本文選題：芍藥苷　切入點(diǎn)：心肌重構(gòu)　出處：《中國(guó)藥理學(xué)與毒理學(xué)雜志》2017年05期 　論文類型：期刊論文

【摘要】：目的通研究芍藥苷(PEF)對(duì)異丙腎上腺素(ISO)誘導(dǎo)大鼠心肌重構(gòu)的干預(yù)作用,并探討可能的作用機(jī)制。方法雄性SD大鼠32只,隨機(jī)分成4組,即對(duì)照組、模型組、芍藥苷低劑量組、高劑量組(40,80 mg·kg~(-1)),模型組和高、低劑量組連續(xù)皮下注射ISO(5 mg·kg~(-1))1周,對(duì)照組注射等比例生理鹽水;造模同日起,高、低劑量組灌胃芍藥苷,對(duì)照組和模型組灌胃等體積的生理鹽水,連續(xù)14 d。末次給藥后30 min,水合氯醛麻醉大鼠,腹主動(dòng)脈取血測(cè)定羥脯氨酸;處死大鼠,取出心臟分離左心室并稱重,測(cè)定左心室指數(shù);Masson染色觀察組織中心肌纖維化程度;心肌勻漿測(cè)定組織超氧化物歧化酶(SOD)、丙二醛(MDA)及Ⅰ型膠原蛋白的含量。結(jié)果模型組血清羥脯氨酸、左心室指數(shù)及心組織MDA、Ⅰ型膠原蛋白含量均高于對(duì)照組(P0.05),芍藥苷給藥組均低于模型組(P0.05)。在Masson染色中,模型組心肌組織中的膠原明顯增加;芍藥苷低、高劑量組心肌組織中膠原纖維減少,不同劑量心肌組織膠原纖維減少不同,表現(xiàn)出對(duì)ISO引起大鼠心肌纖維化的干預(yù)作用。模型組大鼠組織SOD的活力降低;而芍藥苷高、低劑量均可明顯提高SOD活性模型組。結(jié)論芍藥苷對(duì)ISO致大鼠心肌重構(gòu)具有一定的干預(yù)作用,其作用機(jī)制可能與抗氧化作用有關(guān)。
[Abstract]:Objective to study the effect of paeoniflorin (PEF) on myocardial remodeling induced by isoproterenol (ISO) in rats. Methods 32 male SD rats were randomly divided into 4 groups: control group, model group and low dose group. ISO(5 mg 路kg~(-1))1 was injected subcutaneously into the model group and high dose group, the control group was injected with the same proportion of normal saline, and the high and low dose groups were given paeoniflorin by stomach from the same day, and the control group and the model group were given the same volume of normal saline. The rats were anesthetized with chloral hydrate for determination of hydroxyproline in abdominal aorta 30 min after the last administration, the left ventricle was taken out and weighed, and the degree of myocardial fibrosis was observed by Masson staining. The contents of superoxide dismutase (SOD), malondialdehyde (MDA) and collagen 鈪，

本文編號(hào)：1573041