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  本文選題：胃十二指腸動脈　切入點：肝動脈　出處：《貴州醫(yī)科大學》2016年碩士論文　論文類型：學位論文

【摘要】：目的:通過建立一種改良的經(jīng)大鼠胃十二指腸動脈逆行肝動脈插管的新方法,探討其在臨床實驗研究中運用的可行性及價值。方法:選取成年健康清潔級Wistar大鼠120只,隨機平均分為A、B、C、D組,每組30只;其中A組與B組為預實驗組,分別采用改良法與傳統(tǒng)法行肝動脈插管。對于C、D組研究對象,采用穿刺種植法建立大鼠肝癌模型,分別以改良法與傳統(tǒng)法插管行肝動脈造影及介入化學栓塞治療,栓塞材料均選擇碘油,比較兩種方法的插管成功率、大鼠肝癌建模的成功率、腫瘤體積變化、各組大鼠死亡原因及實驗死亡率。結(jié)果:A組插管成功率90.0%(27/30),B組插管成功率63.3%(19/30),A組插管成功率明顯高于B組(?2=4.565,P=0.033)。對C、D組研究對象進行腫瘤種植建立大鼠肝癌模型,C與D兩組間腫瘤種植成功率為100%。待肝癌模型制作成功后,術(shù)前掃描C、D組大鼠肝左葉均可見大小不一的團塊狀低密度影,邊緣較清晰,未見腹水及肝臟子灶形成。經(jīng)過A、B組預實驗后,C組插管成功率93.3%(28/30),D組插管成功率70.0%(21/30),死亡原因可能為血管受損、肝臟損傷及麻醉意外等。C組插管成功率明顯高于D組(?2=4.007,P=0.045)。而C組與D組介入栓塞治療前后平均腫瘤體積無統(tǒng)計學差異(P0.05),但D組大鼠術(shù)后10天死亡率較C組明顯升高(42.86%vs 10.71%,?2=5.079,P=0.024)。結(jié)論:改良經(jīng)胃十二指腸動脈插管造影肝動脈方法簡單、成功率高,術(shù)后死亡率低、并發(fā)癥少;值得繼續(xù)深入研究和的相關實驗中進行嘗試,可進一步加強動物實驗的深度和介入治療基礎研究的廣度,值得繼續(xù)深入研究和的相關實驗中進行嘗試。
[Abstract]:Objective: to establish a new method of retrograde hepatic artery catheterization through gastroduodenal artery in rats and to explore the feasibility and value of its application in clinical experiment. Methods: 120 healthy adult Wistar rats were selected. Group A and group B were randomly divided into two groups: group A and group B were pretreated with hepatic artery catheterization by modified method and traditional method respectively. The liver cancer model of rats was established by puncture and implantation method for the patients in group C and D, which were divided into two groups: group A (n = 30), group B (n = 30), group B (n = 30), group B (n = 30). Hepatic arteriography and interventional chemoembolization were performed by the improved method and the traditional method respectively. Lipiodol was used as the embolization material. The success rate of intubation in the two methods, the success rate of rat liver cancer modeling, and the change of tumor volume were compared. Results the success rate of intubation in group B was significantly higher than that in group B (n = 63.3) and the success rate of intubation in group A was significantly higher than that in group B. The success rate of tumor implantation between group C and group D was 100. When the model of liver cancer was made successfully, the left lobes of liver in group C and D could be seen in the left lobe of rats of different sizes. The success rate of intubation in group C was 93.3%. The success rate of intubation in group D was 70.021 / 30. The cause of death might be vascular injury, liver injury and anaesthesia accident. The success rate of intubation in group C was significantly higher than that in group D. There was no significant difference in mean tumor volume between group C and group D before and after embolization, but the mortality rate of group D was significantly higher than that of group C (42.86 vs 10.71) 10 days after embolization. Conclusion: the improved gastroduodenal artery catheterization is simple, with high success rate, low postoperative mortality and less complications. It can further strengthen the depth of animal experiments and the breadth of basic research of interventional therapy.
【學位授予單位】：貴州醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2016
【分類號】：R735.7

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