【摘要】：70年代美國(guó)爆發(fā)“軍團(tuán)病”以來,已經(jīng)有多個(gè)國(guó)家有“軍團(tuán)病”的報(bào)道。我國(guó)南京在1982年首次證實(shí)軍團(tuán)菌病病例。多個(gè)城市對(duì)空調(diào)冷卻塔進(jìn)行軍團(tuán)菌調(diào)查,發(fā)現(xiàn)其中軍團(tuán)菌的陽性率為50%-80%。2003年中國(guó)經(jīng)歷SARS的襲擾后,人們對(duì)空調(diào)系統(tǒng)的衛(wèi)生狀況表現(xiàn)出空前的關(guān)注。本課題擬對(duì)深圳市空調(diào)冷卻塔微生物污染狀況,尤其是軍團(tuán)菌污染狀況作較為全面的調(diào)查,建立流行病學(xué)資料。 目的:1.對(duì)深圳市賓館、醫(yī)院、影院、寫字樓等公共場(chǎng)所的空調(diào)冷卻塔水進(jìn)行軍團(tuán)菌的分離培養(yǎng),了解軍團(tuán)菌的污染狀況。檢測(cè)細(xì)菌總數(shù)、大腸菌群、糞大腸菌群,初步了解空調(diào)冷卻塔微生物污染狀況以及軍團(tuán)菌與它們之間是否存在一定聯(lián)系。2.對(duì)所采集的冷卻塔水進(jìn)行PCR檢測(cè),并與常規(guī)分離培養(yǎng)方法進(jìn)行比較。3.用AFLP技術(shù)對(duì)常規(guī)分離培養(yǎng)出的菌株進(jìn)行分子生物學(xué)分型,分析分離菌株的多態(tài)性,確定菌株的遺傳基礎(chǔ)之間相互的聯(lián)系和變異,了解深圳市軍團(tuán)菌分布流行病學(xué)特征。 方法:1.采用平皿計(jì)數(shù)法對(duì)中央空調(diào)冷卻塔水進(jìn)行軍團(tuán)菌的分離培養(yǎng),用血清學(xué)方法對(duì)分離菌株進(jìn)行初步鑒定。2.檢測(cè)冷卻塔水中
[Abstract]:Since the outbreak of Legionnaires disease in the United States in the 1970s, there have been reports of Legionnaires' Disease in many countries. Legionella was first confirmed in 1982 in Nanjing, China. Legionella in air conditioning cooling towers was investigated in many cities. It was found that the positive rate of Legionella was 50%-80%. After China experienced the disturbance of SARS in 2003, people showed unprecedented concern about the sanitary condition of air conditioning system. The aim of this paper is to investigate the microorganism pollution of air conditioning cooling tower in Shenzhen, especially Legionella, and to establish epidemiological data. Purpose 1. The air conditioning cooling tower water of Shenzhen hotels, hospitals, cinemas, office buildings and other public places were isolated and cultured to understand the contamination of Legionella. The total number of bacteria, coliform group, fecal coliform group were detected to understand the microorganism pollution in cooling tower of air conditioning system and whether there was a certain relationship between Legionella and them. The collected cooling tower water was detected by PCR and compared with conventional culture method. 3. 3. The molecular biological typing of the strains isolated by conventional methods was carried out by using AFLP technique, the polymorphism of the isolates was analyzed, the relationship and variation of the genetic basis of the strains were determined, and the epidemiological characteristics of the distribution of Legionella in Shenzhen were understood. Method 1: 1. Legionella was isolated and cultured in the cooling tower water of central air conditioning system by plate counting method. The isolated strain was preliminarily identified by serological method. Detection of cooling tower wate
【學(xué)位授予單位】：四川大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2005
【分類號(hào)】：R181.3

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