本文選題：巴爾通體 + 分子鑒定�。� 參考：《中國疾病預(yù)防控制中心》2010年碩士論文

【摘要】： 目的：調(diào)查特定人群(職業(yè)性動(dòng)物接觸人群、犬傷或貓傷人群)及不同來源的動(dòng)物(家養(yǎng)寵物、流浪動(dòng)物、實(shí)驗(yàn)動(dòng)物)巴爾通體感染狀況及流行病學(xué)信息,獲得不同宿主的巴爾通體分離株,了解并比較巴爾通體感染率及分布情況,探索巴爾通體感染的危險(xiǎn)因素。 方法：在山東省濰坊市和北京市采集193份人血樣與478份動(dòng)物血樣,并收集其相關(guān)的流行病學(xué)信息。將采獲的樣本抗凝血接種于含5%去纖維羊血的腦心浸液培養(yǎng)基上,置于37度、含5%CO2培養(yǎng)箱中分離培養(yǎng)。挑選巴爾通體疑似菌落,應(yīng)用聚合酶鏈反應(yīng)技術(shù)(PCR)在屬分類水平鑒定巴爾通體,選擇gltA、16S、16S-23S rRNA ITS、ftsZ、ribC引物進(jìn)行PCR擴(kuò)增并測序,將所測核酸序列進(jìn)行同源性比較及系統(tǒng)發(fā)育分析,確定巴爾通體種。提取血樣DNA并進(jìn)行g(shù)ltA、tRNA引物PCR擴(kuò)增、測序序列進(jìn)行同源性分析。利用抗巴爾通體抗體IgG檢測試劑盒檢測樣本血清中抗體水平。利用SPSS分析實(shí)驗(yàn)室結(jié)果與現(xiàn)場采集的流行病學(xué)信息之間關(guān)系。 結(jié)果： 1、不同人群之間(貂狐飼養(yǎng)者、犬傷或貓傷人群、實(shí)驗(yàn)動(dòng)物飼養(yǎng)人員)巴爾通體血清陽性率沒有顯著性差異,平均為33.7%。犬傷或貓傷人群巴爾通體血清陽性率有在不同地區(qū)顯著性差異,城區(qū)犬傷或貓傷人群血清陽性率(56.4%)明顯高于農(nóng)村地區(qū)(11.9%),且僅有貓接觸史的咬傷人群的血清陽性率(83.3%)顯著高于僅有犬接觸史的咬傷人群(25.8%)。不同類型動(dòng)物接觸史(家養(yǎng)寵物、職業(yè)性接觸、流浪動(dòng)物)、不同性別、不同單位、不同年齡、不同工齡的調(diào)查人群巴爾通體血清陽性率沒有顯著性差異。 2、本課題獲得的22株貓分離株全部為漢賽巴爾通體。流浪貓帶菌率(30.4%)明顯高于家養(yǎng)寵物貓(4.8%),但兩者血清陽性率沒有顯著性差異,平均為39.4%。寄生蟲感染貓的帶菌率(36.6%)要明顯高于無寄生蟲感染貓(5.9%)。幼貓帶菌率(27.6%)明顯高于性成熟貓(10.7%),不同年齡的貓帶菌情況不同,以一歲以內(nèi)的小貓帶菌率最高(27.9%)。不同品種、來源、性別、體重的貓之間巴爾通體感染狀況沒有顯著性差異。 3、間接免疫熒光法(IFA)在診斷貓現(xiàn)時(shí)感染時(shí),靈敏度(77.3%)和特異度(66.7%)一般。IFA的陰性預(yù)測值較高(94.8%),既當(dāng)IFA陰性時(shí),有較大的把握該貓不帶有菌血癥。 4、本課題獲得國內(nèi)首株漢賽巴爾通體犬分離株,犬一般感染文森巴爾通體,獲得漢賽巴爾通體犬分離株對巴爾通體的宿主研究提出方向。 5、本課題獲得國內(nèi)首株五日熱巴爾通體分離株,這也是國際首次在獼猴體內(nèi)發(fā)現(xiàn)自然感染的五日熱巴爾通體。這證實(shí)國內(nèi)存在戰(zhàn)壕熱病原體,提示巴爾通體的流行病學(xué)可能比預(yù)期的要復(fù)雜,對巴爾通體宿主特異性的進(jìn)一步研究提出方向。
[Abstract]:Objective: to investigate the prevalence and epidemiological information of Bartonella infection in specific population (occupational animal contact population, canine injury or cat injury population) and animals from different sources (domestic pets, stray animals, laboratory animals). Baltonella isolates from different hosts were obtained, and the infection rate and distribution of Baltonella were compared and the risk factors of Bartonella infection were explored. Methods: 193 human and 478 animal blood samples were collected in Weifang and Beijing, Shandong Province. The collected anticoagulant blood was inoculated on the medium containing 5% defibrillated sheep blood and incubated in 37 degrees in a 5%CO2 incubator. The suspected colony of Bartonella was selected and identified at the taxonomic level by polymerase chain reaction (PCR). The primers of gltAmin16Sn16S-23S rRNA ITSftsZribC were selected for PCR amplification and sequencing. Homology and phylogenetic analysis of the nucleic acid sequences were carried out. To determine the species of Bartonella. DNA was extracted from blood samples and amplified by PCR with gltAgna tRNA primer, and the sequence was sequenced for homology analysis. The antibody level in serum was detected by anti-Bartonella antibody IgG assay kit. The