本文選題：依托咪酯　切入點(diǎn)：丙泊酚　出處：《桂林醫(yī)學(xué)院》2014年碩士論文　論文類型：學(xué)位論文

【摘要】：目的：觀察并對(duì)比依托咪酯和丙泊酚對(duì)新生大鼠早期空間認(rèn)知能力、探索能力、學(xué)習(xí)記憶能力及海馬組織中神經(jīng)元形態(tài)、Caspase-3、γ-氨基丁酸的影響。方法：將日齡17-18天的SPF級(jí)幼年SD大鼠經(jīng)電迷宮篩選后選取57只（體重31-40g）隨機(jī)平均分為3組，每組19只：對(duì)照組（單次腹腔注射生理鹽水10ml/kg）、依托咪酯組（單次腹腔注射依托咪酯5mg/kg）和丙泊酚組（單次腹腔注射丙泊酚50mg/kg）。對(duì)照組腹腔注射生理鹽水后3小時(shí)及依托咪酯和丙泊酚組麻醉清醒后3h，每組隨機(jī)選取10只行行為學(xué)測(cè)試（曠場(chǎng)實(shí)驗(yàn)、洞板實(shí)驗(yàn)、電迷宮實(shí)驗(yàn)）；每組隨機(jī)選取3只，斷頭法處死，取海馬組織應(yīng)用HE染色法觀察海馬神經(jīng)元的變化和應(yīng)用免疫組化染色法檢測(cè)Caspase-3的表達(dá)情況；每組隨機(jī)選取6只，斷頭法處死，取海馬組織利用全自動(dòng)氨基酸分析儀檢測(cè)GABA的含量。結(jié)果：（1）麻醉前三組大鼠之間的體重比較差異無(wú)統(tǒng)計(jì)學(xué)意義（P＞0.05）；三組大鼠都是經(jīng)過(guò)電迷宮篩選，學(xué)習(xí)記憶能力相比差異無(wú)統(tǒng)計(jì)學(xué)意義（P＞0.05）。（2）曠場(chǎng)實(shí)驗(yàn)測(cè)試結(jié)果比較：依托咪酯和丙泊酚組大鼠在中央格停留的時(shí)間和2min內(nèi)穿越的格子數(shù)（4.40±2.01,4.60±1.96和37.90±11.88,36.30±15.68）與對(duì)照組（3.70±1.06,39.10±11.89）相比（P＞0.05），差異無(wú)統(tǒng)計(jì)學(xué)意義。（3）洞板實(shí)驗(yàn)測(cè)試結(jié)果比較：依托咪酯和丙泊酚組大鼠5min內(nèi)伸入洞的次數(shù)（12.00±3.13,10.00±2.79）與對(duì)照組（16.30±4.62）比較都減少（P＜0.05），，差異有統(tǒng)計(jì)學(xué)意義。（4）電迷宮實(shí)驗(yàn)測(cè)試結(jié)果：①依托咪酯組大鼠正確反應(yīng)次數(shù)和全天總反應(yīng)時(shí)間（12.40±2.07,79.10±11.64）與對(duì)照組（13.30±2.00,67.70±12.18）比較均有改變（P＞0.05），但差異無(wú)統(tǒng)計(jì)學(xué)意；②丙泊酚組大鼠正確反應(yīng)次數(shù)（9.80±2.39）與對(duì)照組比較（P＜0.01）有顯著性差異且與依托咪酯組比較（P＜0.05）差異也有統(tǒng)計(jì)學(xué)意義；丙泊酚組大鼠全天總反應(yīng)時(shí)間（82.30±10.20）與對(duì)照組比較（P＜0.05）差異有統(tǒng)計(jì)學(xué)意義，與依托咪酯組比較（P＞0.05）差異無(wú)統(tǒng)計(jì)學(xué)意義。（5）HE染色結(jié)果比較：三組海馬CA1區(qū)神經(jīng)元數(shù)目和椎體細(xì)胞的形態(tài)無(wú)明顯差異，CA1區(qū)神經(jīng)細(xì)胞形態(tài)正常，排列基本規(guī)則，神經(jīng)元密度均勻，未見明顯椎體細(xì)胞變性、壞死。（6）Caspase-3免疫組化染色結(jié)果比較：海馬CA1區(qū)神經(jīng)元Caspase-3表達(dá)情況，胞漿中出現(xiàn)棕黃色顆粒為陽(yáng)性細(xì)胞（顯微鏡倍數(shù)×400，高倍鏡視野下每個(gè)標(biāo)本觀察10個(gè)視野，取總和）。依托咪酯和丙泊酚組海馬神經(jīng)元Caspase-3表達(dá)的細(xì)胞計(jì)數(shù)分別為（35.67±1.53，37.33±2.31）均高于C組（32.33±2.52），但差異無(wú)統(tǒng)計(jì)學(xué)意義（P＞0.05）。（7）依托咪酯和丙泊酚組海馬組織γ-氨基丁酸含量（47.88±10.86，73.14±11.79）都高于對(duì)照組（39.08±10.56），但依托咪酯組與對(duì)照組比較（P＞0.05）差異無(wú)統(tǒng)計(jì)學(xué)意義，丙泊酚組與對(duì)照組比較（P＜0.01）有極顯著性差異；丙泊酚組與依托咪酯組比較（P＜0.01）也有極顯著性差異。結(jié)論： （1）單次腹腔注射5mg/kg依托咪酯和50mg/kg丙泊酚對(duì)新生大鼠早期認(rèn)知功能均有影響，依托咪酯主要表現(xiàn)在探索能力，丙泊酚現(xiàn)在探索能力和學(xué)習(xí)記憶能力兩方面。（2）依托咪酯和丙泊酚對(duì)海馬組織神經(jīng)元形態(tài)、Caspase-3的表達(dá)均無(wú)明顯影響。（3）丙泊酚增加γ-氨基丁酸的合成和分泌，依托咪酯對(duì)γ-氨基丁酸的合成和分泌無(wú)明顯影響，可能是造成兩種藥物對(duì)新生大鼠早期的認(rèn)知功能影響差異的原因。
[Abstract]:Objective: To observe and contrast of etomidate and propofol on early exploration ability of spatial cognition, neonatal rat, neuronal morphology, the ability of learning and memory in the hippocampus of Caspase-3. The effect of gamma aminobutyric acid. Methods: 17-18 day old SPF young SD rats by electric maze after screening a total of 57 (weight 31-40g) were randomly divided into 3 groups, 19 rats in each group: control group (single intraperitoneal injection of saline 10ml/kg), etomidate group (single intraperitoneal injection of etomidate and propofol group (5mg/kg) by single intraperitoneal injection of propofol 50mg/kg). The control group 3 hours after intraperitoneal injection of saline and etomidate and propofol group after anesthesia 3h, 10 rats in each group were randomly selected for behavioral test (open field test, hole board test and electric maze experiment); each group of 3 randomly selected rats were killed by decapitation, hippocampus tissues were observed with HE staining of the hippocampal neurons Changes and immunohistochemical staining was used to detect the expression of Caspase-3 in each group were randomly selected; 6 rats were killed by decapitation, hippocampus content by automatic amino acid