本文選題：急性肺損傷　切入點(diǎn)：動物模型　出處：《中國呼吸與危重監(jiān)護(hù)雜志》2017年03期 　論文類型：期刊論文

【摘要】：目的比較氣管內(nèi)滴入脂多糖與腹膜腔注射脂多糖兩種不同方法復(fù)制的急性肺損傷小鼠模型的生理、病理特點(diǎn)及動態(tài)變化。方法 BALB/c小鼠麻醉后分別經(jīng)氣管內(nèi)滴入脂多糖(5 mg/kg)和經(jīng)腹膜腔內(nèi)注射脂多糖(5 mg/kg)。在脂多糖處理后第1、2、6、12、18、24、48 h處死小鼠,通過檢測支氣管肺泡灌洗液(BALF)蛋白含量、肺濕/干重比值和肺組織病理半定量評分評價(jià)呼吸系統(tǒng)損傷程度,ELISA法檢測血清與BALF上清中腫瘤壞死因子-α(TNF-α)濃度以了解全身與肺局部炎癥反應(yīng)程度。結(jié)果兩組脂多糖處理小鼠的BALF中蛋白含量、肺濕/干重比值、血清/BALF中TNF-α濃度均增高。腹膜腔注射脂多糖小鼠肺濕/干重比值較氣管滴入脂多糖小鼠高,其余上述指標(biāo)在兩組間無顯著差異。兩組小鼠在脂多糖處理后肺組織炎癥細(xì)胞浸潤、肺泡間隔增寬、肺組織病理半定量評分均明顯增高,以氣管內(nèi)滴入組病理損傷較顯著。結(jié)論氣管內(nèi)滴入脂多糖與腹腔注射脂多糖均可引起肺局部炎癥與組織損傷,兩種模型病理損傷程度差異較大,自身好轉(zhuǎn)趨勢不同,應(yīng)根據(jù)實(shí)驗(yàn)?zāi)康倪x擇合適的模型復(fù)制方法。
[Abstract]:Objective to compare the physiology of acute lung injury mice induced by intratracheal instillation of lipopolysaccharide and peritoneal injection of lipopolysaccharide. Methods BALB/c mice were treated with lipopolysaccharide (LPS) 5 mg / kg and intraperitoneal lipopolysaccharide 5 mg / kg respectively after anesthesia. The mice were killed at 48 h after lipopolysaccharide treatment. The content of BALF protein in bronchoalveolar lavage fluid (BALF) was measured. Lung wet / dry weight ratio and lung pathology semi-quantitative evaluation of the degree of respiratory injury; Elisa method was used to detect TNF- 偽 TNF- 偽 in serum and BALF supernatant to understand the degree of systemic and regional inflammation. Protein content in BALF of Sugar treated mice, The lung wet / dry weight ratio and TNF- 偽 concentration in serum / BALF were higher in peritoneal injection lipopolysaccharide mice than in trachea instilled lipopolysaccharide mice. There was no significant difference between the two groups in the other indexes mentioned above. After lipopolysaccharide treatment, inflammatory cells infiltrated the lung tissue, the alveolar septum widened, and the semiquantitative score of lung histopathology increased significantly in the two groups. Conclusion both intratracheal lipopolysaccharide and intraperitoneal injection of lipopolysaccharide can cause lung inflammation and tissue injury. The appropriate model replication method should be selected according to the purpose of the experiment.
【作者單位】： 廣州軍區(qū)廣州總醫(yī)院呼吸內(nèi)科;解放軍第458醫(yī)院呼吸內(nèi)科;
【基金】：國家自然科學(xué)基金面上項(xiàng)目(81370173);國家自然科學(xué)基金青年科學(xué)基金(8120002,81200025) 廣東省醫(yī)學(xué)科學(xué)技術(shù)研究基金(A2016305) 廣東省科技計(jì)劃項(xiàng)目(2016ZC0213)
【分類號】：R563.8

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本文編號：1642177