本文選題：臭氧化鹽水　切入點：大鼠切口痛　出處：《中南大學》2014年碩士論文　論文類型：學位論文

【摘要】：摘要 目的：觀察不同濃度臭氧化鹽水靜脈注射對大鼠切口痛模型行為學及血漿TNF-α、IL-6水平的影響,探討靜脈應用臭氧化鹽水對大鼠急性疼痛的鎮(zhèn)痛效果、合理治療濃度、不良反應及其可能機制。 方法：健康成年雄性SD大鼠54只,隨機選取24只用于疼痛行為學測試,30只用于酶聯(lián)免疫吸附(ELISA)法檢測大鼠血漿TNF-α和IL-6濃度。其中,用于疼痛行為學測試的24只大鼠隨機分為4組,每組6只,A、B、C、D組分別于造模前10min經(jīng)尾靜脈給藥,A組給氧氣化鹽水,B、C、D組分別給濃度20μg/m1.3μg/m1.40μg/ml的臭氧化鹽水,四組均給藥5ml/kg,七氟烷麻醉后按Brennan法制作切口痛模型,分別在術前24h、術后2、6、24、48h五個時間點測試并記錄大鼠50%機械縮足閾值和累積疼痛評分。用于血漿TNF-α和IL-6濃度檢測的30只大鼠隨機分為5組,每組6只,A、B、C、D組處理同前,E組為假手術組,吸入七氟烷之后不做處理,術后2h用10%水合氯醛腹腔麻醉后,快速打開胸腔,右心室取血2ml,用ELISA法檢測大鼠血漿TNF-α和IL-6水平。同時,觀察記錄每只靜脈注射臭氧化鹽水的大鼠的不良反應。 結果：(1)不同濃度臭氧化鹽水靜脈注射后,均未發(fā)現(xiàn)大鼠血管炎、死亡的不良事件。 (2)術前24h各組大鼠50%PWMT(g)比較無明顯差別(P0.05),術后2h、6h、48h各組大鼠50%PWMT有顯著差異(P0.05),A組與B組比較在各時間點50%PWMT差異均無統(tǒng)計學意義(P0.05),而A組與C、D兩組比較在術后2、6、24、48h50%PWMT明顯減小,差異均有統(tǒng)計學意義(P0.05),C組與D組在各時間點50%PWMT差異均無統(tǒng)計學意義(P0.05)。 (3)術前24h各組大鼠CPS均為0,術后各時間點各組大鼠CPS均有顯著差異(P0.01),且差異隨時間逐漸變小。A組在術后不同時點CPS分別較B、C、D組高,均有顯著差異(P0.01),且·D組與A組差異最大,C組次之。 (4)術后2h各組大鼠血漿TNF-α濃度有顯著差異(P0.001)。兩兩比較,E組與B、D兩組差異無統(tǒng)計學意義(P0.05),A組和C組較E組血漿TNF-α濃度均升高,其中C組升高較A組明顯。 (5)術后2h各組大鼠血漿IL-6濃度有顯著差異(P0.001)。兩兩比較,E組與A組差異無統(tǒng)計學意義(P0.05),B、C、D組較E組血漿IL-6濃度均升高,其中D組最高,B組最低。 結論：(1)20-40μg/ml的臭氧化鹽水靜脈注射對大鼠切口痛有鎮(zhèn)痛效果。 (2)其鎮(zhèn)痛效果與臭氧化鹽水濃度存在劑量依賴性,濃度越高鎮(zhèn)痛效果越強。 (3)30μg/ml的臭氧化鹽水靜脈注射會使大鼠血漿中TNF-α水平升高,20-40μg/ml的均使血漿IL-6水平升高。 (4)20～40μg/ml的臭氧化鹽水靜脈注射對大鼠無明顯不良反應。
[Abstract]:Abstract. Objective: to observe the effect of different concentrations of ozonated saline on the behavior of rat incision pain model and the level of plasma TNF- 偽 IL-6, and to explore the analgesic effect and reasonable therapeutic concentration of ozonated saline on acute pain in rats. Adverse reactions and their possible mechanisms. Methods: Twenty-four male Sprague-Dawley (SD) rats were randomly selected to measure plasma TNF- 偽 and IL-6 by enzyme linked immunosorbent assay (Elisa). Twenty-four rats were randomly divided into 4 groups. Six rats in each group were given oxygen vaporized brine (OGSCC) (20 渭 g / m 1.3 渭 g 路m ~ (1.40) 渭 g / ml) in group A (n = 10), respectively. All the four groups were given 5 ml / kg sevoflurane to make incision pain model according to Brennan method. The threshold of mechanical contraction and the cumulative pain score of 50% rats were measured and recorded at 24 hours before operation and 24 hours after operation. 30 rats who were used to detect plasma TNF- 偽 and IL-6 concentration were randomly divided into 5 groups. After inhaling sevoflurane, the rats were anesthetized intraperitoneally with 10% chloral hydrate at 2 hours after operation. The thoracic cavity was quickly opened and blood was taken from the right ventricle at 2 ml. The plasma levels of TNF- 偽 and IL-6 were measured by ELISA method. At the same time, Adverse reactions were recorded in each rat intravenously injected with ozonated saline. Results (1) there were no adverse events of vasculitis and death in rats after intravenous injection of different concentrations of ozonated saline. (2) there was no significant difference in 50 PWMTg between the two groups 24 hours before operation (P 0.05), but there was no significant difference in 50 PWMT between group A and group B at 2 h to 48 h after operation. There was no significant difference in 50 PWMT between group A and group B at each time point, but the PWMT of group A and group C decreased significantly at 2 hours after operation, 24 minutes and 48 hours after operation, compared with group B, there was no significant difference between group A and group C in terms of PWMT (P 0.05), and there was no significant difference between group A and group C (P 0.05), and there was no significant difference between group A and group C (P 0.05). There was no significant difference in PWMT between group C and group D at each time point. (3) the CPS of each group was 0 24 hours before operation. There was significant difference in CPS of each group at each time point after operation. The difference gradually decreased with time. The CPS of group A was higher than that of group D at different time points after operation. There was significant difference between group D and group A (P 0.01), and group 路D had the highest difference between group A and group C. (4) there was a significant difference in plasma TNF- 偽 concentration between two groups at 2 h after operation (P 0.001). There was no significant difference between group E and group B (P 0.05) and group C (P 0.05) and group C (P < 0.05). The plasma TNF- 偽 level in group C was significantly higher than that in group A (P < 0.05), and that in group C was significantly higher than that in group A (P < 0.05). (5) there was significant difference in plasma IL-6 concentration between two groups at 2 h after operation (P 0.001). There was no significant difference between E group and A group. The plasma IL-6 concentration in group D was higher than that in group E, and the highest in group D was the lowest in group B. Conclusion 20-40 渭 g / ml of 20-40 渭 g / ml of ozonated saline has analgesic effect on incision pain in rats. (2) the analgesic effect is dose-dependent with the concentration of ozonated saline, and the higher the concentration is, the stronger the analgesic effect is. 30 渭 g / ml ozonated saline intravenously increased the level of TNF- 偽 in plasma of rats, and 20-40 渭 g / ml of TNF- 偽 increased the level of IL-6 in plasma of rats. 40 渭 g / ml of 40 渭 g / ml ozonated saline had no significant adverse effects on rats.
【學位授予單位】：中南大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R614

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