analyzer for detection of GABA. Results: (1) the difference between the three groups before anesthesia, the body weight of rats was not statistically significant (P > 0.05); three rats are after maze screening, the ability of learning and memory had no significant difference (P > 0.05). (2) compared with the open field test results: etomidate and propofol group rats stay in the central square of time and 2min through the lattice number (4.40 + 2.01,4.60 + 1.96 and 37.90 + 11.88,36.30 + 15.68) and control group (3.70 + 1.06,39.10 + 11.89) compared (P > 0.05), the difference was not statistically significant. (3) comparison of testing results in experimental hole: number of etomidate and propofol in rats of group 5min and extending into the hole (12 + 3.13,10.00 + 2.79) and the control Group (16.30 + 4.62) were decreased (P < 0.05), the difference was statistically significant. (4) test results of electric maze experiment: the correct response times and etomidate group rats all day long the total reaction time (12.40 + 2.07,79.10 + 11.64) and control group (13.30 + 2.00,67.70 + 12.18) is changed significantly (P > 0.05), but no statistical difference; the number of correct responses in the propofol group rats (9.80 + 2.39) compared with the control group (P < 0.01) and there was significant difference compared with etomidate group (P < 0.05) there were significant differences; Propofol group rats all day long the total reaction time (82.30. 10.20) compared with the control group (P < 0.05) the difference was statistically significant, compared with etomidate group (P > 0.05) showed no significant difference. (5) the results of HE staining: comparison of the three groups the number of hippocampal CA1 neurons and pyramidal cells form no significant difference, the morphology of CA1 neurons in normal, row Among the basic rules, the neuron density uniform, no obvious vertebral cell degeneration and necrosis. (6) Caspase-3 immunohistochemical staining results: the expression of Caspase-3 in hippocampus CA1 neurons, the cytoplasm appeared Brown particles as positive cells (400 x magnification, at higher magnification of each sample in 10 visual fields, take total). The expression of etomidate and propofol group Caspase-3 in hippocampus cell count respectively (35.67 + 1.53,37.33 + 2.31) were higher than that of group C (32.33 + 2.52), but the difference was not statistically significant (P > 0.05). (7) etomidate and propofol group hippocampus GABA content (47.88 + 10.86,73.14 + 11.79) are higher than that of the control group (39.08 + 10.56), but etomidate group compared with the control group (P > 0.05) there was no significant difference compared with the control group, propofol group (P < 0.01) has significant differences; Propofol group and etomidate group The comparison (P < 0.01) also had very significant difference.
(1) a single intraperitoneal injection of etomidate and 50mg/kg 5mg/kg effects of propofol on early cognitive function in neonatal rats were mainly manifested in the ability to explore, etomidate, propofol now exploration ability and the ability of learning and memory in two aspects. (2) of etomidate and propofol on hippocampal neuron morphology in Textile Group, had no effect on the expression of Caspase-3 (3). Increased synthesis of GABA and secretion of propofol, no obvious effect of etomidate on GABA synthesis and secretion, may be the cause of the effect of two kinds of drugs on cognitive function in early neonatal rats difference.

【學(xué)位授予單位】：桂林醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R614.2

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