本文選題：鉛 + 淋巴細(xì)胞�。� 參考：《浙江大學(xué)》2012年碩士論文

【摘要】：目的應(yīng)用流式細(xì)胞術(shù)檢測γH2AX蛋白,結(jié)合人群調(diào)查和體外實驗,分析鉛致人淋巴細(xì)胞DNA雙鏈斷裂作用,探索流式細(xì)胞術(shù)檢測γH2AX以評價人群DNA雙鏈斷裂水平的可行性。方法選取某蓄電池廠工人67例和對照人群70例,采外周靜脈血提取淋巴細(xì)胞,利用流式細(xì)胞術(shù)檢測γH2AX蛋白,分析淋巴細(xì)胞DNA雙鏈斷裂水平；不同劑量、時間下醋酸鉛染毒健康人外周血淋巴細(xì)胞,利用流式細(xì)胞術(shù)檢測γH2AX蛋白,分析淋巴細(xì)胞DNA雙鏈斷裂水平。結(jié)果人群調(diào)查結(jié)果顯示高濃度組(41.76%±28.57%；9.90±3.35)、低濃度組(33.18%±30.64%；9.39±4.83)、內(nèi)對照組(35.87%±34.09%；10.04±5.77)DNA損傷率和平均熒光強度均高于外對照組(0.28%±0.28%；6.95±2.93),差異有統(tǒng)計學(xué)意義(P0.05)；性別、吸煙、飲酒、工齡對職業(yè)鉛暴露工人DNA損傷水平無影響(P0.05)；偏相關(guān)分析結(jié)果顯示內(nèi)對照組DNA損傷率和平均熒光強度與年齡存在相關(guān)性(r=-0.430、-0.391,P0.05),鉛暴露高濃度組平均熒光強度與血鉛、δ-ALA存在相關(guān)性(r=0.621、-0.697, P0.05)。體外實驗結(jié)果顯示1h和2h染毒組中除62.5μmol/L外,125μmol/L、250μmol/L、500μmol/LDNA損傷率均與陰性對照組、陽性對照組存在統(tǒng)計學(xué)差異(P0.01),隨著染毒劑量增高,DNA損傷率呈現(xiàn)先增高后降低趨勢；各濃度組吸光度值均低于陰性對照組,但未見劑量效應(yīng)關(guān)系,2h組吸光度值均低于1h組。結(jié)論鉛致DNA雙鏈斷裂損傷可能是DNA修復(fù)抑制、DNA斷裂、DNA-DNA交聯(lián)、DNA-蛋白質(zhì)交聯(lián)多方面作用因素共同結(jié)果,流式細(xì)胞術(shù)檢測yH2AX是一種值得運用于檢測大樣本DNA雙鏈斷裂水平的方法。
[Abstract]:Objective to detect 緯 H2AX protein by flow cytometry, analyze the double strand breaks of DNA in human lymphocytes induced by lead, and explore the feasibility of detecting 緯 H2AX by flow cytometry to evaluate the level of DNA double strand breaks in human lymphocytes. Methods 67 workers from a storage battery factory and 70 controls were selected to extract lymphocytes from peripheral venous blood. Flow cytometry was used to detect 緯 H2AX protein and analyze the level of DNA double strand breaks in lymphocytes. The 緯 H2AX protein was detected by flow cytometry and the level of DNA double strand break in lymphocytes was analyzed by flow cytometry. Results the results of population investigation showed that the damage rate and average fluorescence intensity of 5.77)DNA in high concentration group (41.76% 鹵28.576.95 鹵3.35), low concentration group (33.18% 鹵30.64 鹵9.39 鹵4.83) and internal control group (35.87% 鹵34.090.04 鹵10.04 鹵2.93g) were higher than those in external control group (0.28% 鹵0.286.95 鹵2.93g), the difference was significant (P 0.05). The results of partial correlation analysis showed that there was a correlation between the DNA damage rate and average fluorescence intensity and age in the control group, the average fluorescence intensity in the lead exposure group was correlated with the blood lead, the 未 -ALA level was correlated with r0.621-0.697, P0.05. The results of in vitro experiments showed that the damage rate of 125渭 mol / L ~ (125) 渭 mol / L ~ (250 渭 mol / L) ~ (500 渭 mol / L) mol/LDNA in 1 h and 2 h exposure group was significantly higher than that in the negative control group (P < 0.01), and the damage rate increased firstly and then decreased with the increase of dose. The absorbance value of each concentration group was lower than that of negative control group, but the absorbance value of 2 h group was lower than that of 1 h group. Conclusion DNA double strand break damage induced by lead may be the result of DNA repair inhibiting DNA-DNA cross-linking DNA-protein crosslinking. Flow cytometry is a valuable method to detect the level of DNA double strand breaks in large samples.
【學(xué)位授予單位】：浙江大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R181.3

【參考文獻】

相關(guān)期刊論文 前9條

1 易偉松;羅賢清;陳建軍;王海嬰;王芳;;骨鉛含量應(yīng)作為人體鉛中毒新的生物標(biāo)記[J];廣東微量元素科學(xué);2006年04期

2 舒為群;卓?波;;鉛的遺傳毒性及致癌性危害[J];國外醫(yī)學(xué)(衛(wèi)生學(xué)分冊);1996年03期

3 邵梅，姚華，張燕，，姜厚波，張小朋，萬伯健;鉛對女工自然流產(chǎn)及子女出生體重的影響[J];工業(yè)衛(wèi)生與職業(yè)病;1996年03期

4 賓萍;鄭玉新;;γ-H2AX與DNA雙鏈斷裂關(guān)系的研究進展[J];衛(wèi)生研究;2007年04期

5 田琳,黃士斌;應(yīng)用X熒光分析法測定體內(nèi)骨鉛含量方法初探[J];中國職業(yè)醫(yī)學(xué);2003年05期

6 梁建成;汪春紅;張妍;張春蓮;王程強;;醋酸鉛染毒小鼠DNA損傷及體內(nèi)抗氧化酶變化[J];中國公共衛(wèi)生;2006年04期

7 靳翠紅,呂相征,鄭麗舒,奚奇,胡連鑫;32例兒童血鉛和骨鉛水平的測定和淺析[J];中國醫(yī)科大學(xué)學(xué)報;2001年06期

8 田琳;體內(nèi)骨鉛含量測量的研究進展[J];中華勞動衛(wèi)生職業(yè)病雜志;2002年05期

9 周建華;薛蓮;時夕金;彭柳明;卞琛;;醋酸鉛致大鼠血淋巴細(xì)胞DNA損傷的研究[J];中華勞動衛(wèi)生職業(yè)病雜志;2006年05期

本文編號：1850808