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  本文關鍵詞： 大鼠模型 病態(tài)竇房結綜合征 心率 細辛　出處：《黑龍江中醫(yī)藥大學》2014年碩士論文　論文類型：學位論文

【摘要】：目的：(1)為研制出一種簡單精確損傷小的病態(tài)竇房結綜合征大鼠模型,進一步改進造模方法,簡化實驗操作,提高造模成功率；(2)研究細辛對病態(tài)竇房結綜合征模型心率的影響；(3)探討細辛作用于病態(tài)竇房結綜合癥大鼠模型的機制,分析其在病竇綜合征中對心率影響的機理。 方法：(1)使用心電篩查健康大鼠,擇40只HR在350-450次/分的大鼠。乙醚麻醉后,于大鼠胸骨右緣第二肋間處皮膚剪一約1毫米小口,并于開口周圍作預荷包縫合穿線。將自制標測電極導管經(jīng)由該口緊貼胸骨右緣第2肋間插入,給藥部位根據(jù)心電圖波形來確定,使用微量注射器經(jīng)自制標測電極導管緩緩推注氫氧化鈉,進行局部浸潤以建立sSS大鼠模型,最后進行完整的荷包縫合。(2)從40只大鼠病態(tài)竇房結綜合征模型中,選取32只成功模型大鼠并隨機分組：生理鹽水組、細辛低劑量組、細辛中劑量組、細辛高劑量組。分別在給藥前、藥物實驗開始后3d、1w、2w、3w測量心率,并對比各組療效。 結果：(1)氫氧化鈉介入法制作的大鼠模型的病生理改變均符合病竇標準,且縮短了造模時間及減小了損傷；(2)細辛各劑量組心率改善情況如下：細辛高劑量組細辛中劑量組細辛低劑量組生理鹽水組。 結論：(1)改進后的氫氧化鈉介入法制作大鼠病態(tài)竇房結綜合癥模型方法簡化了以往的造模方法,提高了模型的穩(wěn)定性,降低了對模型的損傷程度,使建立模型的成功率有了進一步的提高；(2)細辛對改善大鼠病態(tài)竇房結綜合癥心率有一定療效。
[Abstract]:Objective to develop a simple and accurate rat model of sick sinus syndrome (SSS), to further improve the method of modeling, simplify the experimental operation and increase the success rate of modeling. (2) to study the effect of Asarum on heart rate in sick sinus syndrome model. (3) to explore the mechanism of Asarum on the rat model of sick sinus node syndrome (SSS), and to analyze the mechanism of its effect on heart rate in sick sinus syndrome (SSS). Methods the healthy rats were screened by electrocardiogram, and 40 rats with HR of 350-450 beats / min were selected. After anesthesia with ether, the skin of the second intercostal region of the sternum of the rats was cut off about 1 mm. The self-made measuring electrode catheter was inserted through the second intercostal of the right margin of sternum close to the orifice, and the place of administration was determined according to the waveform of electrocardiogram (ECG). The rat model of sSS was established by injecting sodium hydroxide into the microinjector through self-made measuring electrode catheter. Finally, complete purse suture. (2) from 40 rat models of sick sinus syndrome, 32 successful model rats were selected and randomly divided into normal saline group, low dose group and middle dose group. The heart rate was measured 3 days before administration and 3 days after the start of the drug experiment in the high dose group. The heart rate was measured and the curative effect was compared in each group. Results (1) the pathological changes of the rat model made by sodium hydroxide interventional method were in accordance with the standard of sick sinus, and the time of modeling was shortened and the injury was reduced. (2) the heart rate of each dose group was improved as follows: high dose group, medium dose group, low dose group, normal saline group. Conclusion (1) the modified sodium hydroxide interventional method simplifies the previous modeling method, improves the stability of the model and reduces the degree of damage to the model. The success rate of modeling has been further improved; 2) Asarum has a certain effect on improving the heart rate of sick sinus node syndrome in rats.
【學位授予單位】：黑龍江中醫(yī)藥大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R285.5

【參考文獻】

相關期刊論文 前10條

1 于蘇國，孫吉花，，王健本;大鼠心臟竇房結觀察[J];濱州醫(yī)學院學報;1996年06期

2 張浩;徐志云;段煉;宋智鋼;鄒毅清;宗剛軍;;自體竇房結細胞移植治療心臟傳導阻滯的初步研究[J];第二軍醫(yī)大學學報;2007年03期

3 張志輝;程軍;宋治遠;;竇房結區(qū)甲醛濕敷法建立犬竇房結損傷模型[J];第三軍醫(yī)大學學報;2009年22期

4 陳永春;;辨證治療病態(tài)竇房結綜合征30例[J];光明中醫(yī);2011年02期

5 張全文;謝忠禮;;復方丹參滴丸治療竇性心動過緩23例[J];國醫(yī)論壇;2007年04期

6 張倩;馮艷敏;張軍;趙衛(wèi);;升陷湯加味方對兔病態(tài)竇房結綜合征模型心率及竇房結電生理、組織病理的影響[J];河北中醫(yī);2011年05期

7 余廷佑;;環(huán)磷酸腺苷葡胺治療病態(tài)竇房結綜合征臨床應用研究[J];醫(yī)藥論壇雜志;2009年06期

8 駱峰,王東明,周敬群,李玉光,朱平;竇房結功能障礙時心房電活動的變化[J];臨床心血管病雜志;2003年12期

9 王慶志,劉恒興,蔡全云,郭志坤;缺血預處理對缺血再灌注竇房結細胞凋亡及bcl-2和bax基因表達的影響[J];臨床心血管病雜志;2005年05期

10 宋業(yè)琳;孫蘭軍;趙英強;杜桂琴;李杰;;起搏膠囊治療家兔病態(tài)竇房結綜合征的實驗研究[J];臨床心血管病雜志;2007年06期

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