本文選題：電針　切入點(diǎn)：腦出血　出處：《黑龍江中醫(yī)藥大學(xué)》2017年碩士論文

【摘要】：實(shí)驗(yàn)?zāi)康?本實(shí)驗(yàn)意在通過建立動(dòng)物模型,說明電項(xiàng)針對(duì)腦出血后咳嗽反射機(jī)制的重塑作用。實(shí)驗(yàn)方法:取雄性SPF級(jí)豚鼠45只,體重300±15g之間。適應(yīng)性飼養(yǎng)一周后,用枸櫞酸霧化引咳法給予霧化引咳,分別記錄其10分鐘咳嗽次數(shù),將10分鐘內(nèi)咳嗽次數(shù)少于20次或大于100次的豚鼠剔除,剔除過后補(bǔ)充豚鼠至45只(補(bǔ)充豚鼠也要進(jìn)行霧化并不滿足剔除標(biāo)準(zhǔn)),將經(jīng)過篩選的45只豚鼠隨機(jī)分成三組,每組15只,在將每一組隨機(jī)分成三個(gè)小組,每個(gè)小組5只樣本。分組后進(jìn)行造模,方法如下:取雄性SPF級(jí)豚鼠,體重300±15g之間。實(shí)驗(yàn)室適應(yīng)性飼養(yǎng)一周后將待造模樣本用7%水合氯醛(0.5毫升/百克)腹腔注射麻醉,確認(rèn)麻醉劑生效后將待造模樣本以俯臥固定于立體定位儀上,從頭部正中開皮,暴露顱骨,在顱骨正中線左側(cè)5mm,冠狀縫或前鉆一直徑1mm的小孔,立體定向穿刺后,用注射器向左側(cè)基底節(jié)內(nèi)(靶點(diǎn):前囟左側(cè)4-5mm,冠狀縫向前0.2mm,進(jìn)針深度6-7mm,即基底節(jié)區(qū)),將未肝素化的豚鼠自體血0.03ml緩慢推進(jìn)基底節(jié)區(qū),推注后留針片刻,后緩慢出針。術(shù)后局部噴灑慶大霉素,縫合頭皮,局部皮膚采用碘伏消毒。之后空白對(duì)照組不給予任何治療,針刺組給予針刺雙側(cè)風(fēng)池、翳風(fēng)穴,選取針具規(guī)格為0.35×25mm,進(jìn)針深度4mm,針刺過程中行針兩次,每次間隔10分鐘,用平補(bǔ)平瀉手法,每次持續(xù)30秒,留針20分鐘,每日針刺一次。電針組給予同側(cè)風(fēng)池、翳風(fēng)穴連接電針,風(fēng)池穴為正極,翳風(fēng)穴為負(fù)極,輸出波為疏密波,頻率以樣本頭部微顫為標(biāo)準(zhǔn),輸出電流為0.3毫安,留針20分鐘,每天治療一次。后在七天、十四天、二十八天三個(gè)時(shí)間節(jié)點(diǎn)參照LongaFz五級(jí)評(píng)分法評(píng)價(jià)樣本動(dòng)物行為學(xué),記錄樣本10分鐘咳嗽次數(shù)后取樣,按要求測(cè)定樣本血清白細(xì)胞介素4,并在第二十八天取樣本肺組織切片,觀察樣本肺部組織結(jié)構(gòu)變化。實(shí)驗(yàn)結(jié)果:在相同時(shí)間節(jié)點(diǎn)比較樣本10分鐘咳嗽次數(shù),針刺組及電針組與空白對(duì)照組比較差異性顯著(p0.05),具有統(tǒng)計(jì)學(xué)意義。同樣,對(duì)樣本行為學(xué)評(píng)分、血清白細(xì)胞介素等方面針刺組及電針組與空白對(duì)照組比較差異亦性顯著(P0.05),具有統(tǒng)計(jì)學(xué)意義。實(shí)驗(yàn)結(jié)論:1.豚鼠腦出血造模后,咳嗽反射明顯減弱,且隨時(shí)間推移可緩慢恢復(fù)。2.與對(duì)照組比較,針刺與電針均可重塑樣本咳嗽反射。3.分析各項(xiàng)實(shí)驗(yàn)指標(biāo)及組織樣本,說明電針組效果優(yōu)于針刺組。
[Abstract]:Objective: the purpose of this experiment was to establish an animal model to explain the effect of electrical item on the remodeling of cough reflex mechanism after intracerebral hemorrhage. Methods: 45 male SPF guinea pigs weighing between 300 鹵15g were selected and fed adaptively for one week. The cough was induced by atomization of citric acid, and the coughing times of 10 minutes were recorded respectively. The guinea pigs with less than 20 coughs or more in 10 minutes were removed. After culling, the guinea pigs were added to 45 guinea pigs (the addition of aerosols did not meet the elimination criteria. 45 guinea pigs were randomly divided into three groups with 15 guinea pigs in each group, and each group was randomly divided into three groups. Five samples of each group were divided into groups. The following methods were used: male SPF guinea pigs, weighing between 300 鹵15g, were anesthetized by intraperitoneal injection of 7% chloral hydrate (0.5ml / 100g) after a week of adaptive feeding in the laboratory. Confirm that after the anesthetic comes into effect, the shape to be made is fixed on the stereotactic locator with a prone position, the skin is opened from the middle of the head, the skull is exposed, the left side of the midline of the skull is 5mm, the coronal suture or the anterior hole of a diameter 1mm is drilled, and after the stereotactic puncture, Using a syringe to the left basal ganglia (target: anterior fontanelle 4-5mm, coronal suture 0.2mm forward, needle depth 6-7mm, basal ganglia region), the 0.03ml of the unheparinized guinea pig autologous blood was slowly pushed forward to the basal ganglia area, and the needle was kept for a moment. After the operation, gentamicin was sprayed locally, the scalp was sutured, and the local skin was disinfected with iodophor. After that, the blank control group was not given any treatment, and the acupuncture group was given acupuncture at the bilateral Fengchi and Yifeng points. The needle gauge is 0.35 脳 25mm, and the depth of the needle is 4mm. During the acupuncture process, the needle is made twice, with 10 minutes interval, with a flat tonifying and reducing technique, each time lasting 30 seconds, keeping the needle for 20 minutes and needling once a day. The electroacupuncture group is given the same side wind pond and the Yifeng point is connected to the electroacupuncture, The Fengchi point is positive, the Yifeng point is negative, the output wave is a dense wave, the frequency is based on the sample head microtremor, the output current is 0.3 Ma, the needle is kept for 20 minutes and treated once a day. After that, it is treated once a day for seven days and fourteen days. Three time points of 28 days were used to evaluate the animal behavior of the samples according to the LongaFz five-grade scoring method. The samples were sampled after 10 minutes of coughing, and the serum interleukin-4 was measured according to the requirements, and the lung tissue sections were sampled on the 28 day. Observe the changes of lung tissue structure in the sample. Results: at the same time node comparison sample 10 minutes cough times, acupuncture group and electroacupuncture group compared with the blank control group significant difference (p 0.05). The difference between acupuncture group and electroacupuncture group and blank control group was also significant (P 0.05). Conclusion: 1. After making model of guinea pig cerebral hemorrhage, cough reflex was obviously decreased. Compared with the control group, acupuncture and electroacupuncture could reshape the cough reflex of the sample. 3. Analysis of the experimental indexes and tissue samples showed that the effect of the electroacupuncture group was better than that of the acupuncture group.
【學(xué)位授予單位】：黑龍江中醫(yī)藥大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R245

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