本文關(guān)鍵詞：HIF-1α，VEGF及PIGF在不同海拔世居藏族及移居漢族的水平及意義的研究，由筆耕文化傳播整理發(fā)布。

        目的：本研究旨在通過測定世居藏族、移居漢族及平原漢族缺氧誘導(dǎo)因子-1α（hypoxia-induble factor-1α，HIF-1α），血管內(nèi)皮生長因子（vascularendothelial growth factor，VEGF）及胎盤生長因子（placenta growthfactor，PIGF）的水平，分析幾種因子在不同海拔藏漢族之間的差異，幾種因子之間及它們與動脈血氧分壓（arterial partial pressure of oxygen，PaO2）的相關(guān)性，了解藏族、漢族對高原低氧環(huán)境的適應(yīng)特征，探討HIF-1α，VEGF及PIGF在高原低氧適應(yīng)中所起的作用。方法：選取平原（海拔5m）健康成年漢族，中度海拔（2261m）、高海拔（4380m）健康成年藏、漢族男性（20-30歲）作為研究對象，分為平原組（1組）、中度海拔移居漢族組（2組）、中度海拔世居藏族組（3組）、高海拔移居漢族組（4組）、高海拔世居藏族組（5組），每組各20名。移居者在高原居住時間為2年以上。中、高度海拔藏漢族組均進(jìn)行慢性高原�。–MS）評分均小于5分。所有受試者均簽署知情同意書。受試者檢驗血?dú)夥治黾把t蛋白。空腹抽取肘前靜脈血分離血清置于-80℃冰箱保存,應(yīng)用ELISA法測定血清中HIF-1α，VEGF,PIGF的水平。比較各組HIF-1α，VEGF,PIGF的水平，因子之間及與PaO2的相關(guān)性。結(jié)果：1.各組間PaO2，Hb的比較（見表1）。PaO2：5組間PaO2比較有顯著性差異，2，3,4,5組均低于1組（P<0.01）；4,5組低于2,3組（P<0.01）。但2組與3組之間無顯著性差異（P>0.05）；4組與5組之間無顯著性差異（P>0.05）。Hb：5組間Hb比較有顯著性差異,4、5組低于1、2、3組（P<0.01），5組低于4組（P<0.01），1組、2組、3組之間無顯著性差異（P>0.05）。2.各組間血清HIF-1α，VEGF及PIGF水平比較（見表2）。HIF-1α：5組間HIF-1α比較有顯著性差異，4組低于1、2、3組（P<0.05），5組低于3組（P<0.05），但1組、2組、3組之間無顯著性差異（P>0.05）；4組、5組之間無顯著性差異（P>0.05）VEGF：5組間VEGF比較有顯著性差異，4組低于1、2、3組（P<0.05），5組低于1、3、5組（P<0.05），但1組、2組、3組之間無顯著性差異（P>0.05）；2組、5組之間無顯著性差異（P>0.05）。PIGF：5組間PIGF比較有顯著性差異，2、3、4組低于1組（P<0.05），4、5組低于2、3組（P<0.05），5組低于4組（P<0.05），但2、3組之間無顯著性差異（P>0.05）。3.血清HIF-1α，VEGF及PIGF水平與PaO2相關(guān)性HIF‐1α，VEGF，PIGF和PaO2負(fù)相關(guān)，有統(tǒng)計學(xué)意義（r分別為－0.475，－0.686，－0.511，P<0.01）。4.血清PIGF與HIF-1α，VEGF相關(guān)性PIGF和HIF‐1α，VEGF正相關(guān)，有統(tǒng)計學(xué)意義（r分別為0.401，0.440，P<0.01）。結(jié)論：1.隨著海拔的升高，各組PaO2逐漸降低，但在同一海拔的藏漢族之間PaO2無明顯差異。Hb蛋白的升高僅在高海拔藏漢族出現(xiàn)，且藏族顯著低于漢族。提示缺氧到一定程度才會引起Hb的升高，藏族較漢族具有更好的低氧適應(yīng)能力。2.HIF-1α在高度海拔才明顯升高，但高海拔藏漢族之間無顯著差異，提示HIF-1α在低氧到一定程度時水平增加，，藏、漢族在同一低氧水平時HIF-1α的水平是相同的。4.VEGF在中度海拔無顯著升高，在高海拔顯著升高，高海拔藏族與漢族之間有顯著差異，說明漢族在低氧刺激下血管內(nèi)皮生長因子增生更為活躍。5.PIGF在中度海拔藏漢族顯著低于平原組，高海拔漢族顯著高于中度海拔藏漢族，高海拔藏族顯著低于中度海拔藏漢族及高海拔漢族，而與平原組無顯著差異。推測PIGF在輕度低氧處于抑制狀態(tài)，低氧到一定程度才會升高，漢族明顯高于藏族。6.HIF-1α,VEGF,PIGF與PaO2呈負(fù)相關(guān)，PIGF與HIF-1α，VEGF呈正相關(guān)。

