本文關(guān)鍵詞：UL對清醒大鼠MVN區(qū)Glu含量和c-Fos蛋白表達的影響　出處：《延邊大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 前庭代償 前庭內(nèi)側(cè)核 谷氨酸 c-Fos蛋白 清醒

【摘要】：前庭器官,可以感受空間位置和運動狀態(tài)。末梢前庭感受器將軀體的運動和頭部空間位置轉(zhuǎn)變?yōu)閭魅胄盘?經(jīng)前庭神經(jīng)纖維通路傳至前庭神經(jīng)核(vestibular nucleus,VN)。VN接受外周前庭器官的神經(jīng)信號,并與視覺和本體感覺共同維持機體平衡狀態(tài)。單側(cè)前庭迷路破壞術(shù)(unilaterallabyrinthectomy,UL)后,前庭代償是研究病變后中樞神經(jīng)系統(tǒng)可塑性的理想實驗?zāi)Ｐ�。UL后,可出現(xiàn)頭偏位、眼震顫和水平旋轉(zhuǎn)等體征。但是前庭功能失衡體征和癥狀會隨著時間的推移逐漸消失,稱為前庭代償(vestibularcompensation,VC)。韓國的樸炳林等人在破壞外周前庭器官的麻醉大鼠觀察到,前庭代償急性期雙側(cè)前庭神經(jīng)內(nèi)側(cè)核區(qū)c-Fos不對稱表達,大約6 d這種不對稱表達消失。本實驗室發(fā)現(xiàn),麻醉大鼠單側(cè)耳內(nèi)灌注2%利多卡因之后,短時阻斷了前庭信號的傳入,前庭內(nèi)側(cè)核(medial vestibular neuclei,MVN)谷氨酸(glutamate,Glu)的含量出現(xiàn)不對稱表達,說明Glu可能與前庭代償有關(guān)聯(lián)。但是,到目前為止這些實驗都是在麻醉動物進行的,尚無前庭代償不同時期MVN區(qū)谷氨酸含量變化的系統(tǒng)研究報道,更無在清醒狀態(tài)下的相關(guān)研究。本研究擬在清醒動物利用核團微量透析、高效液相分析、免疫組織化學(xué)和蛋白印跡法,觀察單側(cè)破壞外周前庭器官不同時期清醒大鼠MVN區(qū)Glu含量和c-Fos蛋白動態(tài)變化,探討前庭代償過程中是有Glu及其NMDA受體的參與。實驗結(jié)果如下:1.UL后,6h頭偏向損傷側(cè),并在24h開始加重,72h達到高峰并不緩解。眼震顫在破壞24 h達到最高峰,48 h迅速緩解,破壞120 h消失。水平旋轉(zhuǎn)在破壞6h出現(xiàn)12h后減弱,96h達到高峰,168 h消失。2.UL后,MVN區(qū)細胞外液中Glu的含量:損傷同側(cè)24、12 h達高峰,144 h基本恢復(fù);損傷對側(cè)6 h達高峰,12小時下降,48 h接著增高,72 h達第二次高峰,144 h基本恢復(fù)。MVN區(qū)雙側(cè)在6、12、24、72、96 h、存在著顯著差異(p0.05,n=12)。3.UL后,MVN區(qū)中c-Fos免疫陽性神經(jīng)元的數(shù)量:損傷同側(cè)24,12 h達高峰,96 h基本恢復(fù);損傷對側(cè)6 h達高峰,12小時下降,48 h接著增高,72 h達第二次高峰。MVN區(qū)雙側(cè)在6、12、24、48、72、96、120 h存在著顯著差異(p0.05,n=6)。MVN區(qū)144h后c-Fos免疫陽性神經(jīng)元基本消失。4.UL后,MVN中c-Fos蛋白的表達:損傷同側(cè)24,12h達高峰,144h基本消失;損傷6 h后不對稱表達明顯高于損傷同側(cè),12小時下降,48 h接著增高,72 h達第二次高峰。MVN區(qū)雙側(cè)在6、12、24、48、72、96 h、120 h存在著顯著差異(p0.05,n=6)。MVN區(qū)144h后c-Fos蛋白基本消失。5.UL 72 h側(cè)腦室注射MK-801后,MVN內(nèi)c-Fos蛋白的表達具有顯著性差異(p0.05,n=6)。以上結(jié)果提示:1.清醒大鼠前庭代償過程中可能有Glu的參與;2.清醒大鼠前庭代償過程中會引起c-Fos蛋白的表達變化;3.清醒大鼠前庭代償過程Glu可能通過NMDA受體介導(dǎo)神經(jīng)信號的傳遞。
[Abstract]:The vestibular organ can feel the spatial position and the motion state. The vestibular receptor changes the body movement and the head space position into the afferent signal. The vestibular nerve fibers were transmitted to the vestibular nucleus vestibular nucleus via the vestibular nerve fiber pathway to receive the nerve signals from the vestibular organs. And together with vision and proprioceptive sensation to maintain the balance of the body. Unilateral vestibular labyrinth destruction after unilateral abyrinthe tomyULL. Vestibular compensation is an ideal experimental model to study the plasticity of central nervous system after pathological changes. Eye tremor and horizontal rotation. But vestibular dysfunction signs and symptoms will gradually disappear with the passage of time, called vestibular compensation. Park Bing-lin of Korea observed asymmetric expression of c-Fos in bilateral medial vestibular nucleus during the acute phase of vestibular compensation in anesthetized rats which destroyed peripheral vestibular organs. The asymmetric expression disappeared at about 6 days. We found that the afferent of vestibular signal was blocked shortly after 2% lidocaine was infused into the single