relationship between the laboratory results and the epidemiological information collected in the field was analyzed by SPSS. Results: 1. There was no significant difference in the positive rate of Baltonella among different populations (mink breeders, dogs or cats, laboratory animal breeders), with an average of 33.7. The positive rates of Baltonella in dogs and cats were significantly different in different areas. The positive rate of serum in dogs or cats in urban areas was significantly higher than that in rural areas (11.9%), and the positive rate of 83.3% in people with only cat contact history was significantly higher than that in the group with only dog contact history (25.8%). There was no significant difference in the positive rate of Baltonella among different types of animals (domestic pets, occupational contact, stray animals, different sex, different units, different ages and different years of service). 2. The 22 cat isolates in this study were all Hansebartonella. The bacterial rate of stray cats (30.4%) was significantly higher than that of domestic pet cats (4.8%), but there was no significant difference in serum positive rate between the two groups (mean 39.4%). The bacterial rate of parasitic infected cats was significantly higher than that of non-parasitic infected cats (36.6%). The rate of carrying bacteria in kittens was significantly higher than that in sexually mature cats (10.7%). The rate of carrying bacteria in kittens of different ages was different, and the highest rate of carrying bacteria was 27.9% in kittens under one year of age. There was no significant difference in Bartonella infection among cats of different breeds, sources, gender, and body weight. (3) the sensitivity and specificity of indirect immunofluorescence assay (IFA) in the diagnosis of current infection in cats were 77.3) and 66.7). The negative predictive value of IFA was higher than that of normal. When IFA was negative, there was a greater certainty that the cat did not have bacteremia. 4. In this study, the first strain of Hanseybaltonella canis was obtained, and the canine strain was generally infected with Venson Bartonella, and the direction of research on the host of Bartonella was put forward. In this study, the first 5-day hot Baltonella isolate was obtained in China, which was the first time that natural infection was found in rhesus monkeys. This confirms the existence of trench fever pathogens in China, suggesting that the epidemiology of Bartonella may be more complicated than expected.
【學(xué)位授予單位】：中國疾病預(yù)防控制中心
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2010
【分類號】：R181.3

【引證文獻(xiàn)】

相關(guān)期刊論文 前1條

1 李小麗;陰峧宏;;對巴爾通體感染的臨床認(rèn)識[J];中國病原生物學(xué)雜志;2012年11期

相關(guān)碩士學(xué)位論文 前1條

1 左雙燕;我國黑龍江林區(qū)鼠型動(dòng)物巴爾通體感染調(diào)查與分離鑒定[D];中南大學(xué);2012年

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本文編號：1906828