    Objective：To measure the level of hypoxia inducible factor-1α（HIF-1α） vascularendothelial growth factor （VEGF）and placenta growth factor （PIGF） of the serum inTibetan, the moved Han and Han in plain. To analyse the difference of them at thedifferent altitude, the correlation among them and the correlation between HIF-1α,HIF-2α with arterial partial pressure of oxygen （PaO2）.In order to understand theadaptability of Tibetan and Han to high altitude. To investigate the significance ofHIF-1α,VEGF and PIGF in different altitude.Methods：Chooses healthy adult of Han in plain （altitude5m,group1）, in moderatealtitude （2261m, group2）and high altitude （4380m,group4）, healthy adult ofTibetan in moderate altitude （2261m, group3） and high altitude （4380m,group5）.The age of them between of20and30. Each group has20men. Migrants live inplateau more than2years. Tibetan and Han of high altitude were done the chronicmountain sickness scores（CMS）.Their scores were all less than five points. Everysubject signed the informed agreement. All of them measured hemoglobin andarterial blood gas analysis. Extracted venous blood, separated serum and saved in-80℃refrigerator. The level of HIF-1α,VEGF and PIGF were determined withELISA.Compared the difference within groups.Analyzed the correlation amongthese three factors and them with PaO2.Results：1. The comparison of the Hb and PaO2among each group （table1）.PaO2: There was significant difference of PaO2among the five groups（P <0.01）.ThePaO2of group1was higher than other groups（P <0.01）. The PaO2of group4andgroup5were lower than group2and group3（P <0.01）. But PaO2between group2and group3didn’t have significant difference （P>0.05）.The PaO2between group4and group5also didn’t have significant difference （P>0.05）.Hb （g/dl）: There was significant difference among the five groups.Hb of group4and group5were higher than other groups（P <0.01）. Hb of group5was lower thangroup4（P <0.01）. Hb among group1, group2and group3didn’t have significantdifference （P>0.05）.2. The comparison of HIF-1α,VEGF and PIGF level among each group （see table2）.HIF-1α: There was significant difference among the five groups. HIF-1α of group4was higher than other groups （P <0.05）.HIF-1α of group5was higher than the thirdgroup （P <0.05）.There was no significant difference among group1, group2andgroup3.（P>0.05）.VEGF:There was significant difference Among5groups.The level of VEGF waslower than group1,2and3（P<0.05）, group5was lower than group1,3and5（P<0.05）.There was no significant difference among group1, group2and group3（P>0.05）, between group2and group5were also no significant difference （P>0.05）;PIGF: There was significant difference among5groups. Group2and3were lowerthan group1（P<0.05）,group4was highest in all group（sP<0.05）. Group5was lowerthan group4（P<0.05），higher than group2and3,no significant difference withgroup1（P>0.05）.3. The correlation of HIF-1α, VEGF and PIGF with PaO2.There were negative correlation between HIF-1α,VEGF and PIGF with PaO2（requals-0.291,-0.686,-0.511respectively,P <0.01）.4. The correlation between PIGF with HIF-1α and VEGF.PIGF positive correlated with HIF-1α and VEGF （r equals0.401,0.440respe-ctively,P <0.01）Conclusions：1.With the rise of altitude, the PaO2decreased.It is the same level ofPaO2in the same elevation in Han and Tibetan. Hb increases only in high altitude,theHb of Tibetan is significantly lower than Han. That means only when hypoxiadecreased to certain degree, the Hb will increase.Tibetan has better hypoxiaadaptability than Han.2. In high altitude,HIF-1α increased significantly, but there is no significantdifference between Han and Tibetan. It’s tip that HIF-1doesn’t increase until oxygento a certain degree in the same low oxygen levels,HIF-1α is identical. 3.VEGF increased in high altitude, there was significant difference betweenTibetan and Han.That indicated that VEGF is sensitive to hypoxia in Han thanTibetan.4.PIGF decreased in moderate high altitude than plain.PIGF of Han in highaltitude was higher than Tibetan and Han in moderate altitude.PIGF of Tibetan waslower than moderate altitude and Han in high altitude,but there was no significantdifference with plain.That may be indicated that PIGF was depressed by mildhypoxia,it will increase only when hypoxia to a certain degree, Han was higher thanTibetan.5.There were negative correlation between HIF-1α,VEGF and PIGF with PaO2.PIGF positive correlated with HIF-1α and VEGF.

        HIF-1α，VEGF及PIGF在不同海拔世居藏族及移居漢族的水平及意義的研究

中文摘要5-7Abstract7-9前言10-11材料與方法11-14結(jié)果14-16討論16-18結(jié)論18-19參考文獻(xiàn)19-22附錄22-23致謝23-24綜述24-33    參考文獻(xiàn)30-33攻讀學(xué)位期間取得的研究成果33

  本文關(guān)鍵詞：HIF-1α，VEGF及PIGF在不同海拔世居藏族及移居漢族的水平及意義的研究，由筆耕文化傳播整理發(fā)布。