本文選題：關(guān)鍵字　切入點(diǎn)：心臟型脂肪酸結(jié)合蛋白　出處：《皖南醫(yī)學(xué)院》2014年碩士論文　論文類型：學(xué)位論文

【摘要】：目的：構(gòu)建大鼠急性心肌損傷壞死模型的基礎(chǔ)上，檢測(cè)不同時(shí)間點(diǎn)血清中心臟型脂肪酸結(jié)合蛋白(H-FABP)的濃度變化，通過與肌鈣蛋白I（cTnI）對(duì)比，探討H-FABP對(duì)早期診斷急性心肌梗死的價(jià)值。 方法：健康雄性Sprauge Dawley（SD）大鼠（體重220±20克）多點(diǎn)皮下注射異丙腎上腺素10mg/kg,每24小時(shí)注射一次，連續(xù)三天，制作大鼠心肌損傷壞死模型。對(duì)照組給予相同量生理鹽水(10mg/kg）。選取SD大鼠30只，，隨機(jī)分成模型組和對(duì)照組，20只進(jìn)入模型組，10只進(jìn)入對(duì)照組。主要操作步驟如下：術(shù)前大鼠禁食禁水4小時(shí)，稱重后，以1.5%戊巴比妥鈉（3ml/kg）腹腔注射麻醉大鼠后，待肌力減弱時(shí)將大鼠固定在手術(shù)臺(tái)上。打開RM6240多通道生理信號(hào)采集系統(tǒng)，設(shè)定好參數(shù)，將大頭針形插入大鼠四肢皮下，左上肢連接紅色正極，右上肢接綠色負(fù)極，參考極黑色接右下肢，記錄Ⅱ?qū)?lián)心電圖。行氣管插管和頸動(dòng)脈分離后，觀察心電圖基線，待心電圖穩(wěn)定后開始描記心電圖。接著再次予末次多點(diǎn)皮下注射異丙腎上腺素(10mg/kg)，同時(shí)觀察心電圖，記錄不同時(shí)間點(diǎn)心電圖，觀察J點(diǎn)改變?yōu)橹笜?biāo)。并于末次給藥后1小時(shí)、3小時(shí)、6小時(shí)、8小時(shí)經(jīng)頸總動(dòng)脈插管采血，每次約0.5ml，離心后取上清液分裝及做好標(biāo)記后立即送入-80℃冰箱凍存。對(duì)照組給予相同量生理鹽水(10mg/kg），余手術(shù)操作同模型組。均采用酶聯(lián)免疫吸附試驗(yàn)法（ELASA法）分別測(cè)定H-FABP、cTnI濃度，比較H-FABP、cTnI在兩組中的變化。 結(jié)果：1.動(dòng)物存活情況：造模過程中死亡4只，死亡率為13.33%，其中1只死于出血，1只死于氣道阻塞，窒息而死亡，1只死于室顫，1只死于麻醉過量。2.病理學(xué)檢查：模型組心肌病理切片上可見心肌嗜伊紅性較對(duì)照組增強(qiáng)，心肌纖維腫脹變性，肌纖維斷裂，血管擴(kuò)張充血，心肌細(xì)胞可見核碎裂、核溶解，甚至出現(xiàn)點(diǎn)狀、片狀壞死，間質(zhì)中可見大量炎性細(xì)胞浸潤(rùn)。3.心電圖檢查：行心肌損傷模型均可出現(xiàn)心電圖缺血梗死圖形變化，如：J點(diǎn)改變、T波改變，部分Q波形成。4.各組大鼠血清cTnI含量的比較：組內(nèi)不同時(shí)間點(diǎn)比較：對(duì)照組各時(shí)間點(diǎn)差異無統(tǒng)計(jì)學(xué)意義（P＞0.05）；模型組1h與3h組兩者無統(tǒng)計(jì)學(xué)意義（P＞0.05），6h、8h與1h之間差異有統(tǒng)計(jì)學(xué)意義（P＜0.01）；組間不同時(shí)間點(diǎn)比較：除1h、3h外，模型組與對(duì)照組比較差異有統(tǒng)計(jì)學(xué)意義（P＜0.01）。5.各組大鼠血清H-FABP含量的比較：組內(nèi)不同時(shí)間比較：對(duì)照組1h與3h兩組之間差異無統(tǒng)計(jì)學(xué)意義（P＞0.05），6h、8h與1h之間差異有統(tǒng)計(jì)學(xué)意義（P＜0.01）；實(shí)驗(yàn)組各時(shí)間點(diǎn)之間差異有統(tǒng)計(jì)學(xué)意義（P＜0.01）；組間不同時(shí)間點(diǎn)比較：除1h、3h外，模型組與對(duì)照組之間有統(tǒng)計(jì)學(xué)意義（P＜0.01）； 結(jié)論：1.在異丙腎上腺素誘導(dǎo)心肌壞死模型中，肌鈣蛋白在末次給藥后3小時(shí)以內(nèi)尚未升高，H-FABP在3小時(shí)內(nèi)升高，早于肌鈣蛋白。2. H-FABP可作為異丙腎上腺素誘導(dǎo)大鼠心肌壞死早期診斷的指標(biāo)。
[Abstract]:Aim: to study the changes of cardiac fatty acid binding protein (H-FABP) in serum at different time points on the basis of rat model of acute myocardial injury and necrosis, and to compare it with cardiac troponin I ~ (cTnI). To investigate the value of H-FABP in early diagnosis of acute myocardial infarction. Methods: healthy male Sprauge Dawley SD rats (weight 220 鹵20g) were injected with isoproterenol 10mg / kg subcutaneously every 24 hours for three consecutive days. The rat model of myocardial injury and necrosis was made. The control group was given the same amount of normal saline 10 mg / kg. 30 SD rats were selected. The rats were randomly divided into model group (n = 20) and control group (n = 10). The main steps were as follows: the rats were fasting for 4 hours before operation. After weighing, 1.5% pentobarbital sodium was injected intraperitoneally into anesthetized rats. When the muscle strength was weakened, the rats were fixed on the operating table. The RM6240 multi-channel physiological signal acquisition system was turned on, and the parameters were set up. The needle was inserted into the rat limb subcutaneously, the left upper limb was connected with the red positive pole, and the right upper limb was connected with the green negative electrode. The electrocardiogram (ECG) of lead 鈪

本文編號(hào)：1638053