本文關(guān)鍵詞： 大鼠模型 病態(tài)竇房結(jié)綜合征 心率 細(xì)辛　出處：《黑龍江中醫(yī)藥大學(xué)》2014年碩士論文　論文類型：學(xué)位論文

【摘要】：目的：(1)為研制出一種簡單精確損傷小的病態(tài)竇房結(jié)綜合征大鼠模型,進(jìn)一步改進(jìn)造模方法,簡化實(shí)驗(yàn)操作,提高造模成功率；(2)研究細(xì)辛對(duì)病態(tài)竇房結(jié)綜合征模型心率的影響；(3)探討細(xì)辛作用于病態(tài)竇房結(jié)綜合癥大鼠模型的機(jī)制,分析其在病竇綜合征中對(duì)心率影響的機(jī)理。 方法：(1)使用心電篩查健康大鼠,擇40只HR在350-450次/分的大鼠。乙醚麻醉后,于大鼠胸骨右緣第二肋間處皮膚剪一約1毫米小口,并于開口周圍作預(yù)荷包縫合穿線。將自制標(biāo)測(cè)電極導(dǎo)管經(jīng)由該口緊貼胸骨右緣第2肋間插入,給藥部位根據(jù)心電圖波形來確定,使用微量注射器經(jīng)自制標(biāo)測(cè)電極導(dǎo)管緩緩?fù)谱溲趸c,進(jìn)行局部浸潤以建立sSS大鼠模型,最后進(jìn)行完整的荷包縫合。(2)從40只大鼠病態(tài)竇房結(jié)綜合征模型中,選取32只成功模型大鼠并隨機(jī)分組：生理鹽水組、細(xì)辛低劑量組、細(xì)辛中劑量組、細(xì)辛高劑量組。分別在給藥前、藥物實(shí)驗(yàn)開始后3d、1w、2w、3w測(cè)量心率,并對(duì)比各組療效。 結(jié)果：(1)氫氧化鈉介入法制作的大鼠模型的病生理改變均符合病竇標(biāo)準(zhǔn),且縮短了造模時(shí)間及減小了損傷；(2)細(xì)辛各劑量組心率改善情況如下：細(xì)辛高劑量組細(xì)辛中劑量組細(xì)辛低劑量組生理鹽水組。 結(jié)論：(1)改進(jìn)后的氫氧化鈉介入法制作大鼠病態(tài)竇房結(jié)綜合癥模型方法簡化了以往的造模方法,提高了模型的穩(wěn)定性,降低了對(duì)模型的損傷程度,使建立模型的成功率有了進(jìn)一步的提高；(2)細(xì)辛對(duì)改善大鼠病態(tài)竇房結(jié)綜合癥心率有一定療效。
[Abstract]:Objective to develop a simple and accurate rat model of sick sinus syndrome (SSS), to further improve the method of modeling, simplify the experimental operation and increase the success rate of modeling. (2) to study the effect of Asarum on heart rate in sick sinus syndrome model. (3) to explore the mechanism of Asarum on the rat model of sick sinus node syndrome (SSS), and to analyze the mechanism of its effect on heart rate in sick sinus syndrome (SSS). Methods the healthy rats were screened by electrocardiogram, and 40 rats with HR of 350-450 beats / min were selected. After anesthesia with ether, the skin of the second intercostal region of the sternum of the rats was cut off about 1 mm. The self-made measuring electrode catheter was inserted through the second intercostal of the right margin of sternum close to the orifice, and the place of administration was determined according to the waveform of electrocardiogram (ECG). The rat model of sSS was established by injecting sodium hydroxide into the microinjector through self-made measuring electrode catheter. Finally, complete purse suture. (2) from 40 rat models of sick sinus syndrome, 32 successful model rats were selected and randomly divided into normal saline group, low dose group and middle dose group. The heart rate was measured 3 days before administration and 3 days after the start of the drug experiment in the high dose group. The heart rate was measured and the curative effect was compared in each group. Results (1) the pathological changes of the rat model made by sodium hydroxide interventional method were in accordance with the standard of sick sinus, and the time of modeling was shortened and the injury was reduced. (2) the heart rate of each dose group was improved as follows: high dose group, medium dose group, low dose group, normal saline group. Conclusion (1) the modified sodium hydroxide interventional method simplifies the previous modeling method, improves the stability of the model and reduces the degree of damage to the model. The success rate of modeling has been further improved; 2) Asarum has a certain effect on improving the heart rate of sick sinus node syndrome in rats.
【學(xué)位授予單位】：黑龍江中醫(yī)藥大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R285.5

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