【摘要】：目的研究錯(cuò)配修復(fù)系統(tǒng)MLH1基因rs1800734多態(tài)性與肝細(xì)胞癌遺傳易感性的關(guān)系。方法采用以醫(yī)院為基礎(chǔ)的病例對(duì)照研究方法,運(yùn)用熒光定量聚合酶鏈反應(yīng)(polymerase chain reaction,PCR)和非條件Logistic回歸分析MLH1基因型在兩組中分布頻率的差異,以及基因多態(tài)性和環(huán)境因素的交互作用。結(jié)果病例組MLH1基因位點(diǎn)AA、AG和GG各基因型頻率分別為37.09%、48.57%和14.35%,對(duì)照組中基因型頻率分別為32.89%、47.46%和19.65%,差異無(wú)統(tǒng)計(jì)學(xué)意義(P=0.085)。多因素Logistic回歸分析表明,與AA基因型相比,AG或者GG基因型的個(gè)體罹患HCC的風(fēng)險(xiǎn)OR值分別為1.102和1.544。病例組等位基因(A)頻率(61.37%)較對(duì)照組(56.62%)增高(醊2=4.22,P=0.040),A等位基因攜帶者患肝細(xì)胞癌的危險(xiǎn)性是G等位基因攜帶者的1.217倍。交互作用分析結(jié)果表明MLH1基因多態(tài)性與腫瘤家族史、乙型肝炎病毒表面抗原(epatitis B surface antigen,HBsAg)陽(yáng)性之間均存在交互作用(P0.001),OR值分別為4.763和16.967。結(jié)論 MLH1基因可能是廣西人群肝細(xì)胞癌的危險(xiǎn)因素之一,其多態(tài)性與腫瘤家族史、HBsAg陽(yáng)性之間在肝細(xì)胞癌發(fā)生中存在交互作用,能增加罹患肝細(xì)胞癌的風(fēng)險(xiǎn)。
[Abstract]:Objective to study the relationship between rs1800734 polymorphism of MLH1 gene and susceptibility to hepatocellular carcinoma (HCC). Methods A hospital-based case-control study was conducted. The frequency of MLH1 genotypes in the two groups was analyzed by fluorescence quantitative polymerase chain reaction (polymerase chain reaction,PCR) and non-conditional Logistic regression analysis. And the interaction between genetic polymorphism and environmental factors. Results the frequencies of AA,AG and GG genotypes in MLH1 gene locus were 37.09% and 14.35%, respectively. In the control group, the frequencies of AA,AG and GG genotypes were 32.89% and 19.65%, respectively. The difference was not statistically significant (P0. 085). Multivariate Logistic regression analysis showed that the OR values of the individuals with AG or GG genotype for HCC were 1.102 and 1.544, respectively, compared with the AA genotype. The allele (A) frequency (61.37%) in the case group was 1.217 times higher than that in the control group (56.62%). The results of interaction analysis showed that there was interaction between MLH1 gene polymorphisms and tumor family history, hepatitis B virus surface antigen (epatitis B surface antigen,HBsAg) positive (P0. 001), OR values were 4.763 and 16.967 respectively. Conclusion MLH1 gene may be one of the risk factors of hepatocellular carcinoma in Guangxi population. There is interaction between polymorphism and family history and HBsAg positive in the occurrence of hepatocellular carcinoma, which can increase the risk of hepatocellular carcinoma.
【作者單位】： 桂林醫(yī)學(xué)院公共衛(wèi)生學(xué)院流行病與衛(wèi)生統(tǒng)計(jì)學(xué)教研室;廣西醫(yī)科大學(xué)公共衛(wèi)生學(xué)院流行病與衛(wèi)生統(tǒng)計(jì)學(xué)教研室;
【基金】：國(guó)家自然科學(xué)青年基金(81001285) 國(guó)家自然科學(xué)地區(qū)基金(81160359) 廣西教育廳項(xiàng)目(200911MS162)
【分類號(hào)】：R735.7;R181.1

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相關(guān)期刊論文 前4條

1 ;Synergistic Action of Clonorchiasis,HBV Infection and Alcohol Consumption on Primary Hepatocellular Carcinoma[J];Clinical Oncology and Cancer Research;2009年02期

2 曾小云;余紅平;仇小強(qiáng);紀(jì)龍;李龍Z，

本文編號(hào)：2374567