【摘要】： 對2004~2006年吉林省麻疹流行病學、麻疹野病毒病原學進行研究。對麻疹發(fā)病和防控措施進行描述流行病學分析;采用RT-PCR擴增所分離的19株麻疹野病毒N基因羧基末端450個核苷酸片斷,對其測序、分析,構(gòu)建基因親緣性關(guān)系樹,進行核苷酸變異、氨基酸變異和遺傳距離比較,明確了引起吉林省麻疹爆發(fā)、流行的麻疹野病毒是Hla基因亞型;對所分離的麻疹野病毒與Edmonston標準株、疫苗株進行血清學研究,探討不同人群血清對麻疹野病毒的中和作用,評價麻疹疫苗免疫保護效果。 人群不按免疫程序接種疫苗,導致人群對麻疹免疫力下降,由于麻疹野病毒抗原性的改變使疫苗免疫保護作用下降,均可能是導致吉林省三年麻疹爆發(fā)和流行的主要原因。
[Abstract]:Measles epidemiology and measles wild virus etiology in Jilin Province from 2004 to 2006 were studied. Descriptive epidemiological analysis of measles incidence and control measures was carried out. The carboxyl terminal 450 nucleotide fragments of N gene of 19 measles wild virus strains were amplified by RT-PCR and sequenced, analyzed and constructed. The nucleotide variation, amino acid variation and genetic distance of 19 measles wild virus strains were compared with each other, and the nucleotide variation, amino acid variation and genetic distance were compared. It is clear that measles wild virus is a subtype of Hla gene which causes measles outbreak in Jilin Province. The serological study of measles wild virus, Edmonston standard strain and vaccine strain was carried out to explore the neutralizing effect of sera from different populations on measles wild virus, and to evaluate the protective effect of measles vaccine against measles wild virus. The population was not vaccinated according to the immunization procedure, which resulted in the decrease of the population's immunity to measles. Because of the change of the antigenicity of wild measles virus, the immune protective effect of the vaccine decreased, which may be the main cause of the outbreak and epidemic of measles in Jilin Province in the past three years.
【學位授予單位】：吉林大學
【學位級別】：博士
【學位授予年份】：2007
【分類號】：R181.3;R373

【引證文獻】

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1 尚曉麗;夏曉玲;侯宗柳;杜曾慶;;昆明地區(qū)麻疹野病毒株基因型檢測[J];生物醫(yī)學工程與臨床;2012年02期

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相關(guān)碩士學位論文 前1條

1 尚曉麗;昆明地區(qū)麻疹病毒的分子流行病學研究[D];昆明醫(yī)學院;2008年

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