發(fā)布時(shí)間：2018-03-08 11:40

  本文選題：骨密度　切入點(diǎn)：骨組織形態(tài)　出處：《湖南中醫(yī)藥大學(xué)》2014年碩士論文　論文類型：學(xué)位論文

【摘要】：目的：通過(guò)檢測(cè)虎潛丸干預(yù)對(duì)去卵巢大鼠骨密度及骨組織中BMP-7水平的影響,探討虎潛丸防治骨質(zhì)疏松癥的療效。 方法：將6個(gè)月齡的40只雌性SD大鼠隨機(jī)分為手術(shù)模型組、雌激素組、虎潛丸組和假手術(shù)組,每組10只。采用10%水合氯醛溶液對(duì)SD大鼠按0.33mL/100g體質(zhì)腹腔注射進(jìn)行麻醉,麻醉生效后,在無(wú)菌條件下,前三組由背側(cè)入路,肋弓下緣備皮,絡(luò)合碘消毒,切開皮膚,在脊柱旁開0.5cm與肋弓下緣0.5cm的交點(diǎn)處,進(jìn)入腹腔剪去卵巢。另側(cè)同法切除。假手術(shù)組大鼠也行同樣手術(shù)入路打開腹腔,只切除腹膜后少許脂肪組織,不切除卵巢。切口內(nèi)滴入少量青霉素水溶液,分層縫合手術(shù)切口。術(shù)后分籠飼養(yǎng),用5萬(wàn)U單位青霉素注射液腹腔注射3d抗感染。術(shù)后4周,從造模組及假手術(shù)組中隨機(jī)各選出6只大鼠行活體骨密度檢查,以確定造模成功。在確定造模成功后,虎潛丸組給予虎潛丸煎劑濃縮液灌胃,每天1次；雌激素組給予尼爾雌醇懸濁液灌胃,每周1次；手術(shù)模型組和假手術(shù)組給予蒸餾水灌胃,每天1次。灌胃治療12周后測(cè)量各組大鼠的骨密度及骨組織中BMP-7含量。 結(jié)果：手術(shù)模型組大鼠的骨密度低于假手術(shù)組,其差異具有統(tǒng)計(jì)學(xué)意義(P0.05),說(shuō)明骨質(zhì)疏松大鼠模型已建立；虎潛丸組及雌激素組大鼠骨密度均高于手術(shù)模型組,其差異具有統(tǒng)計(jì)學(xué)意義(P0.05),說(shuō)明兩種治療方式均有治療效果；虎潛丸組與雌激素組大鼠骨密度相比較無(wú)明顯差距,無(wú)統(tǒng)計(jì)學(xué)意義(P0.05)。BMP-7陽(yáng)性表達(dá)方面,虎潛丸組及雌激素組大鼠骨組織中BMP-7水平均高于手術(shù)模型組,其差異具有統(tǒng)計(jì)學(xué)意義(P0.05),虎潛丸組與雌激素組大鼠BMP-7水平相比較無(wú)明顯差距,無(wú)統(tǒng)計(jì)學(xué)意義(P0.05) 結(jié)論：虎潛丸對(duì)切除卵巢后所致的骨質(zhì)疏松癥有明顯的治療作用,其機(jī)制可能是在一定程度地提高了組織中BMP-7的水平,從而抑制骨組織的吸收,促進(jìn)骨組織的形成。
[Abstract]:Objective: to investigate the effect of Huqian pill on bone mineral density (BMD) and BMP-7 level in bone tissue of ovariectomized rats. Methods: forty female Sprague-Dawley rats aged 6 months were randomly divided into three groups: estrogen group, Huqian pill group and sham-operation group, 10 rats in each group. 10% chloral hydrate solution was used to anesthetize SD rats by intraperitoneal injection of 0.33 mL / 100 g constitution. After the anaesthesia took effect, under sterile conditions, the first three groups were treated by dorsal approach, the lower margin of the costal arch was covered with skin, the skin was disinfected with iodine, the skin was cut open, and the intersection between 0.5 cm of the spine and 0.5 cm of the lower margin of the rib arch was opened at the side of the spine. The rats in the sham-operation group also underwent the same operation to open the abdominal cavity, only a little fat tissue was removed from the peritoneum, and the ovary was not removed. A small amount of penicillin solution was dripped into the incision. After operation, 50,000 U penicillin injection was injected intraperitoneally for 3 days to resist infection. At 4 weeks after operation, 6 rats were randomly selected from the model group and sham-operation group to perform bone mineral density examination in vivo. In order to confirm the success of model making, the group of Huqian pills was given concentrated decoction of Huqian pills once a day, the estrogen group was given Neilestriol suspension once a week. Rats in the operation model group and the sham operation group were given distilled water once a day. The bone mineral density (BMD) and the content of BMP-7 in bone tissue were measured after 12 weeks of gastric perfusion. Results: the BMD of the rats in the operation model group was lower than that in the sham operation group, and the difference was statistically significant (P 0.05), which indicated that the osteoporosis rat model had been established, the bone mineral density of the rats in the Huqian pill group and the estrogen group were higher than that in the operation model group. There was no significant difference in bone mineral density (BMD) between the two groups, and there was no significant difference in BMD between the two groups, and there was no significant difference in the positive expression of P0.05U. BMP-7 between the two groups, and there was no significant difference in bone mineral density between the two groups, and there was no significant difference in the expression of BMD between the two groups. The level of BMP-7 in the bone tissue of rats in the Huqian pill group and estrogen group was higher than that in the operation model group, and the difference was statistically significant (P 0.05). There was no significant difference in the level of BMP-7 between the Huqian pill group and the estrogen group (P 0.05). Conclusion: Huqian pill has obvious therapeutic effect on osteoporosis caused by ovariectomy, and its mechanism may be to increase the level of BMP-7 in tissues to a certain extent, thus inhibit the absorption of bone tissue and promote the formation of bone tissue.
【學(xué)位授予單位】：湖南中醫(yī)藥大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R285.5

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