本文選題：促性腺激素抑制激素　切入點(diǎn)：促性腺激素釋放激素　出處：《山東醫(yī)藥》2017年27期

【摘要】：目的觀察側(cè)腦室微量注射促性腺激素抑制激素(Gn IH)后雄性大鼠血漿促性腺激素釋放激素(GnRH)、卵泡刺激素(FSH)水平變化,及下丘腦組織中阿片促黑素細(xì)胞皮質(zhì)素原(POMC)蛋白的表達(dá)變化。方法雄性SD大鼠60只,隨機(jī)分為實(shí)驗(yàn)組和對照組各30只。10%水合氯醛腹腔注射麻醉,實(shí)驗(yàn)組于大鼠側(cè)腦室一次性注射Gn IH 5μL,于5 min內(nèi)注射完畢,留針10 min。對照組給予等量生理鹽水。兩組分別于注射后60、120、240min各取10只大鼠心房取血,采用ELISA法檢測血漿GnRH、FSH;心房取血后斷頭處死大鼠,取下丘腦組織,采用Western blotting法檢測下丘腦組織中的POMC蛋白。結(jié)果實(shí)驗(yàn)組注射后60、120、240 min血漿GnRH、FSH水平均低于對照組(P均0.05),實(shí)驗(yàn)組內(nèi)注射后不同時點(diǎn)差異無統(tǒng)計(jì)學(xué)意義。實(shí)驗(yàn)組注射后60、120、240 min下丘腦組織中POMC蛋白相對表達(dá)量低于對照組,且實(shí)驗(yàn)組注射后240 min下丘腦組織中POMC蛋白相對表達(dá)量低于注射后60、120 min(P均0.05)。結(jié)論側(cè)腦室微量注射Gn IH后雄性大鼠血漿GnRH、FSH水平降低,下丘腦組織中POMC蛋白表達(dá)下調(diào)。Gn IH對下丘腦-垂體-性腺軸有一定調(diào)節(jié)作用,其機(jī)制可能與抑制POMC神經(jīng)元功能有關(guān)。
[Abstract]:Objective to observe the changes of plasma gonadotropin releasing hormone (GnRH) and follicle stimulating hormone (FSHs) levels in male rats after microinjection of gonadotropin inhibitory hormone (Gn IH) into lateral ventricle. Methods 60 male Sprague-Dawley rats were randomly divided into experimental group (n = 30) and control group (n = 30) by intraperitoneal injection of chloral hydrate. The rats in the experimental group were injected with GnIH 5 渭 L in the lateral ventricle at one time, and the rats were injected within 5 min with the needle for 10 minutes. The control group was given the same amount of normal saline. The atrial blood was taken from 10 rats in each of the two groups after the injection of 60120240min. The plasma GnRHH FSHs were detected by ELISA method, the rats were killed by decapitation and hypothalamus tissue were taken out. Results the levels of POMC in plasma of the experimental group at 60120240 min after injection were lower than those of the control group (P < 0.05), but there was no significant difference between the two groups at different time points after injection. There was no significant difference between the experimental group and the control group at different time points after injection. The relative expression of POMC protein in hypothalamus was lower than that in control group. The relative expression of POMC protein in hypothalamus of the experimental group was lower than that of the control group at 60120 min(P after injection. Conclusion the level of plasma GnRHfSH in male rats after microinjection of GnIH into lateral ventricle is lower than that in the control group. The down-regulation of POMC protein expression in hypothalamus. GnIH regulates the hypothalamus-pituitary-gonad axis to some extent, and its mechanism may be related to the inhibition of POMC neuron function.
【作者單位】： 承德醫(yī)學(xué)院;承德醫(yī)學(xué)院附屬醫(yī)院;陸軍總醫(yī)院263臨床部;滄州醫(yī)學(xué)高等�？茖W(xué)校;
【基金】：河北省自然科學(xué)基金資助項(xiàng)目(H2013406115) 河北省科學(xué)技術(shù)研究與發(fā)展計(jì)劃項(xiàng)目(08276101D-20) 河北省高等學(xué)校科學(xué)研究項(xiàng)目(QN2015121) 河北省衛(wèi)生廳醫(yī)學(xué)科學(xué)研究項(xiàng)目(20130021)
【分類號】：R338
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本文編號：1669512