lateral ear of anesthetized rats. There was asymmetric expression of glutamate in medial vestibular neuclei of the medial vestibular nucleus. These results suggest that Glu may be associated with vestibular compensation. However, so far, these experiments have been carried out in anesthetized animals, and there is no systematic research report on the changes of glutamate content in MVN region at different stages of vestibular compensation. This study was designed to use micronucleus dialysis, high performance liquid analysis, immunohistochemistry and Western blotting in awake animals. To observe the dynamic changes of Glu content and c-Fos protein in MVN region of conscious rats in different periods of unilateral destruction of peripheral vestibular organs. Glu and its NMDA receptors were involved in the process of vestibular compensation. The results were as follows: 1. After 1. 1. The head tilted to the injured side 6 hours after UL, and began to increase at 24 hours. Reaching the peak at 72 h did not relieve, but the eye tremor reached the peak at 24 h and reached the peak at 48 h, and disappeared at 120 h, and the horizontal rotation weakened after 6 h damage and 12 h. The content of Glu in the extracellular fluid of MVN region disappeared at 96 h and disappeared at 168h. 2. The content of Glu in the extracellular fluid of MVN region: the ipsilateral side reached the peak at 24 h and reached the peak at 144h. The contralateral side reached the peak at 6 h, reached the peak at 12 h and decreased at 48 h, then increased at 72 h and reached the second peak at 144 h. At 96 h, there was a significant difference in the number of c-Fos immunoreactive neurons in the MVN region after p0.05. 3. The number of c-Fos immunoreactive neurons in the MVN region reached its peak at 24 h after injury. 96 h recovery; The contralateral side reached the peak at 6 h and decreased at 12 h and decreased at 48 h, then increased to the second peak at 72 h. There was a significant difference at 120 h after the disappearance of c-Fos immunoreactive neurons. The expression of c-Fos protein in MVN: the ipsilateral injury reached the peak at 24 h and reached the peak at 14 4 h. The asymmetrical expression of MVN was significantly higher than that of ipsilateral injury at 12 hours and 48 hours after injury, and then increased to the second peak at 72 hours. There was significant difference between 96 h and 120 h (p 0.05). The c-Fos protein disappeared basically at 144h after 72 h injection of MK-801 into the lateral ventricle. There was significant difference in the expression of c-Fos protein in MVN. The above results suggested that Glu might be involved in the vestibular compensatory process of conscious rats. 2.The expression of c-Fos protein was changed during the process of vestibular compensation in conscious rats. 3. During the vestibular compensatory process, Glu may mediate neural signal transduction by NMDA receptor.
【學(xué)位授予單位】：延邊大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R33

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