【摘要】：目的研究錯配修復系統(tǒng)MLH1基因rs1800734多態(tài)性與肝細胞癌遺傳易感性的關系。方法采用以醫(yī)院為基礎的病例對照研究方法,運用熒光定量聚合酶鏈反應(polymerase chain reaction,PCR)和非條件Logistic回歸分析MLH1基因型在兩組中分布頻率的差異,以及基因多態(tài)性和環(huán)境因素的交互作用。結果病例組MLH1基因位點AA、AG和GG各基因型頻率分別為37.09%、48.57%和14.35%,對照組中基因型頻率分別為32.89%、47.46%和19.65%,差異無統(tǒng)計學意義(P=0.085)。多因素Logistic回歸分析表明,與AA基因型相比,AG或者GG基因型的個體罹患HCC的風險OR值分別為1.102和1.544。病例組等位基因(A)頻率(61.37%)較對照組(56.62%)增高(醊2=4.22,P=0.040),A等位基因攜帶者患肝細胞癌的危險性是G等位基因攜帶者的1.217倍。交互作用分析結果表明MLH1基因多態(tài)性與腫瘤家族史、乙型肝炎病毒表面抗原(epatitis B surface antigen,HBsAg)陽性之間均存在交互作用(P0.001),OR值分別為4.763和16.967。結論 MLH1基因可能是廣西人群肝細胞癌的危險因素之一,其多態(tài)性與腫瘤家族史、HBsAg陽性之間在肝細胞癌發(fā)生中存在交互作用,能增加罹患肝細胞癌的風險。
[Abstract]:Objective to study the relationship between rs1800734 polymorphism of MLH1 gene and susceptibility to hepatocellular carcinoma (HCC). Methods A hospital-based case-control study was conducted. The frequency of MLH1 genotypes in the two groups was analyzed by fluorescence quantitative polymerase chain reaction (polymerase chain reaction,PCR) and non-conditional Logistic regression analysis. And the interaction between genetic polymorphism and environmental factors. Results the frequencies of AA,AG and GG genotypes in MLH1 gene locus were 37.09% and 14.35%, respectively. In the control group, the frequencies of AA,AG and GG genotypes were 32.89% and 19.65%, respectively. The difference was not statistically significant (P0. 085). Multivariate Logistic regression analysis showed that the OR values of the individuals with AG or GG genotype for HCC were 1.102 and 1.544, respectively, compared with the AA genotype. The allele (A) frequency (61.37%) in the case group was 1.217 times higher than that in the control group (56.62%). The results of interaction analysis showed that there was interaction between MLH1 gene polymorphisms and tumor family history, hepatitis B virus surface antigen (epatitis B surface antigen,HBsAg) positive (P0. 001), OR values were 4.763 and 16.967 respectively. Conclusion MLH1 gene may be one of the risk factors of hepatocellular carcinoma in Guangxi population. There is interaction between polymorphism and family history and HBsAg positive in the occurrence of hepatocellular carcinoma, which can increase the risk of hepatocellular carcinoma.
【作者單位】： 桂林醫(yī)學院公共衛(wèi)生學院流行病與衛(wèi)生統(tǒng)計學教研室;廣西醫(yī)科大學公共衛(wèi)生學院流行病與衛(wèi)生統(tǒng)計學教研室;
【基金】：國家自然科學青年基金(81001285) 國家自然科學地區(qū)基金(81160359) 廣西教育廳項目(200911MS162)
【分類號】：R735.7;R181.1

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相關期刊論文 前4條

1 ;Synergistic Action of Clonorchiasis,HBV Infection and Alcohol Consumption on Primary Hepatocellular Carcinoma[J];Clinical Oncology and Cancer Research;2009年02期

2 曾小云;余紅平;仇小強;紀龍;李龍Z，

本文編號：2374567