酸酶降低，其中G高及E2顯著統(tǒng)計學(xué)意義（P0.01），，G低及G中有統(tǒng)計學(xué)意義（P0.05），G高與E2相比，無統(tǒng)計學(xué)意義（P0.05）；病理組織切片觀察到骨小梁假手術(shù)組粗細(xì)均勻，分布稠密，可見各種骨細(xì)胞，G高及E2變化不大，G低及G中可見骨小梁變細(xì)，不均勻，以及邊緣鋸齒樣改變，OVX可見骨小梁斷裂、溶解以及融合的脂肪空泡等典型絕經(jīng)后骨質(zhì)疏松癥改變。 結(jié)論：骨碎補(bǔ)總黃酮能提高去勢大鼠端粒酶的表達(dá)量，表明其可以影響骨組織中端粒酶的表達(dá)，從而改善絕經(jīng)后骨質(zhì)疏松癥。
[Abstract]:Objective: with the progress of social science and technology, especially the improvement of medical clinical technology and scientific research strength, the whole life span of human being has been prolonged, the number of elderly people in most countries in the world has increased, and the incidence of postmenopausal osteoporosis has been increasing rapidly year by year. It has become one of the major diseases affecting the health of the elderly, and it also brings a heavy social burden. In this study, the total flavonoids of Fructus fragmentae were used as the research object and the ovariectomized SD rats as the model carrier. The effects of total flavonoids on the biochemical indexes, bone mineral density (BMD) and the expression of telomerase in bone tissue of ovariectomized rats were observed. To explore the possible mechanism of total flavonoids in the treatment of postmenopausal osteoporosis. Methods: sixty female Sprague-Dawley rats (90 days old) were divided into 6 groups randomly and their body weight was controlled at 220-240 g. There were 10 rats in the model group, 10 in the small dose group of flavonoids, 10 in the middle dose group, 10 in the large dose group, 10 in the estradiol group, 10 in the middle dose group, 10 in the total flavonoid group, 10 in the sham-operated group, 10 in the model group, 10 in the small dose group, 10 in the middle dose group, 10 in the large dose group and 10 in the estradiol group, respectively. Ovariectomies were performed with bilateral dorsal incision, and the abdominal aorta was taken after anesthesia for 3 months by gavage with distilled water or prepared drug solution. Bilateral femurs were cut off. Blood samples were taken to measure serum biochemical indexes, such as calcium, phosphophosphatase, bone alkaline phosphatase B-ALP, right femur for bone mineral density determination, HE staining, and histopathological sections under light microscope. The expression of telomerase in bone tissue was measured at the left femur. Results: compared with SHAM, there were significant differences in bone mineral density (BMD) and telomerase expression between the two groups. The level of bone alkaline phosphatase (BALP) was significantly lower than that of E2, and there was no significant difference between P0.05G and E2. The histopathological sections showed that the bone trabecular sham-operated group was uniform in thickness and dense in distribution. It can be seen that the changes of G and E _ 2 in all kinds of bone cells are not obvious, and the trabeculae become thinner and uneven in G, and the bone trabeculae break down, the adipose vacuoles dissolved and fused, and so on. The changes of bone trabeculae in OVX are typical postmenopausal osteoporosis. Conclusion: total flavonoids can increase the expression of telomerase in ovariectomized rats, indicating that it can affect the expression of telomerase in bone tissue and improve postmenopausal osteoporosis.
【學(xué)位授予單位】：河南中醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R285.5

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