【摘要】：研究目的： 1．研究廣州、江門(mén)地區(qū)5歲以下嬰幼兒腹瀉諾瓦克樣病毒(NLVs)的感染情況、流行優(yōu)勢(shì)株及分布特征。 2．初步研究成人腹瀉NLVs的感染情況及病毒基因型別。 3．探討NLVs感染腹瀉的臨床特征及危險(xiǎn)因素。 方法： 1．用酶聯(lián)免疫吸附試驗(yàn)(ELISA)檢測(cè)輪狀病毒，對(duì)輪狀病毒陰性標(biāo)本用逆轉(zhuǎn)錄聚合酶鏈反應(yīng)(RT-PCR)方法檢測(cè)NLVs，并對(duì)部分NLVs陽(yáng)性標(biāo)本PCR產(chǎn)物進(jìn)行純化、測(cè)序，結(jié)合參考株相應(yīng)的核苷酸序列進(jìn)行進(jìn)化分析，判斷NLVs的流行優(yōu)勢(shì)株。 2．對(duì)NLVs感染腹瀉臨床特征的探討采用與輪狀病毒感染腹瀉進(jìn)行頻數(shù)匹配的兩樣本比較研究；危險(xiǎn)因素的研究采用1：1配對(duì)的病例對(duì)照研究。數(shù)據(jù)管理采用Epidata2.1a進(jìn)行雙份錄入，SPSS12.0進(jìn)行統(tǒng)計(jì)分析，，定性資料采用X~2檢驗(yàn)，定量資料采用t檢驗(yàn)或非參數(shù)檢驗(yàn)。NLVs感染腹瀉發(fā)病的危險(xiǎn)因素采用SAS8.2進(jìn)行單因素和多因素1：1條件logistic回歸分析。 結(jié)果： 1．廣州、江門(mén)兩地NLVs的檢出率分別為23.49％(70／298)和17.36％(25／144)，主要感染2歲以下的嬰幼兒，季節(jié)中的各個(gè)月份分布相當(dāng)。33株測(cè)序病毒30株屬于GⅡ-4型，3株屬于GⅡ-3型。35例成人腹瀉標(biāo)本檢出4株NLVs，測(cè)序的2株分別屬于GⅡ-3和GⅡ-4型。
[Abstract]:Objective: 1. To study the prevalence, prevalence and distribution of Norwalk like virus (NLVs) in infants under 5 years old in Guangzhou and Jiangmen area. 2. To study the infection of NLVs and the type of virus gene in adult diarrhea. 3. To investigate the clinical characteristics and risk factors of NLVs infection diarrhea. Methods: 1. Rotavirus was detected by enzyme linked immunosorbent assay (ELISA) and NLVs, was detected by reverse transcriptase polymerase chain reaction (RT-PCR) in rotavirus negative specimens. The PCR products of some NLVs positive specimens were purified, sequenced, and the corresponding nucleotide sequences of the reference strains were analyzed to determine the prevailing dominant strains of NLVs. 2. To investigate the clinical characteristics of diarrhea infected with NLVs, two samples matched with rotavirus infection diarrhea were used to compare the clinical characteristics and the risk factors were studied by a 1:1 matched case-control study. In data management, Epidata2.1a is used for double input, SPSS12.0 for statistical analysis, and qualitative data for X2 test. The risk factors of NLVs infection diarrhea were analyzed by SAS8.2 with single factor and multivariate 1:1 conditional logistic regression analysis. Results: 1. The positive rate of NLVs in Guangzhou and Jiangmen was 23.49% (70 / 298) and 17.36% (25 / 144) respectively. 33 strains of sequenced viruses belong to G 鈪

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