【摘要】： 目的通過對(duì)乙肝疫苗接種后不同免疫應(yīng)答人群T細(xì)胞亞群的檢測(cè),探討乙肝疫苗接種后免疫應(yīng)答與T細(xì)胞亞群活化的關(guān)系。 方法采集18例乙肝疫苗接種應(yīng)答者(應(yīng)答者)、22例乙肝疫苗接種無應(yīng)答者(無應(yīng)答者)、10例未接受乙肝疫苗接種的健康對(duì)照者(對(duì)照者)全血,用流式細(xì)胞術(shù)對(duì)其外周血單核細(xì)胞的表面標(biāo)志物:CD69、CD4、CD8、CD25等進(jìn)行檢測(cè)。計(jì)算不同T細(xì)胞亞群百分比,如CD69+、CD4+、CD8+T細(xì)胞百分比及CD4+CD25+Treg細(xì)胞數(shù)。 結(jié)果 1、應(yīng)答者,無應(yīng)答者,對(duì)照者CD4+CD69+T細(xì)胞占CD4+T細(xì)胞的百分比分別為1.81±1.33%、0.64±0.48%、和0.56±0.39%,應(yīng)答者CD4+T細(xì)胞中CD69的表達(dá)明顯高于對(duì)照者(P＜0.01),也明顯高于無應(yīng)答者。但無應(yīng)答者與對(duì)照者間差異無顯著性意義(p＞0.05)。各組CD8+CD69+T細(xì)胞占CD8+T細(xì)胞的百分比分別為0.43±0.25%、3.05±1.69%、和3.11±2.41%,應(yīng)答者與對(duì)照者和無應(yīng)答者比較,差異均有非常顯著性意義(p＜0.01)。 2、應(yīng)答者,無應(yīng)答者,對(duì)照者CD3+CD4+T細(xì)胞占CD3+T細(xì)胞的百分比分別為50.84±6.36%、53.67±6.68%、和51.02±9.30%,各組結(jié)果比較,差異沒有顯著性意義(p＞0.05);各組CD3+CD8+T細(xì)胞占CD3+T細(xì)胞的百分比分別為41.02±4.41%、42.69±7.35%、和42.36±8.34%,統(tǒng)計(jì)學(xué)分析,組間結(jié)果的差異無顯著性意義(p＞0.05)。 3、應(yīng)答者,無應(yīng)答者,對(duì)照者CD4+CD25+Treg細(xì)胞占CD4+T細(xì)胞的百分比分別為0.59±0.46%、0.77±0.40%、和1.30±1.44%,統(tǒng)計(jì)學(xué)分析,組間結(jié)果的差異無顯著性意義(p＞0.05)。 結(jié)論 1.乙肝疫苗接種后無應(yīng)答與Th細(xì)胞早期活化不足有關(guān)。 2.乙肝疫苗接種后無應(yīng)答可能與抑制性T細(xì)胞活化增加有關(guān)。 3.CD4+CD25+Treg細(xì)胞也可能參與了乙肝疫苗接種應(yīng)答的負(fù)性調(diào)節(jié),但由于研究結(jié)果差異無統(tǒng)計(jì)學(xué)顯著性意義,其確切的意義還需進(jìn)一步擴(kuò)大樣本數(shù)量進(jìn)行研究。
[Abstract]:Objective to investigate the relationship between immune response and activation of T cell subsets in different immune responders after hepatitis B vaccine vaccination. Methods whole blood samples were collected from 18 hepatitis B vaccine responders (responders), 22 hepatitis B vaccinated non-responders (non-responders) and 10 healthy controls (controls) who were not vaccinated with hepatitis B vaccine, and the whole blood samples were collected from 18 hepatitis B vaccine responders (respondents), 22 hepatitis B vaccine non-responders (non-responders) and 10 healthy controls (controls). The surface markers of peripheral blood monocytes, such as CD69,CD4,CD8,CD25, were detected by flow cytometry. The percentages of different T cell subsets, such as CD69, CD4, CD8 T cells and the number of CD4 CD25 Treg cells, were calculated. Results 1. The percentage of CD4 CD69 T cells in CD4 T cells was 1.81 鹵1.33%, 0.64 鹵0.48% and 0.56 鹵0.39%, respectively. The expression of CD69 in CD4 T cells of responders was significantly higher than that of controls (P < 0.01), and it was also significantly higher than that of non-responders. However, there was no significant difference between the non-responders and the controls (p > 0.05). The percentage of CD8 CD69 T cells in CD8 T cells in each group was 0.43 鹵0.25%, 3.05 鹵1.69% and 3.11 鹵2.41%, respectively. there was significant difference between the responders and the controls and the non-responders (p < 0.01). 2, the percentage of CD3 CD4 T cells in CD3 T cells was 50.84 鹵6.36%, 53.67 鹵6.68%, and 51.02 鹵9.30%, respectively. there was no significant difference between the two groups (p > 0.05), and the percentage of CD3 T cells in control group was 50.84 鹵6.36%, 53.67 鹵6.68% and 51.02 鹵9.30%, respectively. there was no significant difference between the two groups (p > 0.05). The percentage of CD3 CD8 T cells in CD3 T cells in each group was 41.02 鹵4.41%, 42.69 鹵7.35% and 42.36 鹵8.34%, respectively. there was no significant difference between the two groups. 3, the percentage of CD4 CD25 Treg cells in CD4 T cells was 0.59 鹵0.46%, 0.77 鹵0.40% and 1.30 鹵1.44%, respectively. There was no significant difference in the results between the two groups (p > 0.05). Conclusion 1. The lack of response after hepatitis B vaccination was related to the lack of early activation of Th cells. 2. No response after hepatitis B vaccination may be related to the increase of inhibitory T cell activation. 3.CD4 CD25 Treg cells may also be involved in the negative regulation of hepatitis B vaccination response, but because there is no significant difference in the results of the study, its exact significance needs to be further expanded to study the number of samples.
【學(xué)位授予單位】：中南大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2008
【分類號(hào)】：